[1]寇艳茹,苏淑钗. 授粉方式对板栗胚胎发育的影响[J].西北林学院学报,2015,30(03):94-99.[doi:doi:10.3969/j.issn.1001-7461.2015.03.17]
　KOU Yan-ru,SU Shu-chai. Effects of Pollination Methods on Chestnut Embryonic Development[J].JOURNAL OF NORTHWEST FORESTRY UNIVERSITY,2015,30(03):94-99.[doi:doi:10.3969/j.issn.1001-7461.2015.03.17]
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 授粉方式对板栗胚胎发育的影响()
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《西北林学院学报》[ISSN:1001-7461/CN:61-1202/S]

卷:
第30卷
期数:
2015年03期
页码:
94-99
栏目:
出版日期:
2015-05-31

文章信息/Info

Title:
 Effects of Pollination Methods on Chestnut Embryonic Development
文章编号:
1001-7461(2015)03-0094-06
作者:
 寇艳茹; 苏淑钗
 (北京林业大学，北京 100083)
Author(s):
 KOU Yan-ru;  SU Shu-chai
 (Beijing Forestry University, Beijing 100083, China)
关键词:
 ; 板栗; “遵玉”; 胚珠; 总苞; 子房; 氮素; 碳水化合物
Keywords:
 ; Chestnut;  Zunyu;  ovule;  involucre;  ovary;  nitrogen;  carbohydrate
分类号:
S718.43
DOI:
doi:10.3969/j.issn.1001-7461.2015.03.17
文献标志码:
A
摘要:
 为研究受精对板栗胚胎发育的影响，以“遵玉”品种10年生树为试验材料，通过控制授粉试验，观察不同授粉处理板栗胚珠、总苞和子房的形态发育及其氮素和碳水化合物含量的变化趋势。结果表明，受精不良导致空苞的产生，人工授粉板栗空苞率为0%，自然授粉板栗空苞率为12.37%，不授粉板栗空苞率为100%。在板栗果实发育早期，不授粉板栗与人工授粉、自然授粉板栗的胚珠大小、总苞和子房外观形态及碳水化合物含量变化差异不显著，但其氮素含量显著小于其他2个授粉处理板栗；授粉后16 d，正常发育胚珠大小显著大于发育异常胚珠，此时人工授粉、自然授粉板栗总苞和子房体积显著大于不授粉的板栗子房，碳水化合物含量差异不显著；幼胚发育期，人工授粉、自然授粉板栗子房可溶性糖、蔗糖和淀粉含量显著高于不授粉板栗子房的含量。
Abstract:
 In order to study the impact of fertilization on chestnut embryonic development, with chestnut cultivar “Zunyu” as test material, a controlled pollination experiment was conducted to observe impacts of different pollination treatments on chestnut ovule, involucre and ovary development, and variation trends of nitrogen and carbohydrate contents. The results showed that the empty shell rates the treatments of artificial-pollination, natural-pollination, and non-pollination were 0%, 12.37%, and 100%, respectively. Poor fertilization resulted in the generation of an empty shell. In early stage of pollination, ovule size, involucre and ovary morphology and carbohydrate content of non-pollination chestnut were not significant with artificial-pollination and natural-pollination chestnut, but its nitrogen content was significantly lower than the other two pollination treatments. Sixteen days after pollination, the normal development of the ovule size was significantly larger than the size of dysplasia ovule, at this time, artificial-pollination, natural-pollination chestnut involucre and ovary were significantly larger than those of the non-pollination, the carbohydrate content was not significantly different. In the period of immature embryo development, in the artificial-pollination and natural-pollination chestnut ovary, soluble sugars, sucrose and starch contents were significantly higher than that of the non-pollination chestnut. Therefore, to reduce the rate of empty shell, it is recommended to strengthen the chestnut flowering pollination work.

参考文献/References:

 [1]郝荣庭，刘孟军．中国干果[M]．北京：中国林业出版社，2005．
[2]张宇和，柳鎏，粱维坚，等．中国果树志·板栗榛子卷[M]．北京：中国林业出版社，2005．
[3]王祥坤，阮飞虎，张忠良，等．喷施硼肥对降低板栗空苞率及增产效果的影响[J]．西北林学院学报，2008，23（3）：130-132．
WANG X K，RUAN F H，ZHANG Z L，et al．Effects of application of boron on reducing the empty shell rate and increasing yield of Castanea mollissima nut[J]．Journal of Northwest Forestry University，2008，23(3) ：130-132．(in Chinese)
[4]杨柳，赵志珩，石卓功．板栗花粉直感效应对果实含糖量的影响[J]．西北林学院学报，2012，27(6)：75-77．
YANG L，ZHAO Z H，SHI Z G．Metaxenia effects on sugar content in Chinese chestnut (Castanea mollissima)[J]．Journal of Northwest Forestry University，2012，27(6)：75-77．(in Chinese)
[5]许慧玲，曹慧娟，李天庆．板栗(Castanea mollissima BL．)的胚胎学研究（Ⅰ）胚珠、胚囊发育、受精和胚发生[J]．北京林业大学学报，1988，10（1）：10-16．
XU H L，CAO H J， LI T Q． Study on the embryology of chestnut (Castanea mollissima BL．)Ⅰthe development of ovules and the embryo sac，fertilization and embryogenesis[J]．Journal of Beijing Forestry University， 1988，10（1）：10-16．(in Chinese)
[6]杜国华，周良骝，谢中稳，等．板栗空苞机理的研究[J]．果树学报，1995，12（1）：5-9．
[7]BOTTA R, VERGANO G, ME G, et al．Floral biology and embryo development in chestnut (Castanea sativa Mill．)[J]．Hortscience，1995，30(6) ：1283．
[8]王倩．品种配置对燕山板栗结实情况及果实品质影响研究[D]．北京：北京林业大学，2012．
[9]陆志科，谢碧霞．不同种竹叶的化学成分及其提取物抗菌活性的研究[J]．西北林学院学报，2005，20（1）：49-52．
LU Z K，XIE B X．A study of chemical composition and antimicrobial activity of different extracts in different kinds of Bamboo leaves[J]．Journal of Northwest Forestry University，2005，20（1）：49-52．(in Chinese)
[10]李合生．植物生理生化实验原理和技术[M]．北京：高等教育出版社，2000．
[11]夏仁学，马梦亭．板栗（Castanea millissima BL．）空苞形成因子的研究（Ⅰ）授粉受精对板栗空苞形成的影响[J]．华中农业大学学报，1989，8（3）：242-247．
XIA R X，MA M T．Study on factors of affecting empty-bur formation of Chinese chestnut (Castanea mollissima BL．) (Ⅰ) effects of pollination and fertilization on empty-bur formation of Chinese chestnut [J]．Journal of Huazhong Agricultural University，1989，8（3）：242-247．(in Chinese)
[12]杜桂森，黄勤妮，李增彬，等．北京地区板栗胚珠发育的研究[J]．北京师范学院学报:自然科学版，1992，13（3）：51-54．
DU G S，HUANG Q N，LI Z B，et al．On the ovule growth of chestnut in Beijing area[J]．Journal of Beijing Teachers College:Natural Science Edition，1992，13（3）：51-54．(in Chinese)
[13]姚家琳，夏仁学，马梦亭，等．板栗（Castanea millissima BL．）空苞形成因子的研究（Ⅱ）板栗空苞的胚胎学[J]．华中农业大学学报，1989，8（3）：248-253．
YAO J L，XIA R X，MA M T，et al．Study on factors of affecting empty-bur formation of Chinese chestnut (Castanea mollissima BL．)(Ⅱ) embryology of empty-bur[J]．Journal of Huazhong Agricultural University，1989，8（3）：248-253．(in Chinese)
[14]田寿乐，许林，沈广宁，等．板栗果实发育观察初报[J]．中国果树，2012（5）45-47．
[15]刘国彬，兰彦平，曹均．中国板栗生殖生物学研究进展[J]．果树学报，2011，28（6）：1063-1070．
[16]卓燕，郑强卿，窦中江，等．氮素营养代谢对果树生长发育的影响[J]．新疆农垦科技，2009(6)：41-43．
[17]魏丽红，翟秋喜．榛子果实发育中氮、磷元素动态变化[J]．安徽农业科学，2013，41（2）：650-651．
[18]彭福田，姜远茂，顾漫如，等．氮素对苹果果实内源激素变化动态与发育进程的影响[J]．植物营养与肥科学报，2003，9（2）：208-213．
PENG F T，JIANG Y M，GU M R，et al．Effect of nitrogen on apple fruit hormone changing trends and development[J]．Plant Nutrition and Fertilizer Science，2003，9（2）：208-213．(in Chinese)
[19]KOCH K．Sucrose metabolism：regulatory mechanisms and pivotal roles in sugar sensing and plant development[J]．Curr. Opin. Plant Biol.，2004，7(3)：235-246．
[20]谢鹏，郭素娟，熊欢，等．板栗果实糖和淀粉积累及相关酶活性关系的研究[J]．园艺学报，2012，39（12）：2369-2376．
XIE P，GUO S J，XIONG H，et al．Changes in sugar, starch, some enzymes involved and their relationships during the development of Chinese chestnut[J]．Acta Horticulturae Sinaca，2012，39（12）：2369-2376．(in Chinese)
[21]周志翔，夏仁学，章文才，等．板栗果实生长与子房主要营养物质积累的关系研究[J]．华中农业大学学报，2000，19（1）：74-78．
ZHOU Z X，XIA R X，ZHANG W C，et al．Study on the relationship between fruit growth and main nutritious substances accumulation in ovary of Chinese chestnut [J]．Journal of Huazhong Agricultural University，2000，19（1）：74-78．(in Chinese)
[22]梁文裕，许鸿川，陈伟，等．龙眼胚胎发育过程中胚珠内核酸、蛋白质和淀粉含量的变化[J]．福建农林大学学报:自然科学版，2006，35（1）：38-41．
LIANG W Y，XU H C，CHEN W，et al．Changes of nucleic acid，protein and starch content in ovule during the longan’s embryo development[J]．Journal of Fujian Agriculture and Forestry University:Natural Science Edition，2006，35（1）：38-41．(in Chinese)
[23]周良骝，杜国华，谢中稳，等．板栗发育过程中主要营养物质变化规律的研究[J]．安徽农业大学学报，1994，21（4）：441-445.

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　WAN G Xi angkun,Y U AN Feihu,Z HAN G Z hongli ang,et al.Effects of Application of Boronon Reduci ng t he Empt y Shell Rat e and Increasi ng Yield of Castane amollisssimanut[J].JOURNAL OF NORTHWEST FORESTRY UNIVERSITY,2008,23(03):130.
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备注/Memo

备注/Memo:
 收稿日期：2014-12-08 修回日期：2015-01-20
基金项目：国家“十一五”科技支撑计划项目(2006BAD01A1703-1)。
作者简介：寇艳茹，女，在读硕士，研究方向：经济林栽培。E-mail:610922451@qq.com
*通信作者：苏淑钗，女，教授，博士，研究方向：经济林良种选育及高效栽培技术。E-mail:sushuchai@sohu.com
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