[1]徐毅励,陈媛梅*. 重瓣榆叶梅花中蛋白质提取工艺及动态含量研究[J].西北林学院学报,2015,30(03):191-196.[doi:doi:10.3969/j.issn.1001-7461.2015.03.33]
　XU Yi-li,CHEN Yuan-mei*. Optimal Extraction Technology and Dynamic Content of Protein in the Flower of Amygdalus triloba f. multiplex[J].JOURNAL OF NORTHWEST FORESTRY UNIVERSITY,2015,30(03):191-196.[doi:doi:10.3969/j.issn.1001-7461.2015.03.33]
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 重瓣榆叶梅花中蛋白质提取工艺及动态含量研究()
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《西北林学院学报》[ISSN:1001-7461/CN:61-1202/S]

卷:
第30卷
期数:
2015年03期
页码:
191-196
栏目:
出版日期:
2015-05-31

文章信息/Info

Title:
 Optimal Extraction Technology and Dynamic Content of Protein in the Flower of Amygdalus triloba f. multiplex
文章编号:
1001-7461(2015)03-0191-06
作者:
 徐毅励; 陈媛梅*
 （北京林业大学 理学院，北京100083）
Author(s):
 XU Yi-li;  CHEN Yuan-mei*
 (College of Science, Beijing Forestry University, Beijing 100083, China)
关键词:
 ; 重瓣榆叶梅花; 超声提取; 蛋白质; 液料比; 动态含量
Keywords:
Amygdalus triloba f. multiplex;  ultrasonic wave aided extraction;  protein;  liquid ratio;  dynamic content
分类号:
S713
DOI:
doi:10.3969/j.issn.1001-7461.2015.03.33
文献标志码:
A
摘要:
 重瓣榆叶梅花中蛋白质提取研究表明，最佳提取工艺为采用pH7.0的磷酸氢二钠提取液、液料比为30∶1（mL∶g）、30℃、500 W、超声提取3次(每次120 min)。在该提取工艺条件下，测得开花14 d花中蛋白质含量最高,为13.237 mg·g-1；开花21 d花中蛋白质含量最低，仅为5.150 mg·g-1。
Abstract:
 Crude proteins were extracted from the flowers of Amygdalus triloba f. multiplex. The optimal conditions of the extraction were obtained: Solvent: disodium hydrogen phosphate with pH=7.0, ration of solvent to material:30∶1（mL∶g）. The extraction was conducted for 3 times (120 min each) at 30℃ aided by ultrasonic wave (500 W of ultrasonic generator power). By above extraction conditions, it was found the highest content of protein was in the flowers collected at the 14th day after blossoming, about 13.237 mg·g-1, lowest at the 21st day, about 5.150 mg·g-1.

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备注/Memo

备注/Memo:
 收稿日期：2014-09-05 修回日期：2014-11-04
基金项目：国家自然科学基金（30671655）。
作者简介：徐毅励，男，在读硕士，研究方向：生物物理专业，大分子结构。E-mail:xuyiliqq@126.com
*通信作者：陈媛梅，女，副教授，研究方向：天然化合物的提取分离与物理化学性质。E-mail：chym11@bjfu.edu.cn
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