[1]郭鑫,梁宗锁*,解娟芳,等. 秦艽主要药性化学物质指纹图谱分析[J].西北林学院学报,2015,30(03):197-200.[doi:doi:10.3969/j.issn.1001-7461.2015.03.34]
　GUO Xin,LIANG Zong-suo*,XIE Juan-fang,et al. HPLC Fingerprint Analysis of Gentiana macrophylla[J].JOURNAL OF NORTHWEST FORESTRY UNIVERSITY,2015,30(03):197-200.[doi:doi:10.3969/j.issn.1001-7461.2015.03.34]
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 秦艽主要药性化学物质指纹图谱分析()
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《西北林学院学报》[ISSN:1001-7461/CN:61-1202/S]

卷:
第30卷
期数:
2015年03期
页码:
197-200
栏目:
出版日期:
2015-05-31

文章信息/Info

Title:
 HPLC Fingerprint Analysis of Gentiana macrophylla
文章编号:
1001-7461(2015)03-0197-04
作者:
 郭鑫1; 梁宗锁1; 2*; 解娟芳3; 马志科3
 （1.西北农林科技大学 生命科学学院，陕西 杨陵 712100；2.浙江理工大学 生命科学学院，浙江 杭州 310018；3.宁夏隆德西北药材科技有限公司，宁夏 隆德 756300）
Author(s):
 GUO Xin1;  LIANG Zong-suo1; 2*;  XIE Juan-fang3;  MA Zhi-ke3
 (1. College of Life Sciences, Northwest Ａ＆Ｆ University, Yangling , Shaanxi 712100, China; 2. College of Life Sciences，Zhejiang Sci-Tech University，Hangzhou, Zhejiang 310018, China; 3.The Northwest Medicine Science and Technology Limited, Longde, Ningxia 756300, China)
关键词:
 ; 秦艽; HPLC; 指纹图谱; 龙胆苦苷; 马钱苷酸
Keywords:
Gentiana macrophylla;  HPLC;  fingerprint;  gentiopicrin;  loganin acid
分类号:
S713
DOI:
doi:10.3969/j.issn.1001-7461.2015.03.34
文献标志码:
A
摘要:
 以8个不同地区秦艽的干燥根为试验材料，以Alltech Chrom BDS-C18色谱柱（4.6 mm×150 mm，5 μm）为色谱柱，乙腈—0.1%醋酸溶液（9∶91）为流动相进行等度洗脱，检测波长254 nm；柱温30℃，进样量10 μL。通过中药色谱指纹图谱相似度评价系统（国家药典委员会）进行相似度分析，建立8个不同产地的秦艽指纹图谱。以宁夏六盘山区所产秦艽为例，通过高效液相色谱法，以龙胆苦苷、马钱苷酸2个主要成分为主要因素，分析适宜秦艽有效成分积累的最佳立地条件，为建立秦艽质量标准、选择最佳生长条件提供理论依据。结果表明：1)8个不同产地秦艽指纹图谱共有6个主要共有峰，可作为鉴别秦艽药材的主要依据。2) 8个不同地区秦艽药材聚类分析可分为4类：内蒙古海拉尔和山西阳泉的秦艽药材为一类,青海同仁和内蒙古通辽的为一类,河北张家口和陕西太白的为一类,云南洱源和宁夏六盘山的为一类。3)通过对宁夏六盘山区不同的秦艽生长立地条件的探究，有效成分积累量最高为阳坡，最低为半阴坡。
Abstract:
 In order to establish the chromatographic fingerprints, the dried Gentiana macrophylla roots collected from different regions were used as materials to carry out high performance liquid chromatography (HPLC). The related chromatogranphic conditions were as follows: column: Alltech Chrom BDS-C18（4.6 mm×150 mm，5 μm, mobile phase: acetonitrile∶0.1% acetic acid (9∶91) for isocratic elution; flow rate: 1.0 mL·min-1; detection wavelength: 254 nm: column temperature: 30℃. Chromatographic fingerprints of G. macrophylla from 8 regions were established based on the analysis of their similarities. In order to establish the quality standard and provide theoretical basis for the selection of optimal growth conditions, taking the sample collected from Liupan Mountain of Ningxia as a specific case, and using two effective constituents, gentiopicrin and loganin acid as the main factors, the optimal site conditions for the accumulation of the constituents were discussed. The result indicates that 1) a total of 6 main common peaks was found in the fingerprints of roots of G. macrophylla from 8 different regions, which could be used as the main basis for the identification of G. straminea Maxim. 2) According to the clustering analysis, the roots could be divided into 4 categories: those from Hailaer of Inner Mongonia and Yangquan of Shanxi, Tongren of Qinghai and Tongliao of Inner Mongolia, Zhangjiakou of Hebei and Taibai of Shaanxi, Eryuan of Yunnan and Liupan Mountain of Ningxia. 3) It was found that the sunny slope was favorable for the accumulation of effective constituents, while the half shady slope was the worst.

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备注/Memo

备注/Memo:
 收稿日期：2015-02-16 修回日期：2015-03-24
基金项目：宁夏自然科学基金（NZ13207）。
作者简介：郭鑫，男，在读硕士，研究方向：药用植物资源与利用。E-mail：guoxin_master_2013@yeah.net
*通信作者：梁宗锁，教授，博士生导师，研究方向：药用植物资源与利用。E-mail：liangzs@ms.iswc.ac.cn
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