[1]李珍,吕平会,杜双田*,等. 植物生长调节剂对灰树花QY-01菌丝生长的影响[J].西北林学院学报,2016,31(2):188-194.[doi:10.3969/j.issn.1001-7461.2016.02.32]
 LI Zhen,LYU Ping-hui,DU Shuang-tian*,et al. Effect of Different Types of Plant Growth Regulators on the Hypha Growth of Grifola frondosa[J].JOURNAL OF NORTHWEST FORESTRY UNIVERSITY,2016,31(2):188-194.[doi:10.3969/j.issn.1001-7461.2016.02.32]
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 植物生长调节剂对灰树花QY-01菌丝生长的影响()
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《西北林学院学报》[ISSN:1001-7461/CN:61-1202/S]

卷:
第31卷
期数:
2016年第2期
页码:
188-194
栏目:
出版日期:
2016-03-31

文章信息/Info

Title:
 Effect of Different Types of Plant Growth Regulators on the Hypha Growth of Grifola frondosa
文章编号:
1001-7461(2016)02-0188-07
作者:
 李珍1吕平会2杜双田1*丁建1鲁车龙1
 (1.西北农林科技大学 生命科学学院,陕西 杨陵 712100;2.西北农林科技大学 林学院,陕西 杨陵 712100)
Author(s):
 LI Zhen1LYU Ping-hui2DU Shuang-tian1*DING Jian1LU Che-long1
 (1.College of Life Science,Northwest A&F University,Yangling,Shaanxi 712100,China;2.College of Forestry,Northwest A&F University,Yangling,Shaanxi 712100,China)
关键词:
 灰树花植物生长调节剂菌落直径
Keywords:
Grifola frondosa plant growth regulator colony diameter
分类号:
S763.15
DOI:
10.3969/j.issn.1001-7461.2016.02.32
文献标志码:
A
摘要:
 以灰树花菌株QY-01为材料,在添加不同种类及浓度植物生长调节剂的培养基上培养,以灰树花的菌落直径为主要指标。研究植物生长调节剂对灰树花菌丝生长的影响规律,筛选促进灰树花菌丝生长的植物生长调节剂及其适宜使用浓度。结果表明,KT-30和6-BA对白灵菇菌丝生长的促进作用强,芸苔素和赤霉素对其菌丝生长无作用,NAA、三十烷醇、DA-6等的促进作用较弱;菌丝对6-BA非常敏感,对NAA表现较强耐受性,高浓度的KT-30和6-BA导致菌落形态改变;KT-30、6-BA、NAA、三十烷醇、鲜胺酯、IAA、低聚壳聚糖、四甲基戊二酸和复硝酚钠对菌落直径的提高比例分别为57.9%、52.4%、11.7%、10.9%、10.2%、10.0%、8.5%、8.4%和8.1%,最佳浓度分别为4.9、10.0、5.0、0.1、3.7、3.6、7.5、6.5 mg/L和2.7 mg/L,对灰树花的液体发酵及液体菌种培养具有重要意义。
Abstract:
Grifola frondosa strain QY-01was cultivated on the media supplemented with different types and concentrations of plant growth regulators to examine their effects on strain growth and to select optimal regulator and concentration by using colony diameter as index.The results showed that KT-30 and 6-BA had obvious promoting effect on the mycelium growth,while NAA,triacontanol,and DA-6 were weaker.Canola and GA3 had no effect.The stain was very sensitive to 6-BA,and showed strong tolerance to NAA.High concentrations of KT-30 and triacontanol caused changes in the colony morphology.Compared with controls,the colony diameters of KT-30,6-BA,NAA,Triacontanol,DA-6,IAA,Chitosan,Tetramethyl gluttaric aciduria and Sodium nitrophenolate treated sample significantly increased by 57.9%,52.4%,11.7%,10.9%,10.2%,10%,8.5%,8.4% respectively,the corresponding optimal concentrations were 4.9,10.0,5.0,0.1,3.7,3.6,7.5 ,6.5 mg/L and 2.7 mg/L,respectively.The result was of great significance to liquid fermentation and the strains cultivation of G.frondosa.

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备注/Memo

备注/Memo:
 收稿日期:2015-05-06修回日期:2015-06-01
基金项目:“十二五”科技支撑项目“西北地区板栗高效生产关键技术研究与示范”(2013BAD14B0404);国家林业局重点推广课题“板栗新品种栽培技术集成与示范推广”[2014] 44号。
作者简介:李珍,女,在读硕士,研究方向:微生物资源开发与利用。E-mail:lizhennew2008@163.com
*通信作者:杜双田,男,副教授,研究方向:食用与药用真菌。E-mail:dst6107@126.com
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