[1]刘冬明,袁德义*,邹锋,等. 3种锥栗外生菌根真菌培养条件的单因素优化[J].西北林学院学报,2016,31(2):195-200.[doi:10.3969/j.issn.1001-7461.2016.02.33]
　LIU Dong-ming,YUAN De-yi*,ZOU Feng,et al. Optimization of Culture Conditions for 3 Castanea henryi Ectomycorrhizal fungi[J].JOURNAL OF NORTHWEST FORESTRY UNIVERSITY,2016,31(2):195-200.[doi:10.3969/j.issn.1001-7461.2016.02.33]
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 3种锥栗外生菌根真菌培养条件的单因素优化()
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《西北林学院学报》[ISSN:1001-7461/CN:61-1202/S]

卷:
第31卷
期数:
2016年第2期
页码:
195-200
栏目:
出版日期:
2016-03-31

文章信息/Info

Title:
 Optimization of Culture Conditions for 3 Castanea henryi Ectomycorrhizal fungi
文章编号:
1001-7461(2016)02-0195-06
作者:
 刘冬明1; 2; 袁德义1; 2*; 邹锋1; 2; 张旭辉1; 2; 朱周俊1; 2; 谭露曼1; 2
 (1.中南林业科技大学 经济林培育与保护教育部重点实验室，湖南 长沙 410004；2.中南林业科技大学 经济林育种与栽培国家林业局重点实验室，湖南 长沙 410004)
Author(s):
 LIU Dong-ming1; 2; YUAN De-yi1; 2*; ZOU Feng1; 2; ZHANG Xu-hui1; 2; ZHU Zhou-jun1; 2; TAN Lu-man1; 2
 (1.The Key Lab of Non-wood Forest Nurturing and Protection of the Nation Ministry of Edcuation,Central South University of Forestry and Technology,Changsha,Hunan 410004,China;2.The Key Laboratory of Non-timber Breeding and Cultivation of State Forestry Administration,Changsha,Hunan 410004,China)
关键词:
 ; 锥栗; 外生菌根真菌; 培养条件
Keywords:
Castanea henryi;  ectomycorrhizal fungus;  culture condition
分类号:
S763.15
DOI:
10.3969/j.issn.1001-7461.2016.02.33
文献标志码:
A
摘要:
 明确3种锥栗外生菌根真菌的最适培养条件，为锥栗外生菌根真菌进一步研究和生产应用奠定基础。采用固体培养方法，用十字交叉法测量菌落的直径，研究不同温度（15、20、25、30℃和35℃）、pH值（4.0、5.0、6.0、7.0、8.0）、碳源（葡萄糖、蔗糖、甘露醇、麦芽糖、可溶性淀粉）、氮源（KNO3、（NH4）2HPO4、NH4NO3、蛋白胨、酵母膏）对3种锥栗外生菌根真菌生长的影响。结果表明，3种锥栗外生菌根真菌最佳生长条件差异不大，最适生长温度均为25℃，属中温菌；3种外生菌根真菌在pH4.0~8.0均能生长，鸡枞菌(Termitornyces albuminosus)和正红菇(Russula griseocarnosa)最适生长pH为6.0，橙黄硬皮马勃(Scleroderma citrinum)为7.0；3种外生菌根真菌的最适碳源均为甘露醇，最适氮源均为KNO3。
Abstract:
 The objective of this research was to find out the optimum culture conditions of 3 ectomycorrhizal fungi for the practical application and bacteria production.Ectomycorrhizal fungi were cultured in solid state,and the colony diameter was measured by cross the crossover method.Effects of temperatures (15,20,25,30℃ and 35℃),pH (4.0,5.0,6.0,7.0 and 8.0),carbon sources (glucose,sucrose,mannitol,malt sugar and soluble starch),nitrogen sources (KNO3,(NH4)2HPO4,NH4NO3,peptone and yeast extract) on the fungus growth were studied.The results indicated that the optimum culture conditions of 3 ectomycorrhizal fungi were slightly different.All of them grew fast at 25℃,and they belonged to moderate temperature fungi.The 3 ectomycorrhizal fungi could grow in pH ranging from 4.0~8.0,while the optimun pH value of Termitornyces albuminosus and Russula griseocarnosa was 6.0,Scleroderma citrinum was 7.0.The optimum carbon source of all the 3 ectomycorrhizal fungi was mannitold and the optimum nitrogen source was KNO3.

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备注/Memo

备注/Memo:
 收稿日期：2015-06-18修回日期：2015-07-19
基金项目：国家“十二五”科技支撑计划课题“板栗和锥栗高效生产关键技术研究与示范”(2013BAD14B04)。
作者简介：刘冬明，女，硕士研究生，研究方向：果树育种与栽培。E-mail:ldm19890321@163.com。
*通信作者：袁德义，男，教授，博士生导师，研究方向：经济林育种与栽培。E-mail:yuan-deyi@163.com
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