[1]李畅,傅建敏*,王森,等. 阳丰甜柿组织培养再生体系的建立[J].西北林学院学报,2016,31(3):159-164.[doi:10.3969/j.issn.1001-7461.2016.03.27]
　LI Chang,FU Jian-min*,WANG Sen,et al. Establishment of Tissue Culture Regeneration System of Diospyros kaki cv.Youhou[J].JOURNAL OF NORTHWEST FORESTRY UNIVERSITY,2016,31(3):159-164.[doi:10.3969/j.issn.1001-7461.2016.03.27]
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 阳丰甜柿组织培养再生体系的建立()
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《西北林学院学报》[ISSN:1001-7461/CN:61-1202/S]

卷:
第31卷
期数:
2016年第3期
页码:
159-164
栏目:
出版日期:
2016-05-31

文章信息/Info

Title:
 Establishment of Tissue Culture Regeneration System of Diospyros kaki cv.Youhou
文章编号:
1001-7461(2016)03-0159-05
作者:
 李畅1; 傅建敏2*; 王森1; 文亚峰1; 晏巢3; 孙鹏2; 刁松峰2; 韩卫娟2
 （1.中南林业科技大学 林学院，湖南 长沙 410004；2.国家林业局 泡桐研究开发中心，河南 郑州 450003；3.中国林科院 亚热带林业实验中心，江西 分宜 336600）
Author(s):
 LI Chang1; FU Jian-min2*; WANG Sen1; WEN Ya-feng1; YAN Chao3; SUN Peng2; DIAO Song-feng2; HAN Wei-juan2
 (1.College of Forestry，Central South University of Forestry and Technology,Changsha,Hunan 410004,China; 2.Paulownia Research＆Development Center,State Forestry Administration,Zhengzhou,Henan 450003,China; 3.The Experimental Centre of Subtropical Forestry,Fenyi,Jiangxi 336600,China)
关键词:
 ; 阳丰甜柿; 组织培养; 腋芽诱导; 再生体系
Keywords:
Diospyros kaki cv.Youhou;  tissue culture;  axillary bud induction;  regeneration system
分类号:
S723.1
DOI:
10.3969/j.issn.1001-7461.2016.03.27
文献标志码:
A
摘要:
 以阳丰甜柿(Diospyros kaki cv.Youhou)的带腋芽茎段为外植体，研究了植物生长调节剂及其浓度配比对其腋芽的诱导、增殖及生根的影响。结果表明，带芽茎段接种在1/2MS+1.0 mg·L-1ZT+0.1 mg·L-1IAA培养基培养4周后，腋芽诱导率为77.8%。将萌发的腋芽切成带芽茎段或单株转到1/2MS+1.0 mg·L-1ZT+2.0 mg·L-12ip+0.1 mg·L-1IAA培养基继代培养，最高增殖系数可达4.1。在增殖培养中长至3 cm以上的芽转入1/2MS+0.75 mg·L-1IBA的生根培养基中暗培养7 d后，转入光下培养30 d，生根率可达73.3%，平均生根数为3.6。该研究建立了阳丰甜柿离体再生体系，为其规模化生产提供了技术平台。
Abstract:
 Taking the stem segments with auxiliary buds of Diospyros kaki cv.Youhou as explants,influences of plant regulators and their concentrations on the induction,proliferation and rooting were examined.The induction rate of auxiliary bud was 77.8% after the explants were cultured 4 weeks in the medium of 1/2MS+1.0Mg·L-1Zeatin+0.1 mg·L-1IAA.The regenerated buds were then subcultured in the medium of 1/2MS+1.0 mg·L-1ZT+2.0 mg·L-12ip+0.1 mg·L-1IAA,the highest proliferation coefficient was 4.1.The shoots with 3 cm in length were transferred into the medium 1/2 MS+0.75 mg·L-1IBA,and cultured for 7 d in dark and 30 d in light,the rooting rate was 73.3%,and the average number of root was 3.6.This results established the in vitro propagation system for Diospyros kaki,and provided the technical basis for scale production.

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备注/Memo

备注/Memo:
 收稿日期：2015-11-08修回日期：2015-12-28
基金项目：国家“十二五”农村领域科技计划课题（2013BAD14D0502）。
作者简介：李畅，女，在读硕士，研究方向：经济林栽培与育种。E-mail:846770189@qq.com
*通信作者：傅建敏，女，副研究员，研究方向：经济林栽培与育种。E-mail:fjm371@163.com
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