[1]罗洁,杨国*,陈红锋. 双片苣苔的离体培养[J].西北林学院学报,2016,31(3):165-169.[doi:10.3969/j.issn.1001-7461.2016.03.28]
　LUO Jie,YANG Guo*,CHEN Hong-feng. Rapid Propagation in vitro of Didymostigma obtusum[J].JOURNAL OF NORTHWEST FORESTRY UNIVERSITY,2016,31(3):165-169.[doi:10.3969/j.issn.1001-7461.2016.03.28]
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 双片苣苔的离体培养()
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《西北林学院学报》[ISSN:1001-7461/CN:61-1202/S]

卷:
第31卷
期数:
2016年第3期
页码:
165-169
栏目:
出版日期:
2016-05-31

文章信息/Info

Title:
 Rapid Propagation in vitro of Didymostigma obtusum
文章编号:
1001-7461(2016)03-0165-05
作者:
 罗洁1; 杨国2*; 陈红锋3
 （1.绍兴文理学院 元培学院，浙江 绍兴 312000；2.绍兴文理学院 生命科学院，浙江 绍兴 312000；3.中国科学院 华南植物园，广东 广州 510650）
Author(s):
 LUO Jie1; YANG Guo2*; CHEN Hong-feng3
 (1.Shaoxing University,Academy of Yuanpei,Shaoxing,Zhejiang 312000 China; 2.Shaoxing University,Academy of Life Science,Shaoxing,Zhejiang 312000,China; 3.South China Botanical Garden,Chinese Academy of Sciences,Guangzhou,Guangdong 510650,China)
关键词:
 ; 双片苣苔; 珍稀植物; 离体快繁; 组织培养
Keywords:
Didymostigma obtusum;  rare plant;  in vitro culture;  rapid propagation
分类号:
S722.89
DOI:
10.3969/j.issn.1001-7461.2016.03.28
文献标志码:
A
摘要:
 以珍稀植物双片苣苔（Didymostigma obtusum）叶片和叶柄为外植体，以MS为基本培养基，研究了外植体的消毒方式，不同生长调节剂及其组合对不定芽诱导及生根的影响，探讨不同光照、pH、叶片放置方式等因素对不定芽诱导的影响。结果表明：适量的链霉素有利于外植体消毒，叶片诱导不定芽发生的适宜培养基为MS+2.0 μmol·L-1 TDZ+0.2 μmol·L-1 NAA，叶柄诱导不定芽发生的适宜培养基为MS+2.0 μmol·L-1 TDZ+1.0 μmol·L-1 BA，pH为5.6~6.0，叶背面朝下接种有利于叶片不定芽的诱导发生，适当增强光照有利于芽苗的生长；适宜生根培养基为1/2MS+2 μmol·L-1 IBA+2 μmol·L-1 NAA，生根率达100%，根系生长较好；试管苗移栽到腐殖质和黄泥（2∶1）的混合基质中，置于半荫温室中，成活率达100%。
Abstract:
 Leaves and petioles of Didymostigma obtusum were used as explants for rapid propagation.The effects of different cultural conditions on the formation of adventitious buds were examined,such as illumination,pH,disinfection method,plant growth regulators and their combinations,explant orientation for induction.The results showed that streptomycin was beneficial to sterilization,the MS medium combined with 2.0 μmol·L-1 TDZ and 0.2 μmol·L-1 NAA was most suitable for shoot organogenesis from leaf explants,and the MS medium combined with 2.0 μmol·L-1 TDZ and 1.0 μmol·L-1 BA was the best for shoot organogenesis from petiole explants,leaf explants placed with adaxial side in contact induced more adventitious shoots.MS medium contained 2.0 μmol·L-1 TDZ and 0.2 μmol·L-1 NAA was the best for multiplication and growth of adventitious buds,the optimal pH was 5.6-6.0,growth of adventitious buds was improved by increasing light intensity.Rooting of shoots was achieved on medium on half-strength MS salts and supplemented with 2 μmol·L-1 IBA and 2 μmol·L-1 NAA.Plantlets were transplanted to potting mixture (1∶2,yellow soil∶humus) in half-shadow greenhouse with about 100% survival percentage.

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备注/Memo

备注/Memo:
 收稿日期：2015-12-02修回日期：2016-01-16
基金项目：广东省林业科技创新项目（2015KJCX037）；绍兴文理学院校级项目(2014LG1018)；绍兴文理学院元培学院院级项目(Z20150014)。 
作者简介：罗洁，女，硕士，研究方向：植物应用和植物造景。E-mail：lj26wlxy@163.com
*通信作者：杨国，男，硕士，研究方向：植物资源开发。E-mail：ygelite@163.com
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