[1]虎娟,张虹,蒋清安,等. 黑果枸杞花青素生物合成关键基因的生物信息学分析与克隆[J].西北林学院学报,2017,32(6):1-11.[doi:10.3969/j.issn.1001-7461.2017.06.01]
　HU Juan,ZHANG Hong,JIANG Qing-an,et al. Bioinformatics Analysis and Cloning of the Key Genes Associated with Anthocyanin Biosynthesis from Lycium ruthenicum[J].JOURNAL OF NORTHWEST FORESTRY UNIVERSITY,2017,32(6):1-11.[doi:10.3969/j.issn.1001-7461.2017.06.01]
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《西北林学院学报》[ISSN:1001-7461/CN:61-1202/S]

卷:
第32卷
期数:
2017年第6期
页码:
1-11
栏目:
出版日期:
2017-11-30

文章信息/Info

Title:
 Bioinformatics Analysis and Cloning of the Key Genes Associated with Anthocyanin Biosynthesis from Lycium ruthenicum
文章编号:
1001-7461(2017)06-0001-11
作者:
 虎娟; 张虹; 蒋清安; 林哲; 马伟宝; 陈任*
 （宁夏大学 西部特色生物资源保护与利用教育部重点实验室；宁夏大学 生命科学学院，宁夏 银川 750021）
Author(s):
 HU Juan; ZHANG Hong; JIANG Qing-an; LIN Zhe; MA Wei-bao; CHEN Ren*
 (Key Lab of the Ministry of Education for Protection and Utilization of Special Biological Resources in Western China,Ningxia University;School of Life Science,Ningxia University,Yinchuan,Ningxia 750021,China)
Keywords:
 ; anthocyanin biosynthesis;  transcriptome sequencing;  bioinformatics analysis;  PCR cloning;  Lycium ruthenicum
分类号:
S718.46
DOI:
10.3969/j.issn.1001-7461.2017.06.01
文献标志码:
A
摘要:
 花青素生物合成；转录组测序；生物信息学分析；PCR克隆；黑果枸杞
Abstract:
Lycium ruthenicum is a unique medicinal material in the northwestern China.It contains the highest content of anthocyanin.To understand more about the biosynthesis mechanism of anthocyanin,in the present research,seven key genes associated with anthocyanin biosynthesis in L.ruthenicum were identified using transcriptome sequencing.The genes including chalconesynthase,chalconeisomerase,clavonone-3-hydroxylase,flavonoid-3′-hydroxylase,flavonone-3′5′-hydroxylase,dihydvroflavonol-4-reductase and anthocyanidin synthase,and their bioinformatics in nucleotide sequences,amino acid sequences and their protein (enzyme) structures were characterized.Then the full lengths of the seven key genes were amplified by PCR according to the transcript assembly sequences,and were verified by sequencing.The research would provide a basis for genetic improvement of L.ruthenicum and the utilization of the genes involved in anthocyanin biosynthesis.

备注/Memo

备注/Memo:
 收稿日期：2017-06-22修回日期：2017-07-17
基金项目：2013年度宁夏回族自治区科技支撑计划项目（413-0396）。
作者简介：虎娟,女,在读硕士,研究方向:植物分子生理与生物技术。E-mail:380128339@qq.com
*通信作者：陈任,男,教授,博士,研究方向:植物分子生物学与基因工程。E-mail:chenren511@163.com
更新日期/Last Update: