[1]陈昕,陈蕊红,黄建*. 枣果实不同发育阶段蛋白质组动态研究[J].西北林学院学报,2019,34(4):82-90.[doi:10.3969/j.issn.1001-7461.2019.04.12]
　CHEN Xin,CHEN Rui-hong,HUANG Jian*. Comparative Proteomic Analysis on Chinese Jujube Fruit at Different Developmental and Ripening Stages[J].JOURNAL OF NORTHWEST FORESTRY UNIVERSITY,2019,34(4):82-90.[doi:10.3969/j.issn.1001-7461.2019.04.12]
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 枣果实不同发育阶段蛋白质组动态研究()
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《西北林学院学报》[ISSN:1001-7461/CN:61-1202/S]

卷:
第34卷
期数:
2019年第4期
页码:
82-90
栏目:
出版日期:
2019-07-31

文章信息/Info

Title:
 Comparative Proteomic Analysis on Chinese Jujube Fruit at Different Developmental and Ripening Stages
文章编号:
1001-7461(2019)04-0082-09
作者:
 陈昕1; 陈蕊红2; 黄建1*
 （1.西北农林科技大学 林学院，陕西 杨陵 712100；2.延安大学 生命科学学院 陕西省黄土高原特色植物与生态重点实验室，陕西 延安 716000）
Author(s):
 CHEN Xin1; CHEN Rui-hong2; HUANG Jian1*
 (1.College of Forestry,Northwest A&F University,Yangling 712100,Shaanxi,China; 2.College of Life Science,Shaanxi Key Lab.of Characteristic Plants and Ecology of Loess Plateau,Yanan University,Yanan 716000,Shaanxi,China)
关键词:
 ; 枣; 果实成熟; 非标记定量蛋白质组; 果实膨大; 果实糖积累
Keywords:
Ziziphus jujuba;  fruit ripening;  comparative proteomics;  fruit enlargement;  sugar accumulation
分类号:
S718.43
DOI:
10.3969/j.issn.1001-7461.2019.04.12
文献标志码:
A
摘要:
 枣果实在发育和成熟过程中涉及多种复杂的生理生化变化。为了解枣果实在发育过程中蛋白质组的变化情况，利用液质联用（LC-MS）技术对主栽制干枣品种——骏枣幼果期、膨大期、白熟期、初红期和全红期5个阶段的果实进行了非标记定量蛋白质组动态变化研究。结果表明，各时期果实与其前一阶段相比分别有差异表达蛋白189、356、82、69个。基因本体（GO）注释结果显示：幼果与膨大果的差异蛋白主要富集在核酸代谢过程，膨大期果实与白熟果的差异蛋白主要富集在碳水化合物代谢过程。从5个阶段的果实中分别鉴定到604、186、91、83、189个特异表达蛋白。Mercator注释和KEGG通路注释结果显示，幼果期特异表达蛋白主要参与了RNA转运通路。加权基因共表达网络（WGCNA）分析显示，幼果期高表达蛋白主要与核糖体合成相关。总之，枣果实在白熟期以前与细胞分裂和膨大相关的蛋白表达量和富集程度高，从白熟期开始与碳水化合物积累相关的蛋白质表达上调。本研究从蛋白质组学水平阐明了枣果实发育成熟过程中蛋白质组分的动态变化，为提高枣果实品质奠定了理论基础。
Abstract:
 In order to understand the dynamic process of the proteomics during the progress of jujube fruit development and ripening,we quantitatively analyzed the proteomics of jujube fruits at 5 developmental stages (young,enlarging,white mature,early red,and fully red) using high performance liquid chromatography-mass spectrometry method.The results showed that compared with the former stage,there existed 189,356,82,69 differentially expressed proteins,respectively in the fruit of the last four stages.The gene ontology annotation showed that the differential proteins between young and enlarging fruit mainly enriched in nucleic acid metabolism,and the differential proteins between enlarging fruit and white mature fruit were mainly involved in carbohydrate metabolism.We also identified 604,186,91,83,189 expressed proteins from the fruit of five stages,respectively.Mercator annotation and KEGG pathway annotation revealed that those specifically expressed proteins in young fruit were mainly involved in the RNA transport pathway.WGCNA analysis showed that the highly expressed proteins in the young fruit were mainly related to ribosome biogenesis.In general,prior to the white mature stage,the jujube fruit mainly underwent cell division and enlargement,while the fruit mainly underwent carbohydrate accumulation after the white ripening stage.The dynamic of proteins during jujube fruit development and maturity were elucidated from proteomics level,and therefore laid a theoretical foundation for improving the quality of jujube fruit.

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备注/Memo

备注/Memo:
 收稿日期：2019-03-07修回日期：2019-04-07
基金项目：新疆生产建设兵团科技重点项目（2017DB006-1）；国家自然科学基金（31870584）。
作者简介：陈昕，男，硕士在读，研究方向：森林培育。E-mail:chenxin0526@126.com
*通信作者：黄建，男，博士，副研究员，研究方向：经济林培育与育种。E-mail:huangj@nwsuaf.edu.cn
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