[1]袁斌玲,王瑞敏,陈颖*,等. NaCl处理下茉莉酸甲酯对金叶银杏光合色素、抗氧化性及黄酮代谢的调控作用[J].西北林学院学报,2020,35(2):64-71.[doi:10.3969/j.issn.1001-7461.2020.02.09]
　YUAN Bin-ling,WANG Rui-min,CHEN Ying*,et al. The Regulation of Methyl Jasmonate on Photosynthetic Pigments,Antioxidation and Flavonoid Metabolism in Golden-leaf Ginkgo biloba Seedlings under Salinity Treatment[J].JOURNAL OF NORTHWEST FORESTRY UNIVERSITY,2020,35(2):64-71.[doi:10.3969/j.issn.1001-7461.2020.02.09]
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 NaCl处理下茉莉酸甲酯对金叶银杏光合色素、抗氧化性及黄酮代谢的调控作用()
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《西北林学院学报》[ISSN:1001-7461/CN:61-1202/S]

卷:
第35卷
期数:
2020年第2期
页码:
64-71
栏目:
出版日期:
2020-03-31

文章信息/Info

Title:
 The Regulation of Methyl Jasmonate on Photosynthetic Pigments,Antioxidation and Flavonoid Metabolism in Golden-leaf Ginkgo biloba Seedlings under Salinity Treatment
文章编号:
1001-7461(2020)02-0064-08
作者:
 袁斌玲1; 2; 王瑞敏1; 2; 陈颖1; 2*; 张涛1; 2; 陈燕琼1; 2; 沈瑒1; 2
 （1.南京林业大学 南方现代林业协同创新中心，江苏 南京 210037；2.南京林业大学 生物与环境学院，江苏 南京 210037）
Author(s):
 YUAN Bin-ling1; 2; WANG Rui-min1; 2; CHEN Ying1; 2*; ZHANG Tao1; 2; CHEN Yan-qiong1; 2; SHEN Yang1; 2
 （1.Collaborative Innovation Center of the Southern Modern Forestry,Nanjing Forestry University,Nanjing 210037,Jiangsu,China; 2.College of Biology and Environment,Nanjing Forestry University，Nanjing 210037,Jiangsu,China）
关键词:
 ; 金叶银杏; NaCl处理; 茉莉酸甲酯(MJ)调控; 生理响应; 黄酮代谢
Keywords:
 ; golden-leaf Ginkgo biloba;  NaCl treatment;  methyl jasmonate regulation;  physiological response;  flavonoid metabolism
分类号:
S718.43
DOI:
10.3969/j.issn.1001-7461.2020.02.09
文献标志码:
A
摘要:
 以金叶银杏幼苗为试验材料，通过0、50（低盐）、150（高盐）mmol·L-1 NaCl以及在50、150 mmol·L-1 NaCl下分别添加500 μmol·L-1茉莉酸甲酯（MJ） 处理金叶银杏幼苗，研究MJ在NaCl处理下金叶银杏幼苗的生理响应及对黄酮苷元含量的调控作用。结果表明，2个水平盐处理（50、150 mmol·L-1 NaCl) 及添加MJ处理后叶绿素含量降低，但低水平盐可促进类胡萝卜素的积累；低盐+MJ处理可提高叶绿素b的含量。高盐处理可显著地提高丙二醛（MDA）含量、超氧歧化酶（SOD） 活性；高盐+MJ能够显著地降低MDA含量、提高SOD酶活性，而低盐或低盐+MJ处理上述指标变化不显著。2个水平盐处理下苯丙氨酸解氨酶（PAL）、4-香豆酸辅酶A连接酶（4CL）以及肉桂醇脱氢酶（CAD）活性均有所提高，但MJ的添加并没有显著提高4CL和CAD的活性。低浓度盐处理在短期内（5 d）就可显著提高金叶银杏黄酮苷元和总黄酮的含量（比对照提高了133.7%）但低盐+MJ处理对黄酮苷元和总黄酮含量提高程度不大；高盐+MJ处理可在长时间内（10~15 d）显著提高槲皮素(154.5%)和总黄酮的含量(132.1%)。本研究为盐胁迫、茉莉酸及黄酮代谢间的作用机制及银杏黄酮的代谢调控提供一定的理论基础和技术支持。
Abstract:
 In this study,seedlings of a Ginkgo biloba cultivar “Golden-leaf” were treated by different concentrations of sodium chloride (50 mmol·L-1,low level salinity group,LS; 150 mmol·L-1,high level salinity group,HS) and combinations of NaCl and 500 μmol·L-1 methyl jasmonate (MJ) (LS+MJ,HS+MJ) to examine the physiological responses of the seedlings and regulation functions of the additives to the contents of leaf flovonoid,a group of bioactive components in the leaves of G.biloba.The results showed that the chlorophyll contents were lower than that of the control group under the two levels of salt treatments and NaCl+MJ treatments,however,the carotenoid content increased under the LS treatment,and the content of chlorophyll b increased under the LS+MJ treatment.The content of malondialdehyde (MDA),superoxide dismutase (SOD) activity increased under HS treatment.The HS+MJ treatment could significantly reduce the MDA content,improve the SOD activity，while the LS+MJ treatment had no significant effects on the above involved parameters.The activities of phenylalanine ammonialyase (PAL),4-coumarate coenzyme A ligase (4CL) and the cinnamom-alcohol dehydrogenase (CAD) were all improved under two levels of salt treatment,however,the application of MJ did not significantly improve the activities of 4CL and CAD under HS treatment.The LS treatment could significantly increase the content of flavonoid glycosides and total flavonoids (133.7% higher than that in the control) in a shorter salt treatment period (5 days).However,the HS+MJ treatment could significantly increase the contents of flavonoid glycosides (154.5% higher) and total flavonoids 132.1% higher) in a longer treatment period (10-15 d).This study would provide a theoretical basis for researching the correlation of salinity,jasmonate and flavonoid metabolism and also provide technical support for the precise regulation of flavonoid metabolism in Ginkgo leaves.

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备注/Memo

备注/Memo:
 收稿日期：2019-04-19修回日期：2019-07-13
基金项目：国家自然科学基金（31770674）；国家“十三五”重点研发计划课题（2017YFD0601301）；江苏省高校优势学科建设工程资助项目（PAPD）。
作者简介：袁斌玲，女，硕士在读，研究方向：植物抗性生理。E-mail:303769024@qq.com
*通信作者：陈颖，女，教授，研究方向：植物资源与逆境生理。E-mail:chynjfu@163.com
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