[1]章亮,谢祥琛,王奕然,等. 侧柏茎段培养快速繁殖技术研究和黄帝手植柏克隆[J].西北林学院学报,2022,37(1):112-118.[doi:10.3969/j.issn.1001-7461.2022.01.16]
　ZHANG Liang,XIE Xiang-chen,WANG Yi-ran,et al. Rapid Propagation of Platycladus orientalis by Stem Segment Culture and Clone of the P.orientalis Tree Planted by Huangdi[J].JOURNAL OF NORTHWEST FORESTRY UNIVERSITY,2022,37(1):112-118.[doi:10.3969/j.issn.1001-7461.2022.01.16]
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 侧柏茎段培养快速繁殖技术研究和黄帝手植柏克隆()
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《西北林学院学报》[ISSN:1001-7461/CN:61-1202/S]

卷:
第37卷
期数:
2022年第1期
页码:
112-118
栏目:
出版日期:
2022-01-31

文章信息/Info

Title:
 Rapid Propagation of Platycladus orientalis by Stem Segment Culture and Clone of the P.orientalis Tree Planted by Huangdi
文章编号:
1001-7461(2022)01-0112-07
作者:
 章亮; 谢祥琛; 王奕然; 李周岐*
 （西北农林科技大学 林学院，陕西 杨陵 712100）
Author(s):
 ZHANG Liang; XIE Xiang-chen; WANG Yi-ran; LI Zhou-qi*
 (College of Forestry,Northwest A&F University,Yangling 712100,Shaanxi,China)
关键词:
 ; 侧柏; 黄帝手植柏; 茎段培养; 克隆繁殖
Keywords:
Platycladus orientalis;  the Platycladus orientalis tree planted by Huangdi;  stem segment culture;  clonal reproduction
分类号:
S722.37
DOI:
10.3969/j.issn.1001-7461.2022.01.16
文献标志码:
A
摘要:
 以3个不同年龄段的侧柏茎段为外植体，探讨基本培养基、植物生长调节剂、大量元素对侧柏茎段培养快繁的影响，研究侧柏茎段培养快速繁殖技术，并分析年龄对培养各阶段的影响，进而建立了黄帝手植柏克隆繁殖技术体系。结果表明，在试验范围内，各年龄段的侧柏茎段消毒效果均随消毒时间延长而提升，黄帝手植柏最佳升汞消毒时间为8 min；各年龄段的侧柏外植体在不同培养基上启动率均大于90%，选择MS+0.1 mg·L-1 NAA+0.1 mg·L-1 6-BA作为黄帝手植柏适宜启动培养基，启动率可达100%；50 a侧柏适宜在较高激素浓度的增殖培养基上培养，800、3 000 a侧柏适宜在中等激素水平的培养基上培养，黄帝手植柏适宜增殖培养基为MS+0.1 mg·L-1 NAA+0.2 mg·L-1 6-BA，30 d增殖系数为2.52；随着侧柏年龄增大，茎段生根更加困难，800、3 000 a侧柏在培养基MS+0.1 mg·L-1 IBA+0.1 mg·L-1 6-BA+0.1 mg·L-1 NAA上有较好的生根效果，黄帝手植柏适宜生根培养基为MS+0.1 mg·L-1 IBA+0.1 mg·L-1 6-BA+0.1 mg·L-1 NAA。本研究初步建立了黄帝手植柏茎段培养技术体系，为黄帝手植柏的克隆和种质资源保存奠定了技术基础。
Abstract:
 Stem segments of three different-aged Platycladus orientalis trees were used as explants to study the effects of basic medium,hormone and macroelement on stem segment culture.The rapid propagation technology was studied,and the influences of tree age on each stage of culture were analyzed.Furthermore,the clonal propagation technology of the P.orientalis tree planted by Huangdi was established.The results showed that under the conditions of the test,the disinfection effect of all aged segments was improved with the prolongation of disinfection time,and the optimal disinfection time was 8 min.The activation rate of all the explants on different media reached more than 90%.MS+0.1 mg·L-1 NAA+0.1 mg·L-1 6-BA was selected as the suitable starting medium,and the starting rate could reach 100%.Segments of 50-year-old tree was suitable for culturing on the proliferation medium with higher hormone concentration,while the segments of 800-and 3 000-year-old tree were suitable for culturing on the medium with moderate hormone-level.The optimal medium for proliferation culture of the P.orientalis tree planted by Huangdi was MS+0.1 mg·L-1 NAA+0.2 mg·L-1 6-BA,from which the proliferation factor was 2.52 in 30 days.With the increase of the tree age,the segments were more difficult to root.The rooting effects of 800-and 3 000-year-old tree were better on the medium MS+0.1 mg·L-1 IBA+0.1 mg·L-1 6-BA+0.1 mg·L-1 NAA,which was also the optimal medium for rooting of the P.orientalis tree planted by Huangdi.The technical system for culturing the segment of the P.orientalis tree planted by Huangdi was initially established,which laid a technical foundation for the cloning and germplasm resource preservation of this most precious tree in China.

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[1]田 丽,王进鑫＊,庞云龙.不同供水条件下气象因素对侧柏和刺槐叶水势的影响[J].西北林学院学报,2008,23(03):25.
　TI AN li,WAN G Ji nxi n,PAN G Yunlong.Effect of Meteorological Factors on Leaf Water Potential of Platycla dusorientali sand Robinia pseudoacacia under Different Water Rejimes[J].JOURNAL OF NORTHWEST FORESTRY UNIVERSITY,2008,23(1):25.
[2]吉增宝,王进鑫.干旱胁迫对侧柏幼树某些生理特性的影响[J].西北林学院学报,2009,24(06):6.
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　WANG Di-hai,ZHAO Zhong,ZHANG Yan. The Fine Root of Robinia pseudoacacia and Soil Moisture in the Loess Plateau[J].JOURNAL OF NORTHWEST FORESTRY UNIVERSITY,2012,27(1):1.
[5]耿生莲. 不同土壤水分下黑果枸杞生理特点分析[J].西北林学院学报,2012,27(01):6.
　GENG Sheng lian. Physio Characteristics of Lycium ruthenicum under Different Soil Water Conditions[J].JOURNAL OF NORTHWEST FORESTRY UNIVERSITY,2012,27(1):6.
[6]乌凤章. 辽南地区越橘根系分布特征[J].西北林学院学报,2012,27(01):11.
　WU Feng zhang. Characteristics of Root System of Blueberry in South of Liaoning Province[J].JOURNAL OF NORTHWEST FORESTRY UNIVERSITY,2012,27(1):11.
[7]张学星,周筑,陈强,等. 17种云南乡土树种对不同程度水涝胁迫的忍耐性调查[J].西北林学院学报,2012,27(01):15.
　ZHANG Xue xing,ZHOU Zhu,CHEN Qiang,et al. Waterlogging Tolerance of 17 Native Tree Species in Yunnan[J].JOURNAL OF NORTHWEST FORESTRY UNIVERSITY,2012,27(1):15.
[8]钱瑭璜,雷江丽,庄雪影. 3种草本蕨类植物耐旱性研究[J].西北林学院学报,2012,27(01):22.
　QIAN Tang-huang,LEI Jiang-li,ZHUANG Xue-ying. Anti-drought Stress Capacity of 3 Herbal Ferns[J].JOURNAL OF NORTHWEST FORESTRY UNIVERSITY,2012,27(1):22.
[9]彭邵锋,王瑞,陈永忠,等. 油茶无性系果实生长期光合特性研究[J].西北林学院学报,2012,27(01):28.
　PENG Shao-feng,WANG Rui,CHEN Yong-zhong,et al. Photosynthetic Characteristics in Fruit-growing Stage of Camellia oleifera Clones[J].JOURNAL OF NORTHWEST FORESTRY UNIVERSITY,2012,27(1):28.
[10]李博,陶功胜,谢寅峰,等. 纳米TiO2对髯毛箬竹光合作用日变化的影响[J].西北林学院学报,2012,27(01):35.
　LI Bo,TAO Gong-sheng,XIE Yin-feng,et al. Effects of Nanometer TiO2 on Diurnal Variation of Photosynthesis of Indocalamus barbatus McClure[J].JOURNAL OF NORTHWEST FORESTRY UNIVERSITY,2012,27(1):35.
[11]初江涛,王进鑫,邹朋,等. 干旱和铅胁迫对生长初期的国槐和侧柏叶绿素的影响[J].西北林学院学报,2012,27(04):19.
　CHU Jiang-tao,WANG Jin-xin,ZOU Peng,et al. Effects of Drought and Pb Stress on the Contents of Chlorophylls of Sophor japonica and Platycladus orientalis in Their Initial Growth Stages[J].JOURNAL OF NORTHWEST FORESTRY UNIVERSITY,2012,27(1):19.
[12]刘俊峰,王进鑫*,邹朋,等. 水分和铅交互胁迫对侧柏幼苗叶片生理指标的影响[J].西北林学院学报,2014,29(02):32.[doi:doi:10.3969/j.issn.1001-7461.2014.02.06]
　LIU Jun-feng,WANG Jin-xin*,ZOU Peng,et al. Effects of Interactive Drought and Lead Stress on Physiological Index of Platycladus orientalis Seedlings[J].JOURNAL OF NORTHWEST FORESTRY UNIVERSITY,2014,29(1):32.[doi:doi:10.3969/j.issn.1001-7461.2014.02.06]
[13]王艳,王迪海*,张剑南,等. 黄土区不同密度侧柏人工林树冠二维特征的差异[J].西北林学院学报,2014,29(03):125.[doi:doi:10.3969/j.issn.1001-7461.2014.03.25]
　WANG Yan,WANG Di-hai*,ZHANG Jian-nan,et al. Differences of Crown Two-dimensional Features of Platycladus orientalis Plantation under Different Stand Densities in Loess Area[J].JOURNAL OF NORTHWEST FORESTRY UNIVERSITY,2014,29(1):125.[doi:doi:10.3969/j.issn.1001-7461.2014.03.25]
[14]李春燕,王进鑫*,薛设. 干旱及复水对侧柏幼苗叶片抗氧化酶活性的影响[J].西北林学院学报,2015,30(02):33.[doi:doi:10.3969/j.issn.1001-7461.2015.02.06]
　LI Chun-yan,WANG Jin-xin*,XUE She. Effect of Drought and Rewatering on the Activity of Protect Enzymes in the Leaves of Platycladus orientalis Seedings[J].JOURNAL OF NORTHWEST FORESTRY UNIVERSITY,2015,30(1):33.[doi:doi:10.3969/j.issn.1001-7461.2015.02.06]
[15]陈科皓,王进鑫*,李继文. 不同生长期旱后复水对侧柏幼苗生物量的影响[J].西北林学院学报,2015,30(02):38.[doi:doi:10.3969/j.issn.1001-7461.2015.02.07]
　CHEN Ke-hao,WANG Jin-xin*,LI Ji-wen. Effect of Re-watering after Drought on Biomass of Platycladus orientalis Seedlings in Different Growth Stages[J].JOURNAL OF NORTHWEST FORESTRY UNIVERSITY,2015,30(1):38.[doi:doi:10.3969/j.issn.1001-7461.2015.02.07]
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　WANG Min,WANG Jin-xin*,LI Ji-wen. Effects of Growth Period, Drought and Rewater on the UWL of Seedling Leaves[J].JOURNAL OF NORTHWEST FORESTRY UNIVERSITY,2015,30(1):39.[doi:doi:10.3969/j.issn.1001-7461.2015.03.07]
[17]冯志培,赵佳宝,孔玉华,等. 种基盘对侧柏幼苗根系形态和生理特性的影响[J].西北林学院学报,2015,30(03):107.[doi:doi:10.3969/j.issn.1001-7461.2015.03.19]
　FENG Zhi-pei,ZHAO Jia-bao,KONG Yu-hua,et al. Effect of Seed-base Technique on the Root Morphological and Physiological Properties of Platycladus orientalis[J].JOURNAL OF NORTHWEST FORESTRY UNIVERSITY,2015,30(1):107.[doi:doi:10.3969/j.issn.1001-7461.2015.03.19]
[18]李健康,王培新,孟惠荣,等. 2种植物抗逆剂对苗木抗旱促长作用研究[J].西北林学院学报,2015,30(05):111.[doi:doi:10.3969/j.issn.1001-7461.2015.05.18]
　LI Jian-kang,WANG Pei-xin,MENG Hui-rong,et al. Promotion Effects of Two Plant Anti-adversity Agents on Seedling Growth[J].JOURNAL OF NORTHWEST FORESTRY UNIVERSITY,2015,30(1):111.[doi:doi:10.3969/j.issn.1001-7461.2015.05.18]
[19]孔玉华,王怡博,赵佳宝,等. 侧柏、山毛桃单栽及侧柏-山毛桃混栽幼苗根际土壤环境特征[J].西北林学院学报,2016,31(2):72.[doi:10.3969/j.issn.1001-7461.2016.02.13]
　KONG Yu-hua,WANG Yi-bo,ZHAO Jia-bao,et al. Rhizospheric Soil Environmental Characteristics under Pure and Mixed Plantations of Platycladus orientalis and Prunus davidiana Seedlings[J].JOURNAL OF NORTHWEST FORESTRY UNIVERSITY,2016,31(1):72.[doi:10.3969/j.issn.1001-7461.2016.02.13]
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　NI Yan-yan,CHANG Er-mei,LIU Jian-feng*,et al. Comparison on Photosynthetic Physiology in Various Age Scions of Platycladus orientalis[J].JOURNAL OF NORTHWEST FORESTRY UNIVERSITY,2017,32(1):19.[doi:10.3969/j.issn.1001-7461.2017.01.03]

备注/Memo

备注/Memo:
 收稿日期：2021-02-26修回日期：2021-04-02
基金项目：陕西省林业厅项目（K332021313）。
第一作者：章亮。研究方向：林木遗传育种。E-mail：576611239@qq.com
*通信作者：李周岐，教授，博士生导师。研究方向：林木遗传育种。E-mail：lizhouqi@nwsuaf.edu.cn
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