[1]虞家昕,战昊,王思进,等. 防褐化处理对蒙古栎插穗生根及其生理生化的影响[J].西北林学院学报,2022,37(1):119-124.[doi:10.3969/j.issn.1001-7461.2022.01.17]
　YU Jia-xin,ZHAN Hao,WANG Si-jin,et al. Effects of the Anti-Browning Treatment on the Cutting Rooting of Quercus mongolica and Their Physiology and Biochemistry[J].JOURNAL OF NORTHWEST FORESTRY UNIVERSITY,2022,37(1):119-124.[doi:10.3969/j.issn.1001-7461.2022.01.17]
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 防褐化处理对蒙古栎插穗生根及其生理生化的影响()
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《西北林学院学报》[ISSN:1001-7461/CN:61-1202/S]

卷:
第37卷
期数:
2022年第1期
页码:
119-124
栏目:
出版日期:
2022-01-31

文章信息/Info

Title:
 Effects of the Anti-Browning Treatment on the Cutting Rooting of Quercus mongolica and Their Physiology and Biochemistry
文章编号:
1001-7461(2022)01-0119-06
作者:
 虞家昕; 战昊; 王思进; 王天宠; 陆秀君*
 （沈阳农业大学 林学院，辽宁 沈阳 110866）
Author(s):
 YU Jia-xin; ZHAN Hao; WANG Si-jin; WANG Tian-chong; LU Xiu-jun*
 （College of Forestry,Shenyang Agricultural University,Shenyang 110866,Liaoning,China）
关键词:
 ; 蒙古栎; 嫩枝扦插; 防褐化; 氧化蛋白酶活性; 内源激素
Keywords:
Quercus mongolica;  twig cutting;  anti-browning;  oxidative protease activity;  endogenous hormone
分类号:
S723.1
DOI:
10.3969/j.issn.1001-7461.2022.01.17
文献标志码:
A
摘要:
 研究防褐化处理对蒙古栎嫩枝扦插生根的影响。以蒙古栎3年实生苗的嫩枝为材料，分别采用乙醇、硝酸银、抗坏血酸对插穗进行防褐化处理，采用500 mg·L-1 IBA-K浸蘸处理后扦插，并分别在扦插后0、10、20、30、40、50 d取样测定不同生根时期氧化蛋白酶活性和内源激素含量。结果表明，防褐化处理可有效提高扦插生根率，以1%乙醇1 h+500 mg·L-1 IBA-K处理2 h最佳，生根率、根系效果指数分别为21.1%和5.713。在生根过程中，外施500 mg·L-1 IBA-K能够提升插穗内POD、PPO的活性，降低IAAO的活性，且IAA/ABA、IAA/ZR的比值升高，有利于蒙古栎嫩枝扦插生根。由此说明1%乙醇1 h浸泡结合500 mg·L-1 IBA-K激素处理，通过提高POD与PPO的活性，可降低IAAO的活性，增加IAA/ZR和IAA/ABA的比值，提升蒙古栎嫩枝扦插成活率。
Abstract:
 The effects of the anti-browning treatment on the cutting rooting of Quercus mongolica were studied.The twigs of the 3-year-old seedlings of Q.mongolica were used as materials.Ethanol,silver nitrate,and ascorbic acid were used to conduct the anti-browning treatment,and the cutting were carried out after the dip treatment with 500 mg/L indole-3-butyric acid potassium salt (IBA-K).Meanwhile,samples were taken at 0,10,20,30,40,and 50 days after cutting to determine the oxidative protease activity and endogenous hormone content at different rooting stages.The anti-browning treatment could effectively increase the rooting rate of the cuttings.Among them,the treatment with 1% ethanol for 1h and 500 mg/L IBA-K for 2 h was the best.The rooting rate and the root effect index were 21.1% and 5.713,respectively.During the rooting process,the external application of 500 mg/L IBA-K could increase the activity of POD and PPO in the cuttings,reduce the activity of IAAO,and increase the ratio of IAA/ABA and IAA/ZR,which were beneficial to cuttings.By means of increasing the activity of POD and PPO,dipping with 1% ethanol for 1 h in combination with 500 mg/L IBA-K hormone treatment can reduce the activity of IAAO,increase the ratio of IAA/ZR and IAA/ABA,and improve the cutting survival rate.

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备注/Memo

备注/Memo:
 收稿日期：2021-03-12修回日期：2021-04-24
基金项目：国家重点研发计划课题（2017YFD0600602）。
第一作者：虞家昕。研究方向：森林培育。E-mail：yujiaxinshenyang@foxmail.com
*通信作者：陆秀君，教授，博士生导师。研究方向：森林培育。E-mail：lxjsyau@syau.edu.cn
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