[1]张恒,何静*,张小彦,等. 氟唑菌酰羟胺与咪鲜胺复配对枸杞根腐病菌的毒力增效作用[J].西北林学院学报,2022,37(1):152-158.[doi:10.3969/j.issn.1001-7461.2022.01.22]
　ZHANG Heng,HE Jing*,ZHANG Xiao-yan,et al. Virulence Synergism of Pydiflumetofen and Prochloraz Complex to Chinese Wolfberry Root Rot[J].JOURNAL OF NORTHWEST FORESTRY UNIVERSITY,2022,37(1):152-158.[doi:10.3969/j.issn.1001-7461.2022.01.22]
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 氟唑菌酰羟胺与咪鲜胺复配对枸杞根腐病菌的毒力增效作用()
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《西北林学院学报》[ISSN:1001-7461/CN:61-1202/S]

卷:
第37卷
期数:
2022年第1期
页码:
152-158
栏目:
出版日期:
2022-01-31

文章信息/Info

Title:
 Virulence Synergism of Pydiflumetofen and Prochloraz Complex to Chinese Wolfberry Root Rot
文章编号:
1001-7461(2022)01-0152-07
作者:
 张恒1; 何静1*; 张小彦1; 张树衡1; 李彦湘1; 张金花1; 程永2; 李红磊2
 （1.甘肃农业大学 林学院，甘肃 兰州 730070；2.先正达（中国）投资有限公司，上海 200120）
Author(s):
 ZHANG Heng1; HE Jing1*; ZHANG Xiao-yan1; ZHANG Shu-heng1; LI Yan-xiang1; ZHANG Jin-hua1; CHENG Yong2; LI Hong-lei2
 (1.College of Forestry,Gansu Agricultural University,Lanzhou 730070,Gansu,China; 2.Syngenta (China) Investment Co.Ltd.,Shanghai 200120,China)
关键词:
 ; 杀菌剂; 复配; 枸杞根腐病; 腐皮镰刀菌; 毒力测定
Keywords:
 ; fungicide;  compound;  Chinese wolfberry root rot;  Fusarium solani;  toxicity test
分类号:
S767.1
DOI:
10.3969/j.issn.1001-7461.2022.01.22
文献标志码:
A
摘要:
 为探索氟唑菌酰羟胺与咪鲜胺复配对枸杞根腐病主要病原菌——腐皮镰刀菌的毒力增效作用，采用菌丝生长速率法，测定氟唑菌酰羟胺与咪鲜胺17种不同比例的复配处理对腐皮镰刀菌的室内毒力作用，在最佳增效配比下，明确复配处理相较单剂对病原菌孢子萌发、产孢量、芽管伸长和菌丝干重的影响。结果表明，氟唑菌酰羟胺与咪鲜胺单剂及其复配处理对腐皮镰刀菌的菌丝生长抑制效果均有明显作用，其单剂EC50值分别为0.365 8 mg·L-1和0.120 8 mg·L-1，氟唑菌酰羟胺与咪鲜胺的配比在3∶2时增效作用最大，EC50值为0.044 3 mg·L-1，增效系数SR达4.560 0，可作为枸杞根腐病药剂防治的最佳复配处理；氟唑菌酰羟胺与咪鲜胺单剂及其复配处理对腐皮镰刀菌的孢子萌发均具有一定的抑制效果，其单剂EC50值分别为0.116 7 mg·L-1和1 455.784 8 mg·L-1，最佳复配处理EC50值为0.060 2 mg·L-1，增效系数SR达3.231 1。在质量浓度为0.8 mg·L-1时，最佳复配处理对腐皮镰刀菌产孢量的抑制率达100%，对芽管伸长、菌丝干重的抑制效果均显著高于单剂处理。因此，氟唑菌酰羟胺与咪鲜胺复配比在3∶2时，可作为枸杞根腐病药剂防治的最佳复配处理，为预防耐药性的产生提供了解决途径。
Abstract:
 The objective of this study was to explore the effect of pydiflumetofen and prochloraz complex on the main pathogen of wolfberry root rot-Fusarium solani.The indoor toxicity of 17 different proportions of pydiflumetofen and prochloraz to F.solani was determined by mycelial growth rate method.Under the optimal synergistic ratio,the effects of compound treatment on spore germination,spore production,germ tube elongation and mycelial dry weight of F.solani were clarified and compared with single agent.The results showed that the single agent of pydiflumetofen and prochloraz and their compound treatment had significant inhibitory effects on the mycelial growth of F.solani.The EC50 values of single agent were 0.365 8 (pydiflumetofen) and 0.120 8 mg·L-1 (prochloraz),respectively.The synergistic effect was the highest when the ratio of pydiflumetofen and prochloraz was 3∶2,the EC50 value was 0.044 3 mg·L-1 and the synergistic coefficient SR reached 4.560 0,which could be used as the best compound treatment for the control of wolfberry root rot.The single agent of pydiflumetofen and prochloraz and their compound treatment had certain inhibitory effects on the spore germination of F.solani.The EC50 values of single agent were 0.116 7 and 1 455.7848 mg·L-1,respectively.The EC50 value of the best compound treatment was 0.060 2 mg·L-1,and the synergistic coefficient SR reached 3.231 1.When the concentration was 0.8 mg·L-1,the inhibition rate of the best compound treatment on the spore production of F.solani was 100%,and the inhibition effects on the elongation of germ tube and dry weight of mycelium were significantly higher than those of single agent.Therefore,when the ratio of pydiflumetofen and prochloraz was 3∶2,it could be used as the best compound treatment for the control of wolfberry root rot,which provided a solution for the prevention of drug resistance.

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备注/Memo

备注/Memo:
 收稿日期：2021-02-27修回日期：2021-03-11
基金项目：国家自然科学基金地区基金（32060341）；甘肃省自然基金（20JR10RA508）；甘肃农业大学青年导师扶持基金（GAU-QDFC-2018-07）。
第一作者：张恒。研究方向：森林保护。E-mail:1075904206@qq.com
*通信作者：何静，博士，副教授。研究方向：林业有害生物防治。E-mail:hejing268@aliyun.com
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