[1]王如月,王雪菱,李际红*,等. 美洲黑杨杂交良种‘鲁林9号杨’离体再生体系的建立[J].西北林学院学报,2022,37(2):83-89.[doi:10.3969/j.issn.1001-7461.2022.02.11]
　WANG Ru-yue,WANG Xue-ling,LI Ji-hong*,et al. Establishment of in vitro Regeneration System of Populus deltoides×’Lulin No.9’[J].JOURNAL OF NORTHWEST FORESTRY UNIVERSITY,2022,37(2):83-89.[doi:10.3969/j.issn.1001-7461.2022.02.11]
点击复制

 美洲黑杨杂交良种‘鲁林9号杨’离体再生体系的建立()
分享到：

《西北林学院学报》[ISSN:1001-7461/CN:61-1202/S]

卷:
第37卷
期数:
2022年第2期
页码:
83-89
栏目:
出版日期:
2022-03-31

文章信息/Info

Title:
 Establishment of in vitro Regeneration System of Populus deltoides×’Lulin No.9’
文章编号:
1001-7461(2022)02-0083-07
作者:
 王如月; 王雪菱; 李际红*; 王冬月; 牛牧歌
 （山东农业大学 林学院，山东 泰安 271018）
Author(s):
 WANG Ru-yue; WANG Xue-ling; LI Ji-hong*; WANG Dong-yue; NIU Mu-ge
 (College of Forestry,Shandong Agricultural University,Tai’an,Shandong 271018,China)
关键词:
 ; ‘鲁林9号杨’; 组织培养; 再生体系
Keywords:
Populus deltoides× ’Lulin 9’;  tissue culture;  regeneration system
分类号:
S792.11
DOI:
10.3969/j.issn.1001-7461.2022.02.11
文献标志码:
A
摘要:
 以‘鲁林9号杨’无菌叶片与叶柄作为外植体，建立‘鲁林9号杨’的离体再生体系。结果表明，叶片诱导不定芽的最佳培养基为1/2 MS+0.3 mg·L-16-BA+0.15 mg·L-1NAA，诱导出芽率为100%，平均出芽数为10.83个；叶柄诱导不定芽的最佳培养基为1/2 MS+0.5 mg·L-16-BA+0.1 mg·L-1NAA，诱导出芽率为94%，平均出芽数为5.67个；不定芽生根的最佳培养基为1/2MS+0.15 mg·L-1IBA+0.03 mg·L-1NAA，其生根率为95.2%，平均每株生根5条。组培苗炼苗移栽成活率达95%。该研究旨在建立‘鲁林9号杨’的离体再生体系，为‘鲁林9号杨’品种遗传改良和分子生物学研究等提供试验基础，为培育高品质、速生、高抗的杨树新品种奠定理论基础。
Abstract:
 In this study,the sterile leaves and petioles of Populus deltoides×’Lulin No.9’ were used to produce adventitious buds,then the adventitious buds were rooted to form regenerated plants.The results showed that the optimum medium of adventitious buds induced by the leaves of P.deltoides×’Lulin No.9’ was 1/2 MS+0.3 mg·L-16-BA+0.15 mg·L-1NAA,the induction rate of adventitious bud was 100%,and the average number of buds was 10.83.The optimum medium for inducing adventitious buds from petioles was 1/2 MS+0.5 mg·L-16-BA+0.1 mg·L-1NAA,the induction rate of adventitious bud was 94%,and the average number of buds was 5.67.The best rooting medium was 1/2MS+0.15 mg·L-1IBA+0.03 mg·L-1NAA.The rooting rate was 95.2%,with an average of 5 roots per plant.The survival rate of the regenerated seedlings was 95%.The purpose of this study was to establish an in vitro regeneration system of P.deltoides×’Lulin No.9’,to provide an experimental basis for genetic improvement and molecular biology research of P.deltoides×’Lulin 9’,and to lay a theoretical foundation for cultivating new poplar varieties with high quality,fast growth and high resistance.

相似文献/References:

[1]李亚藏,梁彦兰,王庆成. 铅对山梨和山荆子光合作用和叶绿素荧光特性的影响[J].西北林学院学报,2012,27(05):21.
　LI Ya-cang,LIANG Yan-lan,WANG Qing-cheng. Influence of Pb on Photosynthesis and Chlorophyll Fluorescence Characteristics in Pyrus ussuriensis and Malus baccata [J].JOURNAL OF NORTHWEST FORESTRY UNIVERSITY,2012,27(2):21.
[2]张小咏,李佳,杨艳昭,等. 基于SWAT模型的长江源区径流模拟[J].西北林学院学报,2012,27(05):38.
　ZHANG Xiao-yong,LI Jia,YANG Yan-zhao,et al. Runoff Simulation of the Catchment of the Headwaters of the Yangtze River Based on SWAT Model[J].JOURNAL OF NORTHWEST FORESTRY UNIVERSITY,2012,27(2):38.
[3]杨红旗,陈广辉,王金林. PVAc改性酚醛树脂制备铝木复合材料研究[J].西北林学院学报,2013,28(01):170.[doi:10.3969/j.issn.1001-7461.2013.01.34]
　YANG Hong-qi,CHEN Guang-hui,WANG Jin-lin. PF Adhesive Modified by PVAc for Manufacturing Technology of Decorative Fiberboard with Aluminum Foils[J].JOURNAL OF NORTHWEST FORESTRY UNIVERSITY,2013,28(2):170.[doi:10.3969/j.issn.1001-7461.2013.01.34]
[4]江涛,李秀荣,谢延军,等. 微波预处理对巨尾桉木材渗透性的影响[J].西北林学院学报,2013,28(01):174.[doi:10.3969/j.issn.1001-7461.2013.01.35]
　JIANG Tao,LI Xiu-rong,XIE Yan-jun,et al. Effects of Microwave Pretreatment on the Permeability of Eucalyptus grandis×Eucalyptus urophylla[J].JOURNAL OF NORTHWEST FORESTRY UNIVERSITY,2013,28(2):174.[doi:10.3969/j.issn.1001-7461.2013.01.35]
[5]牛晓霆,王逢瑚,曹新民. 明清家具匠师原木下料的工艺原则及措施[J].西北林学院学报,2013,28(01):178.[doi:10.3969/j.issn.1001-7461.2013.01.36]
　NIU Xiao-ting,WANG Feng-hu,CAO Xin-min. Techniques and Principles of Log Cuttings of Furniture Makers in Ming and Qing Dynasties[J].JOURNAL OF NORTHWEST FORESTRY UNIVERSITY,2013,28(2):178.[doi:10.3969/j.issn.1001-7461.2013.01.36]
[6]吕金阳,覃卓凯,舒辉,等. 银杉木材构造美学价值[J].西北林学院学报,2013,28(01):183.[doi:10.3969/j.issn.1001-7461.2013.01.37]
　LV Jin-yang,QIN Zhuo-kai,SHU Hui,et al. Esthetical Values in Wood Structure of Cathaya argyrophylla[J].JOURNAL OF NORTHWEST FORESTRY UNIVERSITY,2013,28(2):183.[doi:10.3969/j.issn.1001-7461.2013.01.37]
[7]杨艳红,张海敏,孙景荣. 基于传统文化视角的现代家具设计探析[J].西北林学院学报,2013,28(01):188.[doi:10.3969/j.issn.1001-7461.2013.01.38]
　YANG Yan-hong,ZHANG Hai-min,SUN Jing-rong. Study of Modern Furniture Design Based on Traditional Culture[J].JOURNAL OF NORTHWEST FORESTRY UNIVERSITY,2013,28(2):188.[doi:10.3969/j.issn.1001-7461.2013.01.38]
[8]黄圣游,叶喜. 新中式家具“中式”传统的界定[J].西北林学院学报,2013,28(01):192.[doi:10.3969/j.issn.1001-7461.2013.01.39]
　HUANG Sheng-You,YE Xi. Definition of Traditional Element in New Chinese Style Furniture[J].JOURNAL OF NORTHWEST FORESTRY UNIVERSITY,2013,28(2):192.[doi:10.3969/j.issn.1001-7461.2013.01.39]
[9]韩维生,赵明磊,王宏斌. 基于阈限理论的设计尺度体系[J].西北林学院学报,2013,28(01):197.[doi:10.3969/j.issn.1001-7461.2013.01.40]
　HAN Wei-sheng,ZHAO Ming-lei,WANG Hong-bin. Design Scale System Based on Threshold Theory[J].JOURNAL OF NORTHWEST FORESTRY UNIVERSITY,2013,28(2):197.[doi:10.3969/j.issn.1001-7461.2013.01.40]
[10]宋杰,侯建军,申黎明. 按摩椅按摩头包覆层对按摩舒适性的影响[J].西北林学院学报,2013,28(01):202.[doi:10.3969/j.issn.1001-7461.2013.01.41]
　SONG Jie,HOU Jian-jun,SHEN Li-ming. Influence of Massager Coat Layer of Massage Chairs on the Degree of Comfort[J].JOURNAL OF NORTHWEST FORESTRY UNIVERSITY,2013,28(2):202.[doi:10.3969/j.issn.1001-7461.2013.01.41]

备注/Memo

备注/Memo:
 收稿日期：2021-09-15修回日期：2021-10-25
基金项目：转基因生物新品种培育重大专项(2018ZX08020002)；山东省自然基金项目(ZR2013CM020)；山东省农业良种项目（201496）。
第一作者：王如月。研究方向：林木遗传学与生物技术。E-mail:1982531958@qq.com
*通信作者：李际红，博士，教授，博士生导师。研究方向：林木遗传学与生物技术。E-mail:jhli@sdau.edu.cn
更新日期/Last Update: