[1]张君,雍婧姣,路国栋,等. 利用微型离心管提取分析天然橡胶主要成分聚异戊二烯方法的建立[J].西北林学院学报,2023,38(2):223-228.[doi:10.3969/j.issn.1001-7461.2023.02.31]
　ZHANG Jun,YONG Jing-jiao,LU Guo-dong,et al. Establishment of A Microtube Extraction Method for Natural Rubber Basic Ingredient Polyisoprene Analysis[J].JOURNAL OF NORTHWEST FORESTRY UNIVERSITY,2023,38(2):223-228.[doi:10.3969/j.issn.1001-7461.2023.02.31]
点击复制

 利用微型离心管提取分析天然橡胶主要成分聚异戊二烯方法的建立()
分享到：

《西北林学院学报》[ISSN:1001-7461/CN:61-1202/S]

卷:
第38卷
期数:
2023年第2期
页码:
223-228
栏目:
出版日期:
2023-03-31

文章信息/Info

Title:
 Establishment of A Microtube Extraction Method for Natural Rubber Basic Ingredient Polyisoprene Analysis
文章编号:
1001-7461(2023)02-0223-06
作者:
 张君1; 2; 3; 雍婧姣1; 2; 3; 路国栋1; 2; 3; 安迎瑞1; 2; 3; 张虹1; 2; 3; 郎思睿1; 2; 3; 陈任1; 2; 3*
 （1.宁夏大学 西部特色生物资源保护与利用教育部重点实验室,宁夏 银川 750021；2.宁夏大学 生命科学学院,宁夏 银川 750021；3.宁夏大学 宁夏优势特色作物现代分子育种重点实验室,宁夏 银川 750021）
Author(s):
 ZHANG Jun1; 2; 3; YONG Jing-jiao1; 2; 3; LU Guo-dong1; 2; 3; AN Ying-rui1; 2; 3; ZHANG Hong1; 2; 3; LANG Si-rui1; 2; 3; CHEN Ren1; 2; 3*
 (1.Key Laboratory of Ministry of Education for Protection and Utilization of Special Biological Resources in Western China,Ningxia University,Yinchuan 750021,Ningxia,China; 2.School of Life Science,Ningxia University,Yinchuan 750021,Ningxia,China; 3.Key Laboratory of Modern Molecular Breeding for Dominant and Special Crops in Ningxia,Ningxia University,Yinchuan 750021,Ningxia,China)
关键词:
 ; 天然橡胶; 聚异戊二烯; 微型离心管提取; 索氏提取器提取; 提取得率; 分子量分布
Keywords:
 ; natural rubber;  polyisoprene;  microtube extraction method;  Soxhlet extraction method;  extraction rate;  molecular weight distribution
分类号:
S718.43
DOI:
10.3969/j.issn.1001-7461.2023.02.31
文献标志码:
A
摘要:
 为建立一种快速、准确的从少量样品中提取、分析天然橡胶主要成分聚异戊二烯含量的方法，在已采用索氏提取器提取的基础上，进一步探讨采用2 mL微型离心管的方法提取分析材料（0.5 g以下）中的聚异戊二烯含量。从分别利用微型离心管与索氏提取器提取4种样品分析材料，即顺式聚异戊二烯标准品、多汁乳菇子实体、杠柳枝条、杜仲果翅的提取得率和分子量分布看，2种提取方法的差别不大，再通过每次提取时利用顺式聚异戊二烯标准品建立标准曲线校正，采用2 mL微型离心管微量提取分析测定植物植株、组织材料或真菌子实体、菌丝体中的顺式或反式-1、4-聚异戊二烯橡胶含量的方法可行、结果可信。
Abstract:
 In order to establish a rapid,accurate analysis method for the content test of polyisoprene,the basic ingredient in natural rubber that extracted from a small quantity of sample,based on the previous method by Soxhlet extractor,the extraction and analysis of polyisoprene from sample (less than 0.5 g) in 2 mL microtube was further discussed.Four kinds of samples,cis-polyisoprene standard,Lactarius volemus fruiting body,Periploca sepium branch and Eucommia ulmoides winged perianth,were extracted by microtube and Soxhlet extractor respectively.No significant differences were found in extraction rate and molecular weight distribution between the two methods.In addition,the content of polyisoprene could be corrected by the standard curve established by cis-polyisoprene standards during each extraction.The 2 mL microtube extraction method is feasible and reliable to analyze the content of cis-or trans-1,4-polyisoprene in plant plantlet,tissue materials or fungi fruiting bodies,mycelia.

相似文献/References:

[1]李亚藏,梁彦兰,王庆成. 铅对山梨和山荆子光合作用和叶绿素荧光特性的影响[J].西北林学院学报,2012,27(05):21.
　LI Ya-cang,LIANG Yan-lan,WANG Qing-cheng. Influence of Pb on Photosynthesis and Chlorophyll Fluorescence Characteristics in Pyrus ussuriensis and Malus baccata [J].JOURNAL OF NORTHWEST FORESTRY UNIVERSITY,2012,27(2):21.
[2]张小咏,李佳,杨艳昭,等. 基于SWAT模型的长江源区径流模拟[J].西北林学院学报,2012,27(05):38.
　ZHANG Xiao-yong,LI Jia,YANG Yan-zhao,et al. Runoff Simulation of the Catchment of the Headwaters of the Yangtze River Based on SWAT Model[J].JOURNAL OF NORTHWEST FORESTRY UNIVERSITY,2012,27(2):38.
[3]杨红旗,陈广辉,王金林. PVAc改性酚醛树脂制备铝木复合材料研究[J].西北林学院学报,2013,28(01):170.[doi:10.3969/j.issn.1001-7461.2013.01.34]
　YANG Hong-qi,CHEN Guang-hui,WANG Jin-lin. PF Adhesive Modified by PVAc for Manufacturing Technology of Decorative Fiberboard with Aluminum Foils[J].JOURNAL OF NORTHWEST FORESTRY UNIVERSITY,2013,28(2):170.[doi:10.3969/j.issn.1001-7461.2013.01.34]
[4]江涛,李秀荣,谢延军,等. 微波预处理对巨尾桉木材渗透性的影响[J].西北林学院学报,2013,28(01):174.[doi:10.3969/j.issn.1001-7461.2013.01.35]
　JIANG Tao,LI Xiu-rong,XIE Yan-jun,et al. Effects of Microwave Pretreatment on the Permeability of Eucalyptus grandis×Eucalyptus urophylla[J].JOURNAL OF NORTHWEST FORESTRY UNIVERSITY,2013,28(2):174.[doi:10.3969/j.issn.1001-7461.2013.01.35]
[5]牛晓霆,王逢瑚,曹新民. 明清家具匠师原木下料的工艺原则及措施[J].西北林学院学报,2013,28(01):178.[doi:10.3969/j.issn.1001-7461.2013.01.36]
　NIU Xiao-ting,WANG Feng-hu,CAO Xin-min. Techniques and Principles of Log Cuttings of Furniture Makers in Ming and Qing Dynasties[J].JOURNAL OF NORTHWEST FORESTRY UNIVERSITY,2013,28(2):178.[doi:10.3969/j.issn.1001-7461.2013.01.36]
[6]吕金阳,覃卓凯,舒辉,等. 银杉木材构造美学价值[J].西北林学院学报,2013,28(01):183.[doi:10.3969/j.issn.1001-7461.2013.01.37]
　LV Jin-yang,QIN Zhuo-kai,SHU Hui,et al. Esthetical Values in Wood Structure of Cathaya argyrophylla[J].JOURNAL OF NORTHWEST FORESTRY UNIVERSITY,2013,28(2):183.[doi:10.3969/j.issn.1001-7461.2013.01.37]
[7]杨艳红,张海敏,孙景荣. 基于传统文化视角的现代家具设计探析[J].西北林学院学报,2013,28(01):188.[doi:10.3969/j.issn.1001-7461.2013.01.38]
　YANG Yan-hong,ZHANG Hai-min,SUN Jing-rong. Study of Modern Furniture Design Based on Traditional Culture[J].JOURNAL OF NORTHWEST FORESTRY UNIVERSITY,2013,28(2):188.[doi:10.3969/j.issn.1001-7461.2013.01.38]
[8]黄圣游,叶喜. 新中式家具“中式”传统的界定[J].西北林学院学报,2013,28(01):192.[doi:10.3969/j.issn.1001-7461.2013.01.39]
　HUANG Sheng-You,YE Xi. Definition of Traditional Element in New Chinese Style Furniture[J].JOURNAL OF NORTHWEST FORESTRY UNIVERSITY,2013,28(2):192.[doi:10.3969/j.issn.1001-7461.2013.01.39]
[9]韩维生,赵明磊,王宏斌. 基于阈限理论的设计尺度体系[J].西北林学院学报,2013,28(01):197.[doi:10.3969/j.issn.1001-7461.2013.01.40]
　HAN Wei-sheng,ZHAO Ming-lei,WANG Hong-bin. Design Scale System Based on Threshold Theory[J].JOURNAL OF NORTHWEST FORESTRY UNIVERSITY,2013,28(2):197.[doi:10.3969/j.issn.1001-7461.2013.01.40]
[10]宋杰,侯建军,申黎明. 按摩椅按摩头包覆层对按摩舒适性的影响[J].西北林学院学报,2013,28(01):202.[doi:10.3969/j.issn.1001-7461.2013.01.41]
　SONG Jie,HOU Jian-jun,SHEN Li-ming. Influence of Massager Coat Layer of Massage Chairs on the Degree of Comfort[J].JOURNAL OF NORTHWEST FORESTRY UNIVERSITY,2013,28(2):202.[doi:10.3969/j.issn.1001-7461.2013.01.41]

备注/Memo

备注/Memo:
 收稿日期：2022-08-10修回日期：2022-11-06
基金项目：国家自然科学基金目（31960065）；宁夏回族自治区重点研发计划(重大)重点项目（2019BFG02011）。
第一作者：张君。研究方向：植物生物学。E-mail：1340131221@qq.com
*通信作者：陈任，博士，教授。研究方向：植物分子生物学与基因工程。E-mail：chenren511@163.com
更新日期/Last Update: