[1]许静静,田云龙,魏博,等. 杉木根系接种AMF染色条件的筛选及观察[J].西北林学院学报,2023,38(4):166-172.[doi:10.3969/j.issn.1001-7461.2023.04.21]
　XU Jing-jing,TIAN Yun-long,WEI Bo,et al. Screening and Observation of AMF Staining Conditions for Chinese Fir Roots Inoculation[J].JOURNAL OF NORTHWEST FORESTRY UNIVERSITY,2023,38(4):166-172.[doi:10.3969/j.issn.1001-7461.2023.04.21]
点击复制

 杉木根系接种AMF染色条件的筛选及观察()
分享到：

《西北林学院学报》[ISSN:1001-7461/CN:61-1202/S]

卷:
第38卷
期数:
2023年第4期
页码:
166-172
栏目:
出版日期:
2023-07-31

文章信息/Info

Title:
 Screening and Observation of AMF Staining Conditions for Chinese Fir Roots Inoculation
文章编号:
1001-7461(2023)04-0166-07
作者:
 许静静1; 2; 田云龙1; 2; 魏博1; 2; 马祥庆1; 2; 吴鹏飞1; 2*
 （1.福建农林大学 林学院，福建 福州 350002；2.福建省杉木种质创新工程研究中心，福建 福州 350002）
Author(s):
 XU Jing-jing1; 2; TIAN Yun-long1; 2; WEI Bo1; 2; MA Xiang-qing1; 2; WU Peng-fei1; 2*
 (1.Forestry College,Fujian Agriculture and Forestry University,Fuzhou 350002,Fujian,China; 2.Fujian Engineering Research Center of Chinese Fir Germplasm Enhancement,Fuzhou 350002,Fujian,China)
关键词:
 ; 杉木; 根级; 丛枝菌根真菌; 染色方法; 侵染率
Keywords:
 ; Chinese fir;  root order;  arbuscular mycorrhizal fungus;  staining method;  infection rate
分类号:
S718.8
DOI:
10.3969/j.issn.1001-7461.2023.04.21
文献标志码:
A
摘要:
 丛枝菌根真菌（AMF）侵染情况观察是深入研究植物根系与土壤微生物共生机制的重要基础。以杉木同一无性系1年生幼苗为研究对象，在接种根内球囊霉（Glomus intraradices,Gi）3个月后，基于经典的台盼蓝染色法，利用正交试验设计，对影响杉木根系染色效果的KOH浓度、温度以及染色时间等主要因素进行优化研究，比较分析杉木不同根序的AMF侵染率差异性规律。结果表明，对于杉木幼苗1级根，筛选出20%的KOH浓度、水煮温度90 ℃和染色时间30 min这一组合的染色效果较好，显微制片中的根系皮层组织细胞、菌丝结构清晰；对于杉木2级根，采用20% KOH浓度、水煮温度96 ℃和染色时间10 min处理下的染色效果较好；相对木质化程度最高的3级根，采用20% KOH浓度、水煮温度100 ℃、染色时间30 min或10% KOH浓度、水温96 ℃、染色时间20 min这2种组合的染色效果较好。与杉木幼苗1级根和3级根相比，2级根的AMF侵染率明显较高（P<0.05）。
Abstract:
 The observation of arbuscular mycorrhizal fungi (AMF) infection is an important basis for the further study of the symbiotic mechanisms between plant root system and soil microorganisms.In this study,objected with annual seedlings cultivated from the same Chinese fir clone,using an orthogonal test design based on the classical Taipan Blue staining method,the main factors of the staining effect in Chinese fir root system tissues,including KOH concentration,boiling water temperature and staining time were optimized respectively,after the tested seedlings were inoculated with Glomus intraradices (Gi) for three months.Furthermore,the differences of AMF infection rate among different root orders were compared and analyzed.Main results showed that the combination conditions with 20% KOH concentration,water temperature of 90 ℃ and staining time of 30 min were better for the first order roots of Chinese fir seedlings,and both of the cellular and mycelial structures of the root cortex in the microfilm were clear.For the second order of Chinese fir roots,the staining effect was better under the treatment with 20% KOH concentration,water temperature of 96 ℃ and staining time of 10 min.Both of the two combination conditions of 20% KOH concentration,water temperature of 100 ℃ and staining time of 30 min or 10% KOH concentration,water temperature of 96 ℃ and staining time of 20 min were more effective in staining the tertiary roots with the highest relative lignification.Compared with the first or third order of Chinese fir roots,AMF infestation rate in the second order root was significantly higher (P<0.05).

相似文献/References:

[1]戴益源,丁永年.屏边县杉木林生产力评价[J].西北林学院学报,2001,16(04):16.
　Assessment on the Productivity of Chinese Fir in Pingbian County,Yunnan Province[J].JOURNAL OF NORTHWEST FORESTRY UNIVERSITY,2001,16(4):16.
[2]谢会成,姜志林,尹建道.杉木的光合特性及其对CO2倍增的响应[J].西北林学院学报,2002,17(02):1.
　Photosynthetic Characteristics of 13-year-old Chinese Fir Current Shoots and Its Responses to Elevated CO2[J].JOURNAL OF NORTHWEST FORESTRY UNIVERSITY,2002,17(4):1.
[3]符韵林,徐峰,韦广绥,等.南带产区杉木正常木与被压木的比较解剖研究[J].西北林学院学报,2005,20(03):160.
　FU Yun-lin,XU Feng,WEI Guang-sui,et al.Wood Anatomy Contrast of Chinese Fir(Cunninghamia lanceolata)for Normal Tree and Suppressed Tree in Southern Growing District[J].JOURNAL OF NORTHWEST FORESTRY UNIVERSITY,2005,20(4):160.
[4]涂育合.杉木不同经营密度的林下植被变化[J].西北林学院学报,2005,20(04):52.
　TU Yu-he.Dynamic Characteristics of Undergrowth Vegetations in Cunninghamia lanceolata with Different Management Density[J].JOURNAL OF NORTHWEST FORESTRY UNIVERSITY,2005,20(4):52.
[5]徐明,任海青,郭伟.我国杉木材性变异研究进展[J].西北林学院学报,2008,23(01):185.
　XU Ming,REN Hai-qing,GUO Wei.Research Progress of Variations for Wood Properties of Chinese Fir[J].JOURNAL OF NORTHWEST FORESTRY UNIVERSITY,2008,23(4):185.
[6]黄艳辉,赵荣军通讯作者赵荣军。,费本华.杉木木材微纤丝角变异规律的研究[J].西北林学院学报,2007,22(01):119.
　HUANG Yanhui,ZHAO Rongjun,FEI Benhua.Variation Patterns of Microfibril Angle for Chinese Fir Wood[J].JOURNAL OF NORTHWEST FORESTRY UNIVERSITY,2007,22(4):119.
[7]李佳,邵全琴,黄麟,等.我国马尾松、杉木、湿地松生长方程研究进展[J].西北林学院学报,2010,25(04):151.
[8]王迪海,赵忠,张彦. 黄土高原刺槐细根与土壤水分特征[J].西北林学院学报,2012,27(01):1.
　WANG Di-hai,ZHAO Zhong,ZHANG Yan. The Fine Root of Robinia pseudoacacia and Soil Moisture in the Loess Plateau[J].JOURNAL OF NORTHWEST FORESTRY UNIVERSITY,2012,27(4):1.
[9]耿生莲. 不同土壤水分下黑果枸杞生理特点分析[J].西北林学院学报,2012,27(01):6.
　GENG Sheng lian. Physio Characteristics of Lycium ruthenicum under Different Soil Water Conditions[J].JOURNAL OF NORTHWEST FORESTRY UNIVERSITY,2012,27(4):6.
[10]乌凤章. 辽南地区越橘根系分布特征[J].西北林学院学报,2012,27(01):11.
　WU Feng zhang. Characteristics of Root System of Blueberry in South of Liaoning Province[J].JOURNAL OF NORTHWEST FORESTRY UNIVERSITY,2012,27(4):11.
[11]李婷婷,郑小贤,宁杨翠,等. 出材率表简易编制方法研究——以浙江省开化林场人工林为例[J].西北林学院学报,2012,27(01):155.
　LI Ting-ting,ZHENG Xiao-xian,NING Yang-cui,et al. A Simple Method for Construction of Timberproduced Rate Table ——A Case of Kaihua Forest Farm[J].JOURNAL OF NORTHWEST FORESTRY UNIVERSITY,2012,27(4):155.
[12]袁晓红,李际平. 杉木人工林南北坡向树高-胸径生长曲线研究[J].西北林学院学报,2012,27(02):180.
　YUAN Xiao-hong,LI Ji-ping. Height, DBH Growth Models of Fir Artificial Forest on Northern and Southern Slopes[J].JOURNAL OF NORTHWEST FORESTRY UNIVERSITY,2012,27(4):180.
[13]刘丽颖,张绍轩,任佳佳,等. 30年生不同生长势杉木的胸径和树高的生长动态[J].西北林学院学报,2014,29(06):171.[doi:doi:10.3969/j.issn.1001-7461.2014.06.32]
　LIU Li-ying,ZHANG Shao-xuan,REN Jia-jia,et al. DBH and Tree Height Growth Dynamics of Cunninghamia lanceolata of Different Growth Degree[J].JOURNAL OF NORTHWEST FORESTRY UNIVERSITY,2014,29(4):171.[doi:doi:10.3969/j.issn.1001-7461.2014.06.32]
[14]刘素真,孙玉军*. 土壤养分与杉木生长的相关性研究[J].西北林学院学报,2015,30(05):15.[doi:doi:10.3969/j.issn.1001-7461.2015.05.03]
　LIU Su-zhen,SUN Yu-jun*. Correlation Between Soil Nutrients and the Growth of Chinese Fir[J].JOURNAL OF NORTHWEST FORESTRY UNIVERSITY,2015,30(4):15.[doi:doi:10.3969/j.issn.1001-7461.2015.05.03]
[15]姜鹏,庞立欣,邵思祺,等. 不同生长势杉木成熟林的生物量生长模型研究[J].西北林学院学报,2016,31(3):36.[doi:10.3969/j.issn.1001-7461.2016.03.06]
　JIANG Peng,PANG Li-xin,SHAO Si-qi,et al.Biomass Model of Cunninghamia lanceolata with Different Growth Potentials[J].JOURNAL OF NORTHWEST FORESTRY UNIVERSITY,2016,31(4):36.[doi:10.3969/j.issn.1001-7461.2016.03.06]
[16]郭祥泉. “正态分布标准差分类法”对杉木养分供求响应的研究[J].西北林学院学报,2016,31(5):132.[doi:10.3969/j.issn.1001-7461.2016.05.22]
　GUO Xiang-quan. Application of Standard Deviation Classification Method of Normal Distribution to the Response of Cunninghamia lanceolata for the Nutrient Supply[J].JOURNAL OF NORTHWEST FORESTRY UNIVERSITY,2016,31(4):132.[doi:10.3969/j.issn.1001-7461.2016.05.22]
[17]周垂帆,林静雯,李莹,等. 草甘膦对土壤磷形态及有效性的影响[J].西北林学院学报,2016,31(6):71.[doi:10.3969/j.issn.1001-7461.2016.06.13]
　ZHUO Chui-fan,LIN Jing-wen,LI Ying,et al. Effects of Glyphosate on Inorganic Phosphorus Transformation in Soil[J].JOURNAL OF NORTHWEST FORESTRY UNIVERSITY,2016,31(4):71.[doi:10.3969/j.issn.1001-7461.2016.06.13]
[18]洪宜聪. 杉木林套种闽粤栲林分特性及其涵养水源功能[J].西北林学院学报,2017,32(3):71.[doi:10.3969/j.issn.1001-7461.2017.03.14]
　HONG Yi-cong. Stand Characteristics and Water Conservation Function of Cunninghamia lanceolata Plantation Mixed with Castanopsis fissa[J].JOURNAL OF NORTHWEST FORESTRY UNIVERSITY,2017,32(4):71.[doi:10.3969/j.issn.1001-7461.2017.03.14]
[19]邱岭军,胡欢甜,林宝平,等. 不同林龄杉木养分重吸收率及其C∶N∶P化学计量特征[J].西北林学院学报,2017,32(4):22.[doi:10.3969/j.issn.1001-7461.2017.04.04]
　QIU Ling-jun,HU Huan-tian,LIN Bao-ping,et al. Nutrient Resorption Efficiency and C∶N∶P Stoichiometry of Cunninghamia lanceolata Plantations with Different Ages[J].JOURNAL OF NORTHWEST FORESTRY UNIVERSITY,2017,32(4):22.[doi:10.3969/j.issn.1001-7461.2017.04.04]
[20]饶丽莎,许珊珊*,黄田盛,等. 杉木Cu/Zn-SOD基因克隆、序列特征及组织特异性表达[J].西北林学院学报,2018,33(2):75.[doi:10.3969/j.issn.1001-7461.2018.02.12]
　RAO Li-sha,XU Shan-shan*,HUANG Tian-sheng,et al. Cloning,Sequence Characterization and Tissue Specific Expression of Cu/Zn-SOD Gene in Chinese Fir[J].JOURNAL OF NORTHWEST FORESTRY UNIVERSITY,2018,33(4):75.[doi:10.3969/j.issn.1001-7461.2018.02.12]

备注/Memo

备注/Memo:
 收稿日期：2022-05-20 修回日期：2022-07-04
基金项目：福建省自然科学基金重点项目（2020J02029）；福州市科学技术局科技计划项目（2021-P-035）。
第一作者：许静静。研究方向：水土保持。E-mail:xujj0221@foxmail.com
*通信作者：吴鹏飞，教授，博士，博士生导师。研究方向：森林培育和水土保持。E-mail:fjwupengfei@126.com
更新日期/Last Update: