[1]焦嘉卉,符晓,张硕,等. 不同林龄花椒根际土壤理化性质与微生物群落结构的分析[J].西北林学院学报,2023,38(4):156-165.[doi:10.3969/j.issn.1001-7461.2023.04.20]
　JIAO Jia-hui,FU Xiao,ZHANG Shuo,et al. Physiochemical Properties and Microorganism Community Structure of Zanthoxylum bungeanum Rhizosphere Soil at Different Ages[J].JOURNAL OF NORTHWEST FORESTRY UNIVERSITY,2023,38(4):156-165.[doi:10.3969/j.issn.1001-7461.2023.04.20]
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 不同林龄花椒根际土壤理化性质与微生物群落结构的分析()
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《西北林学院学报》[ISSN:1001-7461/CN:61-1202/S]

卷:
第38卷
期数:
2023年第4期
页码:
156-165
栏目:
出版日期:
2023-07-31

文章信息/Info

Title:
 Physiochemical Properties and Microorganism Community Structure of Zanthoxylum bungeanum Rhizosphere Soil at Different Ages
文章编号:
1001-7461(2023)04-0156-10
作者:
 焦嘉卉1; 符晓1; 张硕1; 刘伟2; 周姣姣1; 吴旭艳1; 林晓榕1; 田雨露3; 唐光辉1*; 李培琴1*
 （1.西北农林科技大学 林学院，西部森林生物灾害治理国家林业和草原局重点试验室，陕西 杨陵 712100；2.洛阳市林业生态建设发展中心，河南 洛阳 471000；3.眉县国有营头林场，陕西 宝鸡 722307）
Author(s):
 JIAO Jia-hui1; FU Xiao1; ZHANG Shuo1; LIU Wei2; ZHOU Jiao-jiao1; WU Xu-yan1; LIN Xiao-rong1; TIAN Yu-lu3; TANG Guang-hui1*; LI Pei-qin1*
 (1.Key Laboratory of National Forestry and Grassland Administration on Management of Western Forest Bio-Disaster,Forestry of College,Northwest A&F University,Yangling 712100,Shaanxi,China;2.Luoyang Forestry Ecological Construction and Development Center,Luoyang 471000,Henan,China;3.Yingtou State Owed Forest Farm of Meixian,Baoji 722307,Shaanxi,China)
关键词:
 ; 花椒; 林龄; 根际微生物; 土壤理化性质; 群落结构
Keywords:
Zanthoxylum bungeanum;  forest age;  rhizosphere microorganism;  soil physiochemical property;  community structure
分类号:
S763.81
DOI:
10.3969/j.issn.1001-7461.2023.04.20
文献标志码:
A
摘要:
 根际微生物是土壤生态系统的重要组成部分，明确植物根际土壤微生物的群落结构及影响因素对农林生态系统的可持续发展具有重要的意义。结合高通量测序技术及土壤理化性质测定，分析不同林龄花椒根际土壤理化性质的变化与微生物的群落结构差异。结果表明，随着林龄增加，花椒根际土壤呈现弱酸化，土壤养分整体表现出先增加而后降低的趋势，以8 a林龄根际土的养分最高；花椒根际土中含真菌OTU 1 229个，归属于12门、35纲、78目、149科、321属和466种；细菌OTU 1 298个，归属于22门、49纲、71目、163科和277属。Alpha多样性分析发现，林龄对花椒根际土壤真菌的物种丰富度和多样性影响显著，以8 a林龄的物种丰富度和多样性最高，15 a林龄的次之，3 a林龄的最低；而林龄对花椒根际土壤细菌的物种丰富度和多样性影响不显著。在3个不同林龄的花椒根际土壤中，优势真菌门均为子囊菌门(Ascomycota)和罗兹菌门(Rozellomycota)，优势属均为缘刺盘菌属(Cheilymenia)和镰孢菌属(Fusarium)；优势细菌门为变形菌门(Proteobacteria)和酸杆菌门(Acidobacteria)，优势属为鞘氨醇单胞菌属(Sphingomonas)、酸杆菌门Gp6和Gp4群组和芽单胞菌属(Gemmatimonas)；且发现不同林龄花椒根际土壤微生物的群落结构分离明显，说明林龄是微生物群落结构的主要影响因子。与花椒根际土壤微生物的Alpha多样性显著相关的土壤理化因子为pH、有机质、速效磷和速效钾，而影响花椒根际土壤微生物群落结构的重要土壤理化因子为有机质、总氮和pH。研究不仅阐明了林龄对花椒根际土壤理化性质与微生物群落结构的影响，还揭示了土壤理化性质与花椒根际土壤微生物群落结构之间的关系，不仅能为椒园土壤质量变化的动态监测提供依据，还能为花椒根际土壤微生物资源的功能开发奠定基础。
Abstract:
 Rhizosphere microorganism is an important part of soil ecosystem.It is meaningful to confirm the community structure of rhizosphere soil microorganisms and its influence factors for the sustainable development of agroforestry ecosystem.High-throughput sequencing technology and physiochemical peoperties detection of soil were combined in this study,aiming to analyze the variation of soil physiochemical properties and differences of rhizosphere microorganisms community structure as the change of Zanthoxylum bungeanum age.The results showed that the rhizosphere soil of Z.bungeanum showed weak acidification and the soil nutrients,as a whole,increased first and then decreased as the increase of planting years.The nutrients in the rhizosphere soil of the 8-year-old Z.bungeanum were the highest.Totally,1 229 fungal operational taxonomic units (OTUs) were identified in Z.bungenum rhizosphere soil,belonging to 12 phyla,35 classes,78 orders,149 families,321 genera and 466 species,while 1 298 bacterial OTUs were obtained,belonging to 22 phyla,49 classes,71 orders,163 families and 277 genera.By analyzing Alpha diversity,it was found that the ages of Z.bungenum showed significant effects on the species richness and diversity of rhizosphere fungi,of which the 8-year-old Z.bungenum exhibited the highest richness and diverisity,followed by the 15-year-old,and the 3-year-old displayed the lowest.However,the ages of Z.bungenum showed no significant effects on the bacterial richness and diversity.In the rhizosphere soil of all the three different ages of Z.bungenum,the dominant fungal phyla and genera were respectively Ascomycota and Rozellomycota,and Cheilymenia and Fusarium,while the dominant bacterial phyla and genera were respectively Proteobacteria and Acidobacteria,and Sphingomonas,Acidobacteria GP4,Acidobacteria GP6,and Gemmatimonas.Moreover,it was found that the community structures of rhizosphere microorganisms were obviously separated by the age of Z.bungenum,which indicated that the age was the dominant influence factor on microorganisms community structures.The physiochemical factors of soil,which showed the obvious relationship with the Alpha diversity of rhizosphere microorganisms of Z.bungeanum,were pH,organic matter,available phosphorus and available potassium.Whereas,the important influence factors on the microorganisms community structures were organic matter,total nitrogen and pH.This study not only demonstrates the effects of Z.bunbgeanum ages on the soil physiochemical properties and microorganism community structure,but also reveals the relationship between the soil physiochemical properties and variation of microorganism community structure,which can not only provide the basis for dynamic monitoring of the soil physiochemical properties in the Z.bunbgeanum plantation,but also lay the foundation for the function exploitation of rhizosphere microorganism of Z.bunbgeanum.

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备注/Memo

备注/Memo:
 收稿日期：2022-05-30 修回日期：2022-10-07
基金项目：国家级大学生创新创业训练项目（S202010712169）；陕西省重点研发计划（2020NY-037）；西北农林科技大学科技创新与成果转化项目（TGZX2021-23）。
第一作者：焦嘉卉。研究方向：森林病理学。E-mail：jjh665605@163.com
*通信作者：唐光辉，教授，博士生导师。研究方向：林业有害生物防治。E-mail：tanggh@nwsuaf.edu.cn
李培琴，副教授，硕士生导师。研究方向：森林病理学。E-mail：lipq@nwsuaf.edu.cn
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