[1]王冬梅,朱玮,张存莉,等.卷叶黄精总皂苷含量测定方法及提取工艺研究[J].西北林学院学报,2006,21(03):107.
　WANG Dongmei,ZHU Wei,ZHANG Cunli,et al.Content Determination and Extraction of Steroids Saponins from the Root of Polygonatum cirrhifolium[J].JOURNAL OF NORTHWEST FORESTRY UNIVERSITY,2006,21(03):107.
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卷叶黄精总皂苷含量测定方法及提取工艺研究()
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《西北林学院学报》[ISSN:1001-7461/CN:61-1202/S]

卷:
第21卷
期数:
2006年03期
页码:
107
栏目:
林产化学
出版日期:
2006-06-30

文章信息/Info

Title:
Content Determination and Extraction of Steroids Saponins from the Root of Polygonatum cirrhifolium
作者:
王冬梅1; 朱玮2; 张存莉1; 张天拯1
1. 西北农林科技大学 林学院，陕西 杨陵 712100; 2.西北农林科技大学生命科学院，陕西 杨陵 712100
Author(s):
WANG Dongmei1; ZHU Wei2; ZHANG Cunli1; ZHANG Tianzheng1
1.College of Forestry, Northwest A F University, Yangling, Shaanxi 712100, China;2.College of Life Sciences,Northwest A F University, Yangling, Shaanxi 712100, China
关键词:
卷叶黄精;  皂苷; 含量测定; 提取分离工艺
Keywords:
Polygonatum cirrhifolium;  steroids saponins; content determination;  extraction
分类号:
S759.82
文献标志码:
A
摘要:
对分布于秦岭的卷叶黄精（Ploygonatum cirrhifolium）总皂苷含量测定方法及提取工艺进行了研究，结果表明，①黄精总皂苷含量测定采用香草醛-高氯酸比色法，在波长545 nm有最大吸收峰，在1.257.5 μg·mL-1范围内呈良好的线性关系。卷叶黄精总皂苷测定重现性较好，变异系数\%(RSD)\%为4.05%，平均回收率为96.40%，但测定工作要在30 min内完成。②各因素对卷叶黄精总皂苷的提取率影响不同，影响最大的是温度，其次是料液比和时间。最佳提取工艺为，在温度60℃下，用80%乙醇提取2次，每次提取时间为1.5 h，料液比为1∶15。西北林学院学报21卷第3期王冬梅等卷叶黄精总皂苷含量测定方法及提取工艺研究
Abstract:
The content determnation and extraction of steroids saponins from the root of Ploygonatum cirrhifolium were studied. The results are as follows ①when the methods of vanillinperchloric acid was applied to dertermine the content of steroids saponins at 545 nm, the linear range was 0.1260.642,the result was accurate, RSD was 4.05%. The average reclaimratio was 99.01%. ②The principal influencing factors influencing the extraction were temperature, the ratio of material to solvent and time.The best technological parameters for extracting steroids saponins were temperature 60℃, solvent 80% ethanol , time 1.5 h ; times of extraction 2; rate of material to solvent 1∶15 .

参考文献/References:

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相似文献/References:

[1]王冬梅,朱 玮,陈改侠,等.卷叶黄精根提取物的抗菌活性初步研究[J].西北林学院学报,2006,21(01):126.
　WANG Dongmei,ZHU Wei,CHEN Gaixia,et al.The Preliminary Study on Antibacterial Activity of Extracts from Polygonatum cirrhifolium Root[J].JOURNAL OF NORTHWEST FORESTRY UNIVERSITY,2006,21(03):126.

备注/Memo

备注/Memo:
收稿日期 20050829修回日期 20050927基金项目 西北农林科技大学科研专项基金（04 ZM027）作者简介 王冬梅（1970），女，陕西西安人，讲师，在读博士，主要从事天然产物化学的教学科研工作。
更新日期/Last Update: 2010-05-04