[1]王小玲,樊军锋通讯作者樊军锋(9),男,等.奥地利黑松组培苗生根技术研究[J].西北林学院学报,2007,22(01):63-66.
 WANG Xiaoling,FAN Junfeng,YU Faxin,et al.Study on Inducing Roots from Pinus nigra var. austriaca Plantlets[J].JOURNAL OF NORTHWEST FORESTRY UNIVERSITY,2007,22(01):63-66.
点击复制

奥地利黑松组培苗生根技术研究()
分享到:

《西北林学院学报》[ISSN:1001-7461/CN:61-1202/S]

卷:
第22卷
期数:
2007年01期
页码:
63-66
栏目:
林木培育与育种
出版日期:
2007-01-30

文章信息/Info

Title:
Study on Inducing Roots from Pinus nigra var. austriaca Plantlets
作者:
王小玲1樊军锋2通讯作者樊军锋(1963)陕西扶风人副研究员主要从事树木遗传改良和品种选育工作。余发新1王娟娟2
1.江西省科学院 生物资源研究所,江西 南昌 330029;2.西北农林科技大学 林学院,陕西 杨陵 712100
Author(s):
WANG Xiaoling1FAN Junfeng2YU Faxin1WANG Juanjuan2
1.Biological Resources Institute, Jiangxi Academy of Sciences, Nanchang, Jiangxi 330029, China;2.College of Forestry, Northwest A&F University, Yangling, Shaanxi 712100, China
关键词:
奥地利黑松组培苗试管内生根试管外生根
Keywords:
Pinus nigra var. austriaca plantletsrooting intuberooting outtube
分类号:
S791.259.05
文献标志码:
A
摘要:
本试验采用了试管内生根和试管外生根两种方法(1)把长度>3 cm的奥地利黑松无根组培苗接种到含有不同激素和碳源的1/2DCR、WPM、1/2GD培养基中生长;(2)把长度>5 cm的奥地利黑松无根组培苗,蘸取不同浓度的IBA、IAA、NAA溶液,转移至营养土中生长。结果表明试管内生根优于试管外生根,试管内生根的最佳培养基为WPM,并添加NAA0.02 mg·L-1+IBA0.5 mg·L-1+20 g·L-1蔗糖,;其生根率为55%。
Abstract:
There were two methods in inducing roots rooting intube and rooting out-tube. In the same culture condition , ① Select bigger than 3 centimeters Pinus nigra var. austriaca plantlets vaccinatesed 1/2DCR、WPM、1/2GD mediums to induce roots, which includes different kinds of hormones and sucroses; ②Select bigger than 5 centimeters Pinus nigra var. plantlets dipped in different concentration IBA、IAA、NAA solution, then shifts them to the nutrition soil to grow. The result showed that rooting intube was better and the best medium was WPM, adding NAA 0.01 mg·L-1+IBA0.5 mg·L-1+sucrose 20 g·L-1,on which the rate of rooting was 55%.

参考文献/References:

[1]Ramage CM,Williams RR.Mineral nutrition and plant morphyogenesis[J].In Virto Cell Dev BiolPlant,2002,38116124.
[2]Uderst G,Moncousin C,O'Rourke J.Stimulation of root formation in difficulttoroot wood cuttings by dithiothreitol[J].Int J Plant Sei,1997,158(2)132135.
[3]王怀智.植物造林与组织培养通过组织培养繁殖木本植物[J],林业科学,1982,19(3)292301.
[4]黄健秋,卫志明.松属树种的组织培养和原生质体培养[J].植物学通报,1994,11(1)3442.
[5]周健,成浩,王丽鸳.激素处理对茶树组培苗温室内直接诱导生根的影响[J].茶叶科学,2005,25(4)265269.
[6]王爱民,刘文,傅中滇.不同碳源对红边朱蕉组培苗生长的影响[J].徐州师范大学学报, 2003,21(3)7678.
[7]沈惠娟.木本植物组织培养技术[M].北京中国农业出版社,1992.1645.
[8]JangJC;TainterFH.Micropropagation of shortleaf,Virginia and loblolly X .shortleaf pine hybrids via organogenesis.Plant Cell[J],Tissue and Organ .Culture,1991,25(1)6167.

相似文献/References:

[1]王小玲,樊军锋,贾小明.奥地利黑松离体胚培养诱导不定芽的研究[J].西北林学院学报,2005,20(04):72.
 WANG Xiao-ling,FAN Jun-feng,JIA Xiao-ming.A Study of Adventitious Bud Inducing from Mature Zygotic Embryos Derived from Pinus nigra var. austriaca[J].JOURNAL OF NORTHWEST FORESTRY UNIVERSITY,2005,20(01):72.
[2]李华,苏娟,王华,等.不同处理对酿酒葡萄品种‘爱格丽’组培苗移栽的影响[J].西北林学院学报,2011,26(01):68.
[3]周永学,龚月桦,樊军锋,等.4种松树抗寒性的比较[J].西北林学院学报,2011,26(05):69.
[4]樊军锋,杨培华,周永学,等.奥地利黑松引种育苗试验[J].西北林学院学报,2004,19(01):52.
 Introduction and Seedling Cultivation of Pinus nigra var.austriaca[J].JOURNAL OF NORTHWEST FORESTRY UNIVERSITY,2004,19(01):52.
[5]陈星星,秦性英,郭丽,等. 昼夜温差对白掌组培苗生长的影响[J].西北林学院学报,2017,32(2):165.[doi:10.3969/j.issn.1001-7461.2017.02.28]
 CHEN Xing-xing,QIN Xing-ying,GUO Li,et al. Effects of Day-night Temperature Difference on the Growth of Spathiphyllum Plantlets in vitro[J].JOURNAL OF NORTHWEST FORESTRY UNIVERSITY,2017,32(01):165.[doi:10.3969/j.issn.1001-7461.2017.02.28]
[6]夏思哲,李铁梅,李凤菊,等. 野生葡萄‘燕山-1’ב河岸-3’种间杂交F1代植株耐盐性鉴定[J].西北林学院学报,2017,32(1):150.[doi:10.3969/j.issn.1001-7461.2017.01.24]
 XIA Si-zhe,LI Tie-mei,LI Feng-ju,et al. Identification of Salt Tolerance of in vitro F1 Hybrid Progenies of Yanshan-1×Hean-3[J].JOURNAL OF NORTHWEST FORESTRY UNIVERSITY,2017,32(01):150.[doi:10.3969/j.issn.1001-7461.2017.01.24]
[7]覃林海,韦素婕,王芳,等. 不同培养基对红心杉组培苗增殖及其生理的影响[J].西北林学院学报,2017,32(3):122.[doi:10.3969/j.issn.1001-7461.2017.03.23]
 QIN Lin-hai,WEI Su-jie,WANG Fang,et al. Effects of Different Media on the Seedling Growth and Physiology in Cunninghamia lanceolata[J].JOURNAL OF NORTHWEST FORESTRY UNIVERSITY,2017,32(01):122.[doi:10.3969/j.issn.1001-7461.2017.03.23]

备注/Memo

备注/Memo:
收稿日期20060421修回日期20060529基金项目国家林业局“948”项目(20000403)作者简介王小玲(1979),女,陕西白水人,助理研究员,主要从事林木遗传育种和林木生物技术研究工作。
更新日期/Last Update: 2010-05-04