[1]王艳,杜鸿云,梁海永,等.过路黄的组织培养及叶片植株再生[J].西北林学院学报,2008,23(04):104.
　WANG Yan,DU Hong yun,LIANG Hai yong*,et al.Tissue Culture and Plant Regeneration from the Leaves of Lysimachia christinae[J].JOURNAL OF NORTHWEST FORESTRY UNIVERSITY,2008,23(04):104.
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过路黄的组织培养及叶片植株再生()
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《西北林学院学报》[ISSN:1001-7461/CN:61-1202/S]

卷:
第23卷
期数:
2008年04期
页码:
104
栏目:
林木遗传育种
出版日期:
2008-07-30

文章信息/Info

Title:
Tissue Culture and Plant Regeneration from the Leaves of Lysimachia christinae
作者:
王艳; 杜鸿云; 梁海永; 等
1.河北农业大学 园林与旅游学院 河北 保定 071001；2.河北农业大学，河北 保定 071001； 3.河北农业大学 林学院，河北 保定 071001
Author(s):
WANG Yan1;  DU Hongyun2;  LIANG Haiyong3*;  LIU Guilin1
1．College of Landscape Architecture and Tourism，Agricultural University of Hebei,Baoding, Hebei 071001,China; 2. Agricultural University of Hebei,Baoding, Hebei 071001, China;3.College of Forestry, Agricultural University of Hebei,Baoding, Hebei 071001,China
关键词:
过路黄;  组织培养;  叶片再生
Keywords:
Lysimachia christinae ;  tissue culture;  plant regeneration
分类号:
S688.403.54
文献标志码:
A
摘要:
用过路黄嫩茎作为外植体进行组织培养，在附加不同浓度激素的培养基上对其进行增殖、生根培养，诱导叶片愈伤组织的形成及叶片愈伤组织不定芽的形成，建立其叶片再生体系。结果表明，过路黄最适分化培养基是MS+6BA 0.5 mg/L+NAA 1.0 mg/L，最适生根培养基是不附加任何激素的1/2MS培养基， 诱导叶片愈伤组织最适培养基是MS+6BA 0.3 mg/L+NAA 0.1 mg/L，诱导叶片愈伤组织不定芽形成最适培养基是MS+6BA 0.3 mg/L+NAA 0.1 mg/L。
Abstract:
The infancy stem segments of Lysimachia christinae were taken as explants to be cultured in the media supplemented with different levels of hormone to investigate its proliferation, rooting, formation of leaf callus and adventitious bud from leaf callus, and to establish leaf regeneration system. The resulted showed that preferable propagation medium was MS+6BA 0.5 mg/L+NAA 1.0 mg/L, rooting medium was 1/2MS, medium for leaf callus induction was MS+6BA 0.3 mg/L+NAA 0.1 mg/L.

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备注/Memo

备注/Memo:
收稿日期：20071215修回日期：20080106 基金项目： 河北省交通厅重点攻关项目(Y050116)。作者简介：王艳，女，在读硕士，主要从事园林植物资源利用及评价方面研究。 *通讯作者：梁海永，男，高级实验师，主要从事林业生物技术及仪器分析方面的研究。
更新日期/Last Update: 2008-07-30