[1]吴静,李永华,何松林,等.牡丹根系RNA提取方法的比较研究[J].西北林学院学报,2010,25(05):60-63.
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牡丹根系RNA提取方法的比较研究()
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《西北林学院学报》[ISSN:1001-7461/CN:61-1202/S]

卷:
第25卷
期数:
2010年05期
页码:
60-63
栏目:
林木遗传育种与培育
出版日期:
2010-09-30

文章信息/Info

作者:
吴静;李永华;何松林;
河南农业大学林学院;
关键词:
牡丹根系RNA提取改良CTAB法
摘要:
【目的】探索牡丹根系RNA提取的最佳方法。【方法】以牡丹(Paeonia suffruticosa)'凤丹'5a生实生苗的根系为材料,首次比较研究了改良Trizol法、改良CTAB法、改良异硫氰酸胍法及Column Plant RNAout试剂盒法提取RNA的效果。【结果】改良Trizol法及改良异硫氰酸胍法均不能从牡丹根系中提取出高质量RNA。而改良CTAB法及试剂盒法提取RNA的电泳结果可见清晰完整的28S rRNA1、8S rRNA及5S rRNA条带,其中28S rRNA与18S rRNA的亮度比约为2∶1;浓度及纯度检测表明这2种方法提取的RNA纯度较高,且改良CTAB法提取的RNA浓度(269.50μg.g-1)是试剂盒法(82.14μg.g-1)的3倍。【结论】结合RNA的提取质量和成本,改良CTAB法更适宜于牡丹根系RNA的提取。

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备注/Memo

备注/Memo:
河南省高校创新人才培养工程(豫教高[2005]126号)
更新日期/Last Update: 2010-09-20