[1]苑子夜,苏印泉*,张强,等.DPPH·法评价杜仲叶提取物的抗氧化活性[J].西北林学院学报,2011,26(06):119-123.
　YUAN Zi ye,SU Yin quan,ZHANG Qiang,et al.Evaluation of Antioxidant Activity of Eucommia ulmoides Leaf Extracts by DPPH· Assay[J].JOURNAL OF NORTHWEST FORESTRY UNIVERSITY,2011,26(06):119-123.
点击复制

DPPH·法评价杜仲叶提取物的抗氧化活性
分享到：

《西北林学院学报》[ISSN:1001-7461/CN:61-1202/S]

卷:
第26卷
期数:
2011年06期
页码:
119-123
栏目:
林产化学
出版日期:
2011-11-30

文章信息/Info

Title:
Evaluation of Antioxidant Activity of Eucommia ulmoides Leaf Extracts by DPPH· Assay
作者:
苑子夜1; 苏印泉1*; 张强1; 朱铭强1; 中泽庆久2; 堤雅史2
1.西北农林科技大学 林学院， 陕西 杨陵 712100；2.大阪大学, 日本 大阪府 5650871
Author(s):
YUAN Ziye1; SU Yinquan1; ZHANG Qiang1; ZHU Mingqiang1;  NAKAZAWA Yoshihisa2; TSUTSUMI Masafumi2
1.College of Forestry, Northwest A&F University, Yangling, Shaanxi 712100, China; 2. Osaka University,Osaka Prefecture 5650871,Japan
关键词:
杜仲叶; 抗氧化活性; 提取工艺; DPPH·; 总多酚; 总黄酮
Keywords:
Eucommia ulmoides leaves;  antioxidant activity;  extracting technology;  DPPH·;  total phenols;  total flavonoids
分类号:
S789.4
文献标志码:
A
摘要:
采用超声波法提取杜仲叶抗氧化物质，用DPPH·法评价其抗氧化活性。采用不同极性溶剂对最佳提取条件下的提取物进行萃取，测定不同极性萃取物中的总多酚和总黄酮含量及DPPH·清除率。结果表明：超声波法的最佳提取工艺为：60%乙醇，料液比1∶15，提取温度60℃，提取时间30 min，此工艺条件下DPPH·清除率为91.01%。杜仲叶抗氧化活性物质主要存在于其弱极性的乙酸乙酯部分，该部分100 μg·mL-1质量浓度样液的DPPH·清除率达92.28%，强于相同质量浓度的BHT，与相同质量浓度的Vc无显著差异。相关分析表明，杜仲叶抗氧化活性的主要物质基础为总多酚和总黄酮。
Abstract:
The antioxidant components of Eucommia ulmoides leaves were extracted by ultrasonicassisted method and their antioxidant activities were investigated by scavenging DPPH·. The extracts obtained from optimum technological conditions were further extracted by different polar organic solvents and the contents of total phenolic compounds, total flavonoids and DPPH radical scavenging activity were determined. The results showed that the optimal parameters of the ultrasonicassisted extraction were as follows: ethanol concentration 60%, solid to liquid ratio 1∶15, temperature 60℃ and extracting time 30 min. Under these conditions, the clearance rate to DPPH· were 91.01%. The bioactive antioxidants of E. ulmoides leaves were mainly attributed to the ethyl acetate fraction. The percentage of scavenging DPPH radical of the sample of the ethyl acetate fraction with the concentration of 100 μg·mL-1 was 92.28%, stronger than those of BHT (p<0.05) and no significant differences compared with those of Vc. The result of correlation analysis showed that the strong antioxidant activity mainly resulted from the high contents of the total phenols and total flavonoids.

参考文献/References:

［1］于瑞涛, 梅丽娟, 陶燕铎, 等. 大孔树脂NKA9分离纯化杜仲叶中的绿原酸［J］. 分析试验室, 2007, 26(增):56.
[2]周华珠, 陈翠华, 孙云，等. 杜仲叶提取物对衰老小鼠抗氧化功能的影响［J］. 徐州医学院学报, 1998, 18(6):463464.
[3]尉芹, 马希汉. 杜仲叶和元宝枫叶提取物抗氧化性能的研究［J］. 食品科学, 1998, 19(8):35.
[4]ZHANG Q, SU Y Q, YANG F X, et al. Antioxidative activity of water extracts from leaf, male flower, raw cortex and fruit of Eucommia ulmoides Oliv ［J］. Forest Products Journal, 2007, 57(12):7478.
[5]董大鹏. 杜仲叶中抗氧化活性成分的分离和提取［D］. 贵阳: 贵州省发酵工程及生物制药实验室, 2006.
[6]YEN G C, HSIEH C L. Antioxidant actions of Duzhong (Eucommia ulmoides Oliv.) toward oxidative damage in biomolecules ［J］. Life Sciences, 2000, 66:13871400.
[7]YEN G C，HSIEH C L. Inhibitory effects of Duzhong (Eucommia ulmoides Oliv.) against lowdensity lipoprotein oxidative modification ［J］. Food Chem, 2002, 77:449456.
[8]SUN T, HO C T. Antioxidant activities of buckwheat extracts ［J］. Food Chemistry, 2005, 90(4):743749.
[9]邹青松, 李素霞, 陈山, 等. DPPH·法评价瑶山甜茶浸膏的抗氧化性能［J］. 食品科技, 2010, 35(1):209212. ZOU Q S, LI S X, CHEN S, et al. Evaluation of antioxidant activity of Rubus suavissimus, Lee extracts by DPPH· assay ［J］. Food Science and Technology, 2010, 35(1):209212.
[10]彭长连, 陈少薇, 林植芳, 等. 用清除有机自由基DPPH法评价植物抗氧化能力［J］. 生物化学与生物物理进展, 2010, 35(1):209212. PENG C L, CHEN S W, LIN Z F, et al. Detection of antioxidative capacity in plants by scavenging organic free radical DPPH ［J］. Prog．Biochem．Biophys, 2010, 35(1):209212.
[11]严敏, 李崎, 顾国贤. 一个评价啤酒老化程度的新指标——DPPH清除率［J］. 食品科技, 2006, 31(3):9193. YAN M, LI Q, GU G X. A new index to estimate the extent of beer staling——The DPPH· scavenging ratio ［J］. Food Science and Technology, 2006, 31(3):9193.
[12]JIANG S H, WANG C L, CHEN Z Q, et al. Antioxidant properties of the extract and subfractions from old leaves of Toona sinensis roem (melisceae) ［J］. Journal of Food Biochemistry, 2009, 33(3):425441.
[13]翟梅枝, 郭景丽, 王磊, 等. 栾树花黄酮类化合物的提取工艺研究［J］. 西北林学院学报, 2010, 25(2):136139. ZHAI M Z, GUO J L, WANG L, et al. Extraction of flavonoids from Koelreuteria paniculata flowers ［J］. Journal of Northwest Forestry University, 2010, 25(2):136139.
[14]乔冬冬, 张云, 尤新军, 等. 星点设计效应面法优化梓树果实总黄酮的超声提取工艺［J］. 西北林学院学报, 2010, 25(3):170174. QIAO D D, ZHANG Y, YOU X J, et al. Optimization of the ultrasonic wave aided extraction of flavoniods from the fruits of Catalpa ovata by central composite designesponse surface methodology ［J］. Journal of Northwest Forestry University, 2010, 25(3):170174.
[15]傅燕玲, 吴晓琴. 杨梅枝多酚的抗氧化活性研究［J］. 中国粮油学报, 2009, 24(11):7882. FU Y L, WU X Q. Antioxidant effect of polyphenol extracted from Myrica rubra branches ［J］. Journal of the Chinese Cereals and Oils Association, 2009, 24(11):7882.
[16]阮征, 胡筱波, 赖富饶, 等. 油菜花粉中黄酮类物质提取工艺的优化研究［J］. 食品科学, 2007, 28(7):133137. RUAN Z, HU X B, LAI F R, et al. Extraction technology of flavones in cole pollen ［J］. Food Science, 2007, 28(7):133137.
[17]董娟娥, 马柏林, 刘丽, 等. 超声提取杜仲叶中有效成分工艺研究［J］. 西北林学院学报, 2003, 16(3):6668. DONG J E, MA B L, LIU L, et al. An ultrasonic extraction method of Eucommia ulmoides leaves ［J］. Journal of Northwest Forestry University, 2003, 16(3):6668.
[18]刘宁, 仇农学, 田玉霞. 超声辅助提取紫苏叶黄酮及其清除自由基作用的研究［J］. 西北林学院学报, 2008, 23 (1):158161. LIU N, CHOU N X, TIAN Y X. Ultrasonicaided extraction of flavonoids from Perilla frutescens(L.) Brltt leaves and evaluation for freeadical scavenging［J］. Journal of Northwest Forestry University, 2008, 23 (1):158161.
[19]RICEEVANS C A, MILLER N J, PAGANGA G. Structureantioxidant activity relationships of flavonoids and phenolic acids ［J］. Free Radical Biology & Medicine, 1996, 20:933956.
[20]江慎华, 王书源, 马海乐, 等. 丁香活性物质提取工艺优化与抗氧化活性研究［J］. 农业机械学报, 2010, 41(1):133138. JIANG S H, WANG S Y, MA H L, et al. Extracting technology and antioxidant activity of bioactive components from clove［J］. Transactions of the Chinese Society for Agricultural Machinery, 2010, 41(1):133138.

相似文献/References:

[1]吴洪新,阿拉木斯,李薇,等.二色胡枝子黄酮抗氧化活性的研究二色胡枝子黄酮抗氧化活性的研究[J].西北林学院学报,2009,24(02):108.
　WU Hong xin,Alamusi,LI Wei,et al.Antioxidative Activity of Flavonoids from Lespedeza bicolor[J].JOURNAL OF NORTHWEST FORESTRY UNIVERSITY,2009,24(06):108.
[2]董娟娥,马柏林,贾二红.杜仲叶中桃叶珊瑚甙测定方法的研究[J].西北林学院学报,2001,16(01):53.
　DONG Juan-e,MA Bai-lin,JIA Er-hong.Study on Measuring Method of Aucubin in Eucommia ulmoids Leaf[J].JOURNAL OF NORTHWEST FORESTRY UNIVERSITY,2001,16(06):53.
[3]马梅,马希汉,张檀,等.杜仲叶采收时间与采叶量的研究[J].西北林学院学报,1999,14(03):0.
　Ma Mei,Ma Xihan,Zhang Tan,et al.Time and Quantity of Leaf Collection of of Eucommia ulmoides Oliv[J].JOURNAL OF NORTHWEST FORESTRY UNIVERSITY,1999,14(06):0.
[4]龚晓武,李炳奇,刘丹丹,等.红景天黄酮提取及其抗氧化活性研究[J].西北林学院学报,2011,26(03):136.
[5]庞明,李西柳,陆刚,等.丹参须根乙醇提取物抗氧化及抑真菌活性研究[J].西北林学院学报,2011,26(04):196.
[6]吴媛琳,赵听,张凯煜,等. 枇杷不同部位主要有效成分含量及抗氧化活性比较[J].西北林学院学报,2015,30(01):196.[doi:doi:10.3969/j.issn.1001-7461.2015.01.32]
　WU Yuan-lin,ZHAO Ting,ZHANG Kai-yu,et al. Main Active Ingredient Contents and the Antioxidant Activity of Different Parts of Eriobotrya japonica[J].JOURNAL OF NORTHWEST FORESTRY UNIVERSITY,2015,30(06):196.[doi:doi:10.3969/j.issn.1001-7461.2015.01.32]
[7]胡瑞瑞,苏印泉*,朱铭强. 不同精致工艺杜仲枝木醋液抑菌及抗氧化活性比较[J].西北林学院学报,2014,29(02):139.[doi:doi:10.3969/j.issn.1001-7461.2014.02.26]
　HU Rui-rui,SU Yin-quan*,ZHU Ming-qiang. Research on Antimicrobial and Antioxidant Activities of Pyroligneous Acids of Eucommia ulmoides Branch Dealing with Different Refined Methods[J].JOURNAL OF NORTHWEST FORESTRY UNIVERSITY,2014,29(06):139.[doi:doi:10.3969/j.issn.1001-7461.2014.02.26]
[8]何德飞,苏印泉*. 无机絮凝剂纯化杜仲叶水提液条件优化研究[J].西北林学院学报,2014,29(02):202.[doi:doi:10.3969/j.issn.1001-7461.2014.02.36]
　HE De-fei,SU Yin-quan*. The Optimum Condition in the Purification of Water Extract of Eucommia ulmoides Leaves with Inorganic Flocculant[J].JOURNAL OF NORTHWEST FORESTRY UNIVERSITY,2014,29(06):202.[doi:doi:10.3969/j.issn.1001-7461.2014.02.36]
[9]苏锐,樊金拴*,郑涛,等. 长柄扁桃仁多酚提取工艺及抗氧化活性研究[J].西北林学院学报,2015,30(02):206.[doi:doi:10.3969/j.issn.1001-7461.2015.02.36]
　SU Rui,FAN Jin-shuan*,ZHENG Tao,et al. Extraction of Polyphenols from Amygdalus pedunculata Seed and the Antioxidant Capacity[J].JOURNAL OF NORTHWEST FORESTRY UNIVERSITY,2015,30(06):206.[doi:doi:10.3969/j.issn.1001-7461.2015.02.36]
[10]韦苑妮,赵忠*,朱海兰. 不同产地山杏油脂含量及抗氧化活性差异研究[J].西北林学院学报,2015,30(04):109.[doi:doi:10.3969/j.issn.1001-7461.2015.04.18]
　WEI Yuan-ni,ZHAO Zhong*,ZHU Hai-lan. Research on Differences of Apricot Kernels Oil Content and Antioxidant Activity Between Different Habitats[J].JOURNAL OF NORTHWEST FORESTRY UNIVERSITY,2015,30(06):109.[doi:doi:10.3969/j.issn.1001-7461.2015.04.18]

备注/Memo

备注/Memo:
收稿日期：20101021修回日期：20110301 基金项目：中日合作课题(K332020908)；陕西省日元贷款项目(K332020023) 作者简介：苑子夜，女，在读硕士研究生，主要研究方向为植物资源化学与生物活性物质。 *通讯作者：苏印泉，男，教授，主要从事植物资源利用研究。Email：syq009@126.com。
更新日期/Last Update: 2011-12-31