[1]吴洪新,陈晨. 尖叶胡枝子总黄酮提取与纯化研究[J].西北林学院学报,2014,29(02):196-201.[doi:doi:10.3969/j.issn.1001-7461.2014.02.35]
　WU Hong-xin,CHNE Chen. Extraction and isolation of Flavonoids From Lespedeza hedysaroides[J].JOURNAL OF NORTHWEST FORESTRY UNIVERSITY,2014,29(02):196-201.[doi:doi:10.3969/j.issn.1001-7461.2014.02.35]
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 尖叶胡枝子总黄酮提取与纯化研究()
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《西北林学院学报》[ISSN:1001-7461/CN:61-1202/S]

卷:
第29卷
期数:
2014年02期
页码:
196-201
栏目:
出版日期:
2014-03-30

文章信息/Info

Title:
 Extraction and isolation of Flavonoids From Lespedeza hedysaroides
文章编号:
1001-7461（2014）-02-0196-06
作者:
 吴洪新1; 陈晨2
 （1.中国农业科学院草原研究所, 内蒙古呼和浩特010010；2.内蒙古农业大学, 内蒙古呼和浩特010010）
Author(s):
 WU Hong-xin1;  CHNE Chen2
 (1. Grassland Research Institute of CAAS , Huhhot , Inner Mongolia 010010, China; 2. Inner Mongolia Agricultural University, Huhhot, Inner Mongolia 010010, China)
关键词:
 ; 尖叶胡枝子; 黄酮; 提取; 分离与纯化
Keywords:
Lespedeza hedysaroides;  flavonoid;  extraction;  isolation and purification
分类号:
S718.43
DOI:
doi:10.3969/j.issn.1001-7461.2014.02.35
文献标志码:
A
摘要:
 以总黄酮提取率为考查指标, 采用单因素试验和正交试验方法对尖叶胡枝子中黄酮类化合物的提取工艺进行优化, 最佳提取工艺为：70%的乙醇，提取温度为70℃，固液比为1∶20，提取时间为2 h。同时，将提取的粗黄酮进一步的分离纯化，最终得到8个黄酮类的化合物，分别为荭草素、异荭草素、牡荆苷、异牡荆苷、异杨梅树皮苷、槲皮素-3-O-β-D-葡萄糖苷、木犀草素-7-O-葡萄糖苷及6-xylopyranosylluteolin。尖叶胡枝子富含有较好药理活性的碳苷黄酮，作为一种植物资源，具有广阔的开发利用前景。
Abstract:
 Optimum conditions of extracting flavonoids from lespedeza hedysaroides were investigated by orthogonal test. The extract was further purified. The optimal conditions of the extraction were solvent:70% ethanol, temperature: 70℃, ratio of material to solvent:1∶20, and extraction time: 2 h. Eight flavonoids were isolated by chromatographic method, and their structures were identified as : orientin, isoorientin, vitexin，isovitexin, isomyricitrin, meletin-3-O-β-d-glucoside, luteolin-7-O-glucoside, and 6-xylopyranosylluteolin.in which in the flavonoids were mostly C-glycosides.

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备注/Memo

备注/Memo:
 收稿日期：2013-06-27 修回日期：2013-08-15
基金项目：中央级公益性科研院所基本科研业务费专项基金(中国农业科学院草原研究所)。
作者简介：吴洪新，男，助理研究员，研究方向：牧草资源开发与利用。E-mail：wuhongxin168@163.com
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