[1]魏鹏,刘彤*,张元杭. ‘库尔勒香梨’微繁生根培养基大量元素优化方法的比较[J].西北林学院学报,2014,29(04):127-131.[doi:doi:10.3969/j.issn.1001-7461.2014.04.23]
　WEI Peng,LIU Tong*,ZHANG Yuan-hang. Comparison Study of the Methods for Optimizing Macroelement Content of Rooting Medium for Micropropagation of Pyrus sinkiangensis cv. Korla Fragrant Plantlet[J].JOURNAL OF NORTHWEST FORESTRY UNIVERSITY,2014,29(04):127-131.[doi:doi:10.3969/j.issn.1001-7461.2014.04.23]
点击复制

 ‘库尔勒香梨’微繁生根培养基大量元素优化方法的比较()
分享到：

《西北林学院学报》[ISSN:1001-7461/CN:61-1202/S]

卷:
第29卷
期数:
2014年04期
页码:
127-131
栏目:
出版日期:
2014-07-30

文章信息/Info

Title:
 Comparison Study of the Methods for Optimizing Macroelement Content of Rooting Medium for Micropropagation of Pyrus sinkiangensis cv. Korla Fragrant Plantlet
文章编号:
1001-7461(2014)04-0127-05
作者:
 魏鹏1; 刘彤2*; 张元杭3
 （1. 宁夏职业技术学院 植物组培快繁中心，宁夏 银川 750002；2. 新疆兵团绿洲生态农业重点实验室，新疆 石河子 832003；3. 石河子大学 生命科学学院，新疆 石河子 832003）
Author(s):
 WEI Peng1;  LIU Tong2*;  ZHANG Yuan-hang3
 (1.Center of Plant Tissue Culture and Rapid Propagation, Ningxia Polytechnic College, Yinchuan , Ningxia 750002, China; 2.Key Laboratory of Oasis Ecological Agriculture of Xinjiang Bintuan, Shihezi, Xinjiang 832003，China; 3.College of Life Scicence, Shihezi University, Shihezi, Xinjiang 832003, China)
关键词:
 ; 库尔勒香梨; 营养成分分析; 组织培养; 生根培养基; 大量元素
Keywords:
Pyrus sinkiangensis cv. Korla Fragrant;  nutrient composition analysis;  plant tissue culture;  rooting medium;  macroelement
分类号:
Q945.3
DOI:
doi:10.3969/j.issn.1001-7461.2014.04.23
文献标志码:
A
摘要:
 以‘库尔勒香梨’（Pyrus sinkiangensis cv. Korla Fragrant）为研究材料，分析了‘库尔勒香梨’试管苗完整植株、田间植株新梢顶部未展开叶片与嫩茎、田间植株新梢叶片3种材料N、P、K、Ca、Mg 5种元素含量，采用Morard提出的调配方法折算试管苗植株5种元素含量折算得到M系列培养基，同时用Goncalves提出的方法对田间植株新梢茎叶、田间植株新梢叶片元素含量得到G系列培养基，对比分析了3种材料、2种调配方法所得8种培养基离子成分含量与试管苗生根效果。结果表明：M、G系列培养基NH+4、总NO-3、PO3-4含量差异较大；以新梢茎叶为基础的G1处理，生根率为92.7%，显著高于M系列配方，试管苗茎叶生长质量也具优势。G系列配方中G1生根率显著高于以新梢叶片为材料的G2处理。以‘库尔勒香梨’田间植株新梢茎叶为材料，按照Goncalves提出的方法，分析N、P、K、Ca、Mg 5种元素含量，调配微繁生根培养基大量元素含量，效果良好。
Abstract:
 Taking Pyrus sinkiangensis cv. Korla Fragrant as research object, the contents of N, P, K, Ca, and Mg that contained in 3 different materials were measured. The materials included the whole test-tube plantlet, shoot tips of the seedling in the field (including tender stems and unfolding leaves), and leaves of new tips in the field. Based on the methods put forward by Morard and Goncalves, M-series and G-series medium formulations were obtained for seedling propagation. Significant differences were found in the contents of [NH4+], total [NO3-], and [PO43-] between G and M series media. When the shoots collected from the field were cultured in medium G1, the rooting rate was 92.7%, significantly higher than those cultured in M series media, and the seedlings cultured by G1 also had advantages in the quality. Among G-series media, the rooting rate of the shoots cultured in G1 was higher than that in G2. It was concluded that best results could be achieved after following the method proposed by Goncalves to measure the contents of N, P, K, Ca, and Mg to optimize the ratios of the 5 elements in rooting media of P. sinkiangensis cv. Korla Fragrant.

参考文献/References:

 [1]曹孜义,刘国民. 实用植物组织培养技术教程[M].兰州:甘肃技术出版社,1996:102.
[2]张利彩,郭春会,孙占育,等. 普通扁桃试管苗生根培养研究[J].西北农业学报, 2008,17(2):188-192.
ZHANG L C, GUO CH H,SUN ZH Y,et al. Study on culture of in vitro rooting of almond [J]. Acta Agriculturae Boreali-occidentalis Sinica, 2008,17(2):188-192.(in Chinese)
[3]MORARD P, HENRY M. Optimization of the mineral composition of in vitro culture media[J]. Journal of Plant Nutrition,1998,21 (8):1565-1576.
[4]BABOULENE L, SILVESTRE J, MORARD P,et al. Effect of Ca deficiency on growth and leaf acid soluble proteins of Tomato[J]. Journal of Plant Nutrition, 2007,30(4):497-501.
[5]MONTEIRO A C B, HIGASHI E N, GONCALVES A N, et al. A novel approach for the definition of the inorganic medium components for micropropagation of yellow passionfruit (Passiflora edulis sims.flavicarpa deg.)[J]. In Vitro Cellular and Developmental Biology - Plant,2000,36:527-531.
[6]HAN B, BEN M, ANNEMIEK T. Development of new tissue culture media, suing the relation between mineral composition of plant and medium[J]. Acta Horticulture,2001,560:373-376.
[7]NAS M N, READ P E. A hypothesis for the development of a defined tissue culture medium of higher plants and micropropagation of hazelnuts[J]. Scientia Horticulture,2004,101:189-200.
[8]GONCALVES S, CORREIA P J, MARTINS M A, et al. A new medium formulation for in vitro rooting of carob tree based on leaf macronutrients concentrations[J]. Biologia Plantarum,2005,49(2):277-280.
[9]OSORIO M L, OSORIO J, GONCALVES S, et al. Carob trees (Ceratonia siliqua L.) regenerated in vitro can acclimatize successfully to match the field performance of seed-derived plants[J]. Trees,2012,26(6):1837-1846.
[10]刘彤,赵新俊,任丽彤,等. 新疆香梨试管苗最佳生根培养基的研究[J]. 果树学报, 2004, 21 (2):124-127.
LIU T, ZHAO X J, REN L T,et al. Research of optimum rooting medium for micropropagated pear plantlets[J]. Journal of Fruit Science, 2004, 21 (2):124-127. (in Chinese)
[11]何天明,刘泽军,覃伟铭,等. 土壤因子对库尔勒香梨缺铁失绿症发生的影响[J]. 西北农业学报,2013,22(1):97-103.
He T M,LIU Z J,QIN W M,et al. Effects of soil factors on iron-deficit chlorosis of kuerle fragrant pear(Pyrus bretschneideri Rehd) [J]. Acta Agriculturae Boreali-occidentalis Sinic,2013,22(1):97-103. (in Chinese)
[12]张元杭. 库尔勒香梨在试管内的适应特性研究[D]. 石河子：石河子大学,2008. 
[13]魏鹏. 植物离体繁殖的表型可塑性机理及培养基调控技术研究[D]. 石河子：石河子大学,2007.
[14]刘小勇,董铁,王发林,等. 甘肃省元帅系苹果叶营养元素含量标准值研究[J].植物营养与肥料学,2013,19(1):246-251.
LIU X Y,DONG T,WANG F L,et al. Studies on the standard ranges of leaf nutritional element contents of Delicious apple in Gansu province [J]. Plant Nutrition and Fertilizer Science,2013,19(1):246-251.(in Chinese)
[15]李海霞,李正华,郭树平,等. 不同氮磷水平对红松幼苗碳氮积累与分配的影响[J]. 西北林学院学报,2013,18( 5):24-29.
LI H X,LI ZH H,GUO S P,et al. Effects of different nitrogen and phosphorus supply levels on C,N accumulation and partitioning in Pinus koraiesis seedlings[J]. Journal of Northwest Forestry University,2013,18(5):24-29.(in Chinese)
[16]潘瑞炽. 植物生理学[M].6版. 北京:高等教育出版社,2008:29.
[17]崔秀梅,王玖瑞,刘孟军,等. 枣未成熟胚高效再生体系研究[J]. 西北林学院学报,2012,27(1):81-84.
CUI X M,WANG J R,LIU M J,et al. An efficient system for plant regeneration from immature embryo of Chinese jujub[J]. Journal of Northwest Forestry University,2012,27(1):81-84.(in Chinese)
[18]王慧梅, 王文杰, 董凤丽, 等. 影响喜树组织培养苗离体生根的因素[J]. 植物学通报, 2004, 21 (6): 673-681.
WANG H M,WANG W J, DONG F L,et al. Factors affecting in vitro rooting of Camptotheca acuminata [J]. Chinese Bulletin of Botany, 2004, 21 (6): 673-681.(in Chinese) 

相似文献/References:

[1]李亚藏,梁彦兰,王庆成. 铅对山梨和山荆子光合作用和叶绿素荧光特性的影响[J].西北林学院学报,2012,27(05):21.
　LI Ya-cang,LIANG Yan-lan,WANG Qing-cheng. Influence of Pb on Photosynthesis and Chlorophyll Fluorescence Characteristics in Pyrus ussuriensis and Malus baccata [J].JOURNAL OF NORTHWEST FORESTRY UNIVERSITY,2012,27(04):21.
[2]张小咏,李佳,杨艳昭,等. 基于SWAT模型的长江源区径流模拟[J].西北林学院学报,2012,27(05):38.
　ZHANG Xiao-yong,LI Jia,YANG Yan-zhao,et al. Runoff Simulation of the Catchment of the Headwaters of the Yangtze River Based on SWAT Model[J].JOURNAL OF NORTHWEST FORESTRY UNIVERSITY,2012,27(04):38.
[3]杨红旗,陈广辉,王金林. PVAc改性酚醛树脂制备铝木复合材料研究[J].西北林学院学报,2013,28(01):170.[doi:10.3969/j.issn.1001-7461.2013.01.34]
　YANG Hong-qi,CHEN Guang-hui,WANG Jin-lin. PF Adhesive Modified by PVAc for Manufacturing Technology of Decorative Fiberboard with Aluminum Foils[J].JOURNAL OF NORTHWEST FORESTRY UNIVERSITY,2013,28(04):170.[doi:10.3969/j.issn.1001-7461.2013.01.34]
[4]江涛,李秀荣,谢延军,等. 微波预处理对巨尾桉木材渗透性的影响[J].西北林学院学报,2013,28(01):174.[doi:10.3969/j.issn.1001-7461.2013.01.35]
　JIANG Tao,LI Xiu-rong,XIE Yan-jun,et al. Effects of Microwave Pretreatment on the Permeability of Eucalyptus grandis×Eucalyptus urophylla[J].JOURNAL OF NORTHWEST FORESTRY UNIVERSITY,2013,28(04):174.[doi:10.3969/j.issn.1001-7461.2013.01.35]
[5]牛晓霆,王逢瑚,曹新民. 明清家具匠师原木下料的工艺原则及措施[J].西北林学院学报,2013,28(01):178.[doi:10.3969/j.issn.1001-7461.2013.01.36]
　NIU Xiao-ting,WANG Feng-hu,CAO Xin-min. Techniques and Principles of Log Cuttings of Furniture Makers in Ming and Qing Dynasties[J].JOURNAL OF NORTHWEST FORESTRY UNIVERSITY,2013,28(04):178.[doi:10.3969/j.issn.1001-7461.2013.01.36]
[6]吕金阳,覃卓凯,舒辉,等. 银杉木材构造美学价值[J].西北林学院学报,2013,28(01):183.[doi:10.3969/j.issn.1001-7461.2013.01.37]
　LV Jin-yang,QIN Zhuo-kai,SHU Hui,et al. Esthetical Values in Wood Structure of Cathaya argyrophylla[J].JOURNAL OF NORTHWEST FORESTRY UNIVERSITY,2013,28(04):183.[doi:10.3969/j.issn.1001-7461.2013.01.37]
[7]杨艳红,张海敏,孙景荣. 基于传统文化视角的现代家具设计探析[J].西北林学院学报,2013,28(01):188.[doi:10.3969/j.issn.1001-7461.2013.01.38]
　YANG Yan-hong,ZHANG Hai-min,SUN Jing-rong. Study of Modern Furniture Design Based on Traditional Culture[J].JOURNAL OF NORTHWEST FORESTRY UNIVERSITY,2013,28(04):188.[doi:10.3969/j.issn.1001-7461.2013.01.38]
[8]黄圣游,叶喜. 新中式家具“中式”传统的界定[J].西北林学院学报,2013,28(01):192.[doi:10.3969/j.issn.1001-7461.2013.01.39]
　HUANG Sheng-You,YE Xi. Definition of Traditional Element in New Chinese Style Furniture[J].JOURNAL OF NORTHWEST FORESTRY UNIVERSITY,2013,28(04):192.[doi:10.3969/j.issn.1001-7461.2013.01.39]
[9]韩维生,赵明磊,王宏斌. 基于阈限理论的设计尺度体系[J].西北林学院学报,2013,28(01):197.[doi:10.3969/j.issn.1001-7461.2013.01.40]
　HAN Wei-sheng,ZHAO Ming-lei,WANG Hong-bin. Design Scale System Based on Threshold Theory[J].JOURNAL OF NORTHWEST FORESTRY UNIVERSITY,2013,28(04):197.[doi:10.3969/j.issn.1001-7461.2013.01.40]
[10]宋杰,侯建军,申黎明. 按摩椅按摩头包覆层对按摩舒适性的影响[J].西北林学院学报,2013,28(01):202.[doi:10.3969/j.issn.1001-7461.2013.01.41]
　SONG Jie,HOU Jian-jun,SHEN Li-ming. Influence of Massager Coat Layer of Massage Chairs on the Degree of Comfort[J].JOURNAL OF NORTHWEST FORESTRY UNIVERSITY,2013,28(04):202.[doi:10.3969/j.issn.1001-7461.2013.01.41]
[11]位杰,蒋媛*,马建江,等. 拉枝角度对库尔勒香梨枝叶生长及果实品质的影响[J].西北林学院学报,2018,33(3):111.[doi:10.3969/j.issn.1001-7461.2018.03.17]
　WEI Jie,JIANG Yuan*,MA Jian-jiang,et al. Effects of Branch Bending Angle on the Branch and Leaf Growth and Fruit Quality of Korla Fragrant Pear[J].JOURNAL OF NORTHWEST FORESTRY UNIVERSITY,2018,33(04):111.[doi:10.3969/j.issn.1001-7461.2018.03.17]

备注/Memo

备注/Memo:
 收稿日期：2013-10-30修回日期：2014-02-24
基金项目：国家自然科学基金(30360017)。
作者简介：魏鹏，男，硕士，讲师，研究方向：植物种苗快繁与植物生理生态。E-mail:weipeng135@126.com
*通信作者：刘彤，男，博士，教授，研究方向：植物组织培养与植物生态学。E-mail: betula@126.com
更新日期/Last Update: