[1]唐豆豆,李厚华*,张延龙,等. ‘凤丹’牡丹组织培养研究[J].西北林学院学报,2016,31(2):160-166.[doi:10.3969/j.issn.1001-7461.2016.02.28]
　TANG Dou-dou,LI Hou-hua*,ZHANG Yan-long,et al. Studies on Tissue Culture of Paeonia ostii ‘Feng Dan’[J].JOURNAL OF NORTHWEST FORESTRY UNIVERSITY,2016,31(2):160-166.[doi:10.3969/j.issn.1001-7461.2016.02.28]
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 ‘凤丹’牡丹组织培养研究()
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《西北林学院学报》[ISSN:1001-7461/CN:61-1202/S]

卷:
第31卷
期数:
2016年第2期
页码:
160-166
栏目:
出版日期:
2016-03-31

文章信息/Info

Title:
 Studies on Tissue Culture of Paeonia ostii ‘Feng Dan’
文章编号:
1001-7461(2016)02-0160-07
作者:
 唐豆豆1; 李厚华1*; 张延龙1; 马凯恒2; 包努恩·都特1; 李果1
 （1.西北农林科技大学 风景园林艺术学院，陕西 杨陵 712100；2.西北农林科技大学 林学院，陕西 杨陵 712100)
Author(s):
 TANG Dou-dou1; LI Hou-hua1*; ZHANG Yan-long1; MA Kai-heng2; BAO Nuendute1; LI Guo1
 (1.College of Landscape Architecture and Arts,Northwest A&F University,Yangling,Shaanxi 712100,China；2.College of Forestry,Northwest A&F University,Yangling,Shaanxi 712100,China)
关键词:
 ; ‘凤丹’牡丹; 组织培养; 扩繁; 生根
Keywords:
Paeonia ostii ‘Feng Dan’;  tissue culture;  proliferation;  rooting
分类号:
S685.11
DOI:
10.3969/j.issn.1001-7461.2016.02.28
文献标志码:
A
摘要:
 以‘凤丹’牡丹芽为材料，对其外植体消毒、褐化防治、组培苗扩繁和生根技术进行研究。结果表明：用75% 酒精消毒30 s，0.1% 升汞消毒8 min消毒效果最佳，外植体的污染率和成活率分别为32.31%和64.32%；其最适初代诱导和继代增殖培养基为MS+6-BA 1.0 mg·L-1+NAA 0.2 mg·L-1和MS+6-BA 1.0 mg·L-1+NAA 0.2 mg·L-1+PIC (毒莠定，Picloram) 1.0 mg·L-1，增殖系数为4.84；生根培养基为1/2MS+CaCl2 220 mg·L-1+IBA 3.0 mg·L-1+NAA 0.5 mg·L-1,生根率为74.30%；低温结合3 mg·L-1硝酸银培养可使褐化率降至29.15%。
Abstract:
 Buds of Paeonia ostii ‘Feng Dan’ were used as explants to study its culture techniques,such as disinfection,anti-browning,proliferation and rooting.Best disinfection result could be achieved by 75% ethanol for 30 s and 0.1% HgCl2 for 8 min.Under this treatment,the contamination rate reduced to 32.31% and the survival rate reached 64.32%.The most appropriate medium for explants inducing was the medium of MS+6-BA 1.0 mg·L-1+NAA 0.2 mg·L-1,and the most suitable medium for propagation was MS+6-BA 1.0 mg·L-1+NAA 0.2 mg·L-1+PIC (Picloram) 1.0 mg·L-1.The best rooting medium was 1/2MS+CaCl2(220 mg·L-1)+IBA 3.0 mg·L-1+NAA 0.5 mg·L-1,on which the rooting rate reached 74.30%.Browning rate could be controlled to 29.15% under the cultivation combined chilling with 3 mg·L-1 AgNO3.

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备注/Memo

备注/Memo:
 收稿日期：2015-04-22修回日期：2015-05-18
基金项目：林业公益性行业科研重大专项（201404701）;高等学校博士学科点专项科研基金（20120204120006）。
作者简介：唐豆豆，女，在读硕士，研究方向：园林植物分子生物学。E-mail：1747662070@qq.com
*通信作者：李厚华，男，副教授，研究方向：植物分子生物学。E-mail：lihouhua73@163.com
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