[1]程建国,纪昌海,罗燕*,等. 四川九龙山自然保护区林麝栖息地典型植被土壤微生物群落特征[J].西北林学院学报,2017,32(2):205-211.[doi:10.3969/j.issn.1001-7461.2017.02.35]
　CHENG Jian-guo,JI Chang-hai,LUO Yan*,et al. Microbial Community Characteristics of Typical Vegetation Soil at Forest Musk Deer Habitats in Sichuan Jiulong Mountain Nature Reserve[J].JOURNAL OF NORTHWEST FORESTRY UNIVERSITY,2017,32(2):205-211.[doi:10.3969/j.issn.1001-7461.2017.02.35]
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 四川九龙山自然保护区林麝栖息地典型植被土壤微生物群落特征()
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《西北林学院学报》[ISSN:1001-7461/CN:61-1202/S]

卷:
第32卷
期数:
2017年第2期
页码:
205-211
栏目:
出版日期:
2017-03-31

文章信息/Info

Title:
 Microbial Community Characteristics of Typical Vegetation Soil at Forest Musk Deer Habitats in Sichuan Jiulong Mountain Nature Reserve
文章编号:
1001-7461(2017)02-0205-07
作者:
 程建国1; 纪昌海2; 罗燕3*; 周鑫3
 (1.四川养麝研究所，四川 都江堰 611830；2.四川九龙山自然保护区，四川 苍溪 628400；3.四川农业大学 都江堰校区微生物学实验室，四川 都江堰 611830)
Author(s):
 CHENG Jian-guo1; JI Chang-hai2; LUO Yan3*; ZHOU Xin3
 (1.Sichuan Institute of Musk Deer Breeding,Dujiangyan,Sichuan 611830,China; 2.Sichuan Jiulong Mountain Nature Reserve，Cangxi,Sichuan 628400,China; 3.Lab of Microbiology,Dujiangyan Campus,Sichuan Agricultural University,Dujiangyan,Sichuan 611830,China)
关键词:
 ; 九龙山自然保护区; 林麝栖息地; 土壤化学性质; 土壤微生物
Keywords:
 ; Jiulong Mountain Nature Reserve;  forest musk deer habitat;  soil physicochemical property;  soil microorganism
分类号:
S714.3
DOI:
10.3969/j.issn.1001-7461.2017.02.35
文献标志码:
A
摘要:
 为了解四川九龙山自然保护区林麝栖息地典型植被（灌木林、针阔混交林和阔叶混交林）下土壤微生物群落特征，探讨土壤微生物类群对地表植被组成的影响。采用平板稀释法进行活菌计数与菌株分离，16S rRNA PCR 鉴定分离菌，常规方法测定土壤理化学性质。结果表明，栖息地各植被类型下共由11种细菌菌属组成；以芽孢菌占主要部分，韦氏芽胞杆菌（Bacillus weihenstephanensis）、巨大芽胞杆菌（Bacillus megaterium）和苏云金芽胞杆菌（Bacillus thuringiensis）为优势菌群。各植被类型土壤均以细菌为主，放线菌次之，真菌最少；土壤微生物总数排序为灌木林>针阔混交林>阔叶混交林；各植被类型下不同土层中微生物的分布趋势是0~20 cm>20~40 cm>40~60 cm。相关性分析结果表明，pH值与放线菌呈显著正相关性（R=0.676，P<0.05），总微生物与细菌总数呈极显著正相关性（R=1，P<0.01），总微生物与放线菌呈显著正相关性（R=1，P<0.01）。
Abstract:
 The objectives of this study were to explore the characteristics of soil microbial communities in the soils of typical vegetations (shrub forest,mixed coniferous and broad-leaved mixed forest) at forest musk deer habitat in Sichuan Jiulong Mountain Nature Reserve,and to examine the effects of soil microbials on the composition of ground vegetations.The number of soil colonies was determined by plate colony dilution method.Isolated soil bacteria and actinomycetes were identified by 16S rRNA PCR method.Soil physicochemical properties were determined by the conventional methods.Eleven species of microorganisms were identified.They were mainly consisted of Bacillus weihenstephanensis,B.megaterium and B.thuringiensis.Among the microorganisms isolated from the soil of three vegetation types,the number of bacteria (over 70%) represented absolute advantage,actinomycetes were followed,and no fungi were separated.Distribution trend in three soil types of microorganisms in the soil profile was 0-20>20-40>40-60 cm.The correlation analysis indicated that pH value was significantly and positively correlated to actinomycetes (R=0.676,P<0.05).Total microorganisms were significantly and positively correlated to total bacteria (R=1,P<0.01).The correlationship between total microorganismsl and actinomycetes was significant and positive (R=1,P<0.05).

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备注/Memo

备注/Memo:
 收稿日期：2016-09-12修回日期：2016-10-28
基金项目：四川省林业厅省级自然保护区重点物种科学研究项目；四川农业大学双支项目。
作者简介：程建国，男，副研究员，研究方向：林麝保护和利用。E-mail：447933018 @qq.com
*通信作者：罗燕，女，教授，博士，研究方向：野生动物保护及病原微生物与分子生物学。E-mail：lycjg@163.com
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