[1]臧国忠,陈尚武,张文,等.文冠果子叶同步胚的高效诱导及植株再生[J].西北林学院学报,2008,23(05):91.
　ZANG Guo zhong,CHEN Shang wu,ZHANG Wen,et al.Synchronical Somatic Embryogenesis and Plant Regeneration of Xanthoceras sorbifolia[J].JOURNAL OF NORTHWEST FORESTRY UNIVERSITY,2008,23(05):91.
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文冠果子叶同步胚的高效诱导及植株再生()
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《西北林学院学报》[ISSN:1001-7461/CN:61-1202/S]

卷:
第23卷
期数:
2008年05期
页码:
91
栏目:
出版日期:
2008-09-30

文章信息/Info

Title:
Synchronical Somatic Embryogenesis and Plant Regeneration of Xanthoceras sorbifolia
作者:
臧国忠1 ; 陈尚武2 ; 张文1; 马会勤1*
1. 中国农业大学 农学与生物技术学院，北京 100094; 2. 中国农业大学 食品科学与营养工程学院，北京 100083
Author(s):
ZANG Guozhong1 ; CHEN Shangwu2;  ZHANG Wen1;  MA Huiqin1*
1. College of Agriculture and Biotechnology, China Agricaltural Univerity, Beijing 100094,China; 2. College of Food Science and Nutritional Engineering, China Agricaltural Univerity,Beijing 100083,China
关键词:
文冠果; 同步胚; 液体培养; 植株再生
Keywords:
Xanthoceras sorbifolia ;  synchronical somatic embryo;  suspension tissue culture;  plant regeneration
分类号:
S723.132
文献标志码:
A
摘要:
以文冠果成熟种子的子叶为外植体，优化培养基配方，诱导收集胚性愈伤组织，建立了高效的同步胚液体培养扩增体系，同步胚转移到固体培养基上，实现了植株的高效再生，为文冠果转基因平台的建立打下了基础。结果表明，文冠果子叶外植体胚性愈伤组织诱导优化培养基为附加2,4D 2.0 mg·L-1、NAA 1.0 mg·L-1、6BA 1.0 mg·L-1和3%的蔗糖的MS固体培养基，可产生大量胚性愈伤组织；胚性细胞系经继代和初步扩增后，在附加2,4D 0.5 mg·L-1、NAA 0.5 mg·L-1、6BA 0.5 mg·L-1和2%的蔗糖的B5液体培养基中增殖产生大量的同步球形胚；球形胚转入NAA 0.25 mg·L-1、6BA 0.5 mg·L-1的B5固体培养基上，经心形胚、鱼雷形胚和子叶形胚，再生成植株。
Abstract:
The cotyledons of Xanthoceras sorbifolia were selected as explants to induce somatic callus, with an optimized protocol, somatic callus were selected and subcultured to establish a high frequency proliferation system of synchronical somatic embryo in liquid medium. When the liquid cultured embryos were transferred on solid germination medium, plantlets were regenerated, which lays a platform for the future gene transformation of X. sorbifolia. The optimized medium as follows. Induction of somatic callus: MS medium supplemented with 2.0 mg·L-12,4D, 1.0 mg·L-1NAA, 1.0 mg·L-16BA, 3% sucrose and 0.66% agar; Synchronical globular somatic embryo liquid culture medium: B5 with 0.5 mg·L-1 2,4D, 0.5 mg·L-1 NAA, 0.5 mg·L-1 6BA and 2% sucrose; Somatic embryos development and germination medium: solid B5 with 0.25 mg·L-1 NAA and 0.5 mg·L-1 6BA. Heartshaped, torpedoshaped, and cotyledonshaped embryos were observed in germination process.

参考文献/References:

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备注/Memo

备注/Memo:
收稿日期：20080116修回日期：20080311 作者简介：臧国忠，男，硕士，研究方向：果树育种。 *通讯作者：马会勤。Emil:hqma@cau.edu.cn
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