[1]李守勇,续九如,孙浩元.不同产地冬枣的RAPD分析[J].西北林学院学报,2006,21(05):89.
　LI Shouyong,XU Jiuru,SUN Haoyuan.RAPD Marker Analysis of Dongzao Jujube in Different Sites[J].JOURNAL OF NORTHWEST FORESTRY UNIVERSITY,2006,21(05):89.
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不同产地冬枣的RAPD分析()
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《西北林学院学报》[ISSN:1001-7461/CN:61-1202/S]

卷:
第21卷
期数:
2006年05期
页码:
89
栏目:
林木育种与培育
出版日期:
2006-10-30

文章信息/Info

Title:
RAPD Marker Analysis of Dongzao Jujube in Different Sites
作者:
李守勇1; 续九如2; 孙浩元3
1.北京市科委农村发展中心, 北京 100101；2.北京林业大学，北京 100083；3.北京市林果研究所，北京 100093
Author(s):
LI Shouyong1;  XU Jiuru2;  SUN Haoyuan3
1.Rural Development Center of Beijing Science and Technology Committee，Beijing 100101, China;2.College of Biological and Technology, Beijing Forestry University，Beijing 100083, China;3.Institute of Forestry and Pomology of Beijing, Beijing 100093, China
关键词:
冬枣; 分子标记; 遗传距离; 聚类分析
Keywords:
Zizyphus jujube cv. Dongzao;  molecular marker;  genetic distance;  cluster analysis
分类号:
S665.1
文献标志码:
A
摘要:
冬枣是中国优良的鲜食枣品种，但生产中常将晚熟枣品种与冬枣相混淆，严重影响了冬枣产业的发展。利用筛选的17个随机引物对6个不同产地冬枣材料进行了RAPD分析，共扩增出144条谱带，有95条为多态性带，比例为65.09%。利用UPGMA进行聚类分析，结果表明，在遗传距离为0.095时，供试材料可分为2类，即成武冬枣与其他产地冬枣并非一类，不同产地间冬枣也存在一定的遗传变异。西北林学院学报21卷第5期李守勇等不同产地冬枣的RAPD分析
Abstract:
Dongzao jujube is a kind of superior fresheating dates in China. But it has been always confused with other lateripe date cultivars in production and this influences the development of date industry. The genetic diversity among 6 Dongzao jujube materials from different areas was analyzed by RAPD. 144 bands were detected with 17 RAPD primers, and 95 bands (65.09%) were polymorphic. The UPGMA dendrogram of 6 cultivars were divided into 2 groups based on cluster analysis when the genetic distance was {0.095}. The results showed that Chengwu Dongzao did not belong to the Dongzao group and there was heredity variation among the different sites of Dongzao jujube.

参考文献/References:

［1］续九如, 李守勇, 张昌盛. 冬枣形态特征及其与其他晚熟枣品种的区别［Ｊ］. 河北林果研究, 2003,(1) 3842.
［2］刘孟军. RAPD技术在枣和酸枣种质鉴定上的应用研究［Ａ］. 中国科协第二届青年学术年会园艺学论文集［Ｃ］． 北京 北京农业大学出版社, 1995.
［3］宋 婉. 中国枣优良品种DNA指纹图谱的研究［Ｄ］. 北京林业大学(博士论文), 1999.
［4］沈向, 郑学勤, 任小林，等. 核果类基因组DNA提取和RAPD条件优选［Ｊ］． 山东农业大学学报, 1999, 12(1) 17.
［5］彭建营, 束怀瑞, 孙仲序，等. 枣RAPD技术体系建立与几个品种亲缘关系的研究［Ｊ］. 农业生物技术学报, 2000, 8(2) 155159.
［6］赵锦, 刘孟军. 枣树RAPD分析条件的优化研究［Ｊ］. 果树学报, 2001, 18(6) 329332.
［7］陈桂信, 潘东明, 吕柳新，等. 果树核DNA提取、目的基因分离与克隆技术研究进展［Ｊ］. 福建农林大学学报(自然科学版),2002, 22(3) 4449@

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备注/Memo

备注/Memo:
收稿日期20051216修回日期20060116基金项目国家林业局“冬枣良种区域化试验研究”项目（2002-14）作者简介李守勇，（1975-），男，山东济南人，博士，从事农业科技项目管理工作。
更新日期/Last Update: 2010-05-04