[1]马勤,代侃韧,董丽芬,等.香椿的试管繁殖技术[J].西北林学院学报,2006,21(05):106.
　MAQin,DAI Kanren,DONG LifenWEI Tianru.Propagation of Toona sinensis by Tissue Culture Technology[J].JOURNAL OF NORTHWEST FORESTRY UNIVERSITY,2006,21(05):106.
点击复制

香椿的试管繁殖技术()
分享到：

《西北林学院学报》[ISSN:1001-7461/CN:61-1202/S]

卷:
第21卷
期数:
2006年05期
页码:
106
栏目:
林木育种与培育
出版日期:
2006-10-30

文章信息/Info

Title:
Propagation of Toona sinensis by Tissue Culture Technology
作者:
马勤1; 代侃韧2; 董丽芬1; 魏天儒3
1.西北农林科技大学 林学院, 陕西 杨陵 712100；2.西安市长安区农牧局， 陕西 西安 710061;3.杨凌职业技术学院 林学系， 陕西 杨凌712100
Author(s):
MAQin1; DAI Kanren2; DONG Lifen1WEI Tianru3
1.College of Forestry,Northwest A & F University, Yangling, Shaanxi 712100,China;2.Agricultural and Animal Husbandry Bureau of Chang'an District,Xi'an, Shaanxi 710061 China;3.Forestry Department,Yangling Vocatinal Technical College,Yangling,Shaanxi 7121
关键词:
香椿; 芽增殖; 试管苗
Keywords:
Toona sinensis;  bud multiplication;  culture seedling
分类号:
S723.133
文献标志码:
A
摘要:
选取当年生半木质化、具腋芽的香椿茎段作为外植体，诱导腋芽萌发，进行芽苗增殖和生根培养。通过试验，筛选出了各阶段适宜的培养基组成及试管苗移栽条件。结果表明，香椿萌芽诱导中，MS基本培养基效果优于1/2MS；芽增殖阶段，适宜的培养基是MS+6BA0.2 mg/L＋GA3 2.0, 有效增殖倍数为3.38；在1/2MS+1.0 mg/L IBA中，试管苗生根率最高,可达69%；适宜的移栽时间是37月初，移栽基质以蛭石或和沙为好。西北林学院学报21卷第5期马勤等香椿的试管繁殖技术
Abstract:
The new semiligneous stem nodes of Toona sinensis with axillary buds was taken as explant cultured in vitro to induce axillary bud sprouting and bud multiplications.Medium supplemented with different plant hormones and concentations which suit to different stage of the culture were chosen.Results showed that MS culture medium was better than 1/2MS culture medium in period of induceing germination; The suitable culture medium for subculture and shoot multiplication was MS+6BA0.2 mg/L＋GA32.0,the effective propagation rate was 3.38. The most suitable culture medium for rooting was 1/2MS+1.0 mg/L IBA,the rooting rate was 69% and grown well; The suitable transplant time was among Mar. to July annually, vermiculite and sand should be chosen as transplant medium materials.

参考文献/References:

［1］吴丽君.香椿组培快繁效率的影响因子［J］.中南林学院学报,2005，25（2）2529.
［2］陈存及.阔叶树种栽培［M］.北京中国林业出版社，2000.
［3］吴国兴.香椿刺龙牙保护地栽培［M］.北京金盾出版社，2001.
［4］陈静芬.香椿栽培新技术［M］.北京金盾出版社，1997.
［5］韩德元.植物生长调节剂原理与应用［M］ .北京科学技术出版社，1997.
［6］李瑜明，那宁馨，陈绍宽，等.两种绣线菊的组培快繁技术［J］.中国林副特产，2000,（4）15.
［7］张小红，陈彦生，康 冰，等.激素对香椿腋芽增殖生长的效应［J］.西北植物学报，2001，21（4）756760.
［8］董丽芬，张小英，张宗勤，等.树莓的组织培养技术［J］.西北林学院学报，2003，18（2）4243.(上接第68页)现较大的波动性，秋冬季土壤水分缓慢回升，春季土壤水分强烈消耗，夏季受降水影响呈现出一定波动变化。果园土壤水分储量受降水、地形等因子的影响呈现一定变化规律。不同坡向果园土壤水分储量表现为阴坡果园>峁顶果园>半阴半阳坡果园>阳坡果园。坡面果园0200 cm的水分储量在果树生长季49月份均低于适宜含水量，为保证果园稳产、高产应进行果园补灌。

相似文献/References:

[1]崔宏安,陈铁山ˇ,范龙霞,等.陕西省种源香椿天然类型叶的结构与抗逆性研究[J].西北林学院学报,2008,23(03):39.
　CUI Hongan,CHEN Tieshan,FAN Longxia,et al.Natural Types of Leaf Structure and Resistance of Toona sinensis Sha anxi Provenance[J].JOURNAL OF NORTHWEST FORESTRY UNIVERSITY,2008,23(05):39.
[2]骆嘉言,林金国,李大岔,等.香椿人工林和天然林木材材性的比较研究[J].西北林学院学报,2003,18(02):77.
　Comparative Study on Properties of Toona sinensis Wood from Plantation and Natural Forest[J].JOURNAL OF NORTHWEST FORESTRY UNIVERSITY,2003,18(05):77.
[3]崔宏安,陈铁山ˇ,范龙霞,等.陕西省种源香椿天然类型叶的结构与抗逆性研究[J].西北林学院学报,2008,23(02):39.
　CUI Hongan,CHEN Tieshan,FAN Longxia,et al.Natural Types of Leaf Structure and Resistance of Toona sinensis Sha anxi Provenance[J].JOURNAL OF NORTHWEST FORESTRY UNIVERSITY,2008,23(05):39.
[4]李平平,苗秀琴,武军杨,等.壳聚糖在香椿种子萌发及幼苗生长上的生理效应[J].西北林学院学报,2011,26(06):44.
　LI Ping ping,MIAO Xiu qin,WU Jun yang,et al.Physiological Effects of Chitosan on Seeds Germintion and Seedling Growth of Toona sinensis[J].JOURNAL OF NORTHWEST FORESTRY UNIVERSITY,2011,26(05):44.
[5]马正强,张贝贝,张京芳*,等. 热烫温度与pH值对香椿维生素C稳定性的影响[J].西北林学院学报,2015,30(03):201.[doi:doi:10.3969/j.issn.1001-7461.2015.03.35]
　MA Zheng-qiang,ZHANG Bei-bei,ZHANG Jing-fang*,et al. Effects of Blanching Temperature and pH on the Stability of Vitamin C of Toona sinensis[J].JOURNAL OF NORTHWEST FORESTRY UNIVERSITY,2015,30(05):201.[doi:doi:10.3969/j.issn.1001-7461.2015.03.35]
[6]胡继文,杨桂娟,刘振华,等. 不同坡位香椿人工林生长和林分结构差异[J].西北林学院学报,2021,36(5):82.[doi:10.3969/j.issn.1001-7461.2021.05.12]
　HU Ji-wen,YANG Gui-juan,LIU Zhen-hua,et al. Growth and Stand Structure Differences of Toona sinensis Plantation from Different Slope Positions[J].JOURNAL OF NORTHWEST FORESTRY UNIVERSITY,2021,36(05):82.[doi:10.3969/j.issn.1001-7461.2021.05.12]

备注/Memo

备注/Memo:
收稿日期20060406修回日期20060515基金项目陕西省科学院科研计划项目。作者简介马 勤（1969），女，河南项城人，助理研究员，主要从事期刊编辑和林业方面的工作。
更新日期/Last Update: 2010-05-04