[1]韦原莲,黄宝灵,吕成群,等.马占相思根瘤菌生物学特性的研究[J].西北林学院学报,2006,21(05):120.
　WEI Yuanlian,HUANG Baoling,LU Chengqun,et al.Biological Characteristics of Rhizobia in Acacia mangium[J].JOURNAL OF NORTHWEST FORESTRY UNIVERSITY,2006,21(05):120.
点击复制

马占相思根瘤菌生物学特性的研究()
分享到：

《西北林学院学报》[ISSN:1001-7461/CN:61-1202/S]

卷:
第21卷
期数:
2006年05期
页码:
120
栏目:
森林资源保护
出版日期:
2006-10-30

文章信息/Info

Title:
Biological Characteristics of Rhizobia in Acacia mangium
作者:
韦原莲; 黄宝灵; 吕成群; 张 慧; 樊利勤; 李清香
广西大学 林学院， 广西 南宁530005
Author(s):
WEI Yuanlian;  HUANG Baoling;  LU Chengqun;  ZHANG Hui;  FAN Liqin;  LI Qingxiang
College of Forestry Guangxi University, Nanning,Guangxi 530005,China
关键词:
马占相思; 根瘤菌; 生理生化特性; 抗逆性; 固N酶活性
Keywords:
Acacia mangium;  Rhizobia;  physiological and biochemical characteristics;  resistance;  nitrogenase activities
分类号:
S718.81
文献标志码:
A
摘要:
为了探讨马占相思根瘤菌的生物学特性，从广西高峰林场马占相思林分采集分离到9株根瘤菌，并对其生理生化特性、抗逆性和固N酶活性进行研究。结果表明，大部分菌株能较好利用供试糖源，所有供试菌株在3－酮基乳糖反应、牛肉膏蛋白胨、明胶水解、淀粉水解试验中呈阴性；在B．T．B反应、无机N源利用、柠檬酸盐利用呈阳性，硝酸盐还原试验大多呈阳性；石蕊牛奶试验除MZ02、MZ04产酸外，其余菌株产碱。所有供试菌株在温度30℃时生长最好，耐盐、耐酸碱范围较宽，MZ04在含盐浓度为2.0%环境还能生长良好，MZ04、MZ10在pH9.0环境仍能生长较好；所有菌株对250 μg /mL 庆大霉素（Sm3）均无抗性，不同菌株对其余供试抗菌素表现出不同的抗性，MZ02抗性最差，对所供试的抗生素均无抗性；MZ04、MZ08、MZ09抗性相对较强。所有供试菌株都具有固N酶活性，其中MZ02、MZ06、MZ08的固N酶活性最高。根据广西马占相思栽培区的生态条件，筛选出MZ02和MZ06为优良菌株。西北林学院学报21卷第5期韦原莲等马占相思根瘤菌生物学特性的研究
Abstract:
To study the biological characteristics of Rhizobia in Acacia mangium, 9 rhizobia strains were isolated from A. mangium in Gaofeng forest farm of Guangxi, and the physiological and biochemical characteristics, resistances and nitrogenase activities were detected, respectively. The results showed that sugar sources could be well used by most strains. In the experiments treated with 3ketolactose, beefprotein, gelatin hydrolysis and starch hydrolysis, all of testied strains were negative, In B.T.B reaction, inorganic nitrogen sources utilization and citrate utilization, the results were positive. Nitrate reduction experiment presented positive largely. In litmus milk experiment, all of strains produced alkai expect for MZ02 and MZ04 produced acid reactions. At 30℃, all of strains grew best, and had a wide range in salt, acid and basic resistance. MZ04 was capable of good growth at salt density of 2.0%, MZ04 and MZ10 could still grew better at pH 9.0. All of the strains were not resistant to 250 μg /mL gentamicin(Sm3), and to other antibiotics, different resistances were presented by different strains, MZ02 had worst resistance to all antibiotics, while MZ04, MZ08,MZ09 had strong resistance. All strains had nitrogenase activity, of which MZ02,MZ06 and MZ08 had higher nitrogenase activity. MZ02 and MZ06 were selected as fine strains according to the ecological conditions of cultural area of A. mangium in Guangxi.

参考文献/References:

［1］李庆逵.我国土壤科学发展与展望［Ｊ］.土壤学报.1989,26(3)207216.
［2］王新荣,康丽华.马占相思优势外生菌根与根瘤菌双接种效应初报［Ｊ］.林业科学究.1998,(11)542546.
［3］J.M.芬森特.根瘤菌实用研究手册［Ｍ］.上海上海人民出版社，1974.
［4］上海植物生理学会.植物生理学实验手册［Ｍ］．上海上海科学技术出版社，1985.
［5］中国科学院微生物研究所.一般细菌常用鉴定方法［Ｍ］.北京科学出版社，1978.
［6］东秀珠，蔡妙英．常见细菌系统鉴定手册［Ｍ］．北京科学出版社，2001.
［7］中国科学院微生物研究所.一般细菌常用鉴定方法［Ｍ］.北京科学出版社，1978.
［8］何正国，周俊初，李阜棣，等.16株快生型大豆根瘤菌生物学与分子生物学特性的比较研究［Ｊ］.农业基础与高科技研究进展，1996，1(1)1827.
［9］上海植物生理学会.植物生理学实验手册［Ｍ］．上海上海科学技术出版社，1985.
［10］黄健屏、朱桃英.林业微生物学［Ｍ］.湖南湖南科学技术出版社，1990.
［11］林德球.旋扭山绿豆快生型根瘤菌高度抗碱及共生固氮［Ｊ］.微生物学报，1989，29（5）354359.
［12］蓝 谷，蔡龙详，黄维南，等.四棱豆根瘤菌的分离及特性［J］.亚热带植物通讯1990，（2），1721.
［13］Trinick M J.Relationship amongst the fast growing rhizobia Leucaena leucocepha app［Ｊ］.J.Appl.Bacteriol,1980,493953.
［14］黄维南，蓝 谷，黄绳全，等.异叶银合欢根瘤菌的分离和特性［Ｊ］.亚热带植物通讯，1997，26(2)1217.
［15］康金花，关桂兰，沈艳芳.苜蓿根瘤菌耐盐碱性试验［Ｊ］.干旱区研究，1996，13（3）7477.
［16］杨江科，周 琴，周俊初. pH对土壤中土著快、慢生大豆根瘤菌结瘤的影响［Ｊ］．应用生态学报，2001，12（4）639640.
［17］王 静，马玉珍，史清亮，等．山西根瘤菌资源多样性与特异性研究［Ｊ］．应用与环境生物学报，1999，5（1）7984.西北林学院学报2006, 21(5)125128

相似文献/References:

[1]文晓萍,黄宝灵,吕成群,等.巨尾桉接种根瘤菌试验效果初探[J].西北林学院学报,2008,23(06):118.
　WEN Xiaoping,HUANG Baoling,LV Chengqun,et al.Research for the Effect of Rhizobia Inoculation on the Eucalyptus g randis×E. urophylla[J].JOURNAL OF NORTHWEST FORESTRY UNIVERSITY,2008,23(05):118.
[2]佘汉基,蔡金桓,薛立*,等. 模拟外源性氮磷对尾叶桉和马占相思混合凋落叶分解的影响[J].西北林学院学报,2017,32(6):45.[doi:10.3969/j.issn.1001-7461.2017.06.07]
　SHE Han-ji,CAI Jin-huan,XUE Li*,et al. Effects of Nitrogen and Phosphorus Additions on the Decomposition of Mixed Leaf Litter in Eucalyptus urophylla and Acacia mangium Plantations[J].JOURNAL OF NORTHWEST FORESTRY UNIVERSITY,2017,32(05):45.[doi:10.3969/j.issn.1001-7461.2017.06.07]

备注/Memo

备注/Memo:
收稿日期20060224修回日期20060316基金项目广西自然科学基金项目(0448005)作者简介韦原莲（1965），女，广西上林人，实验师，主要从事林业方面的研究。
更新日期/Last Update: 2010-05-04