[1]曲玲,曹有龙,侯玉霞*,等.将GNA基因导入宁夏枸杞及其表达的研究[J].西北林学院学报,2007,22(03):88-91.
　QU Ling,CAO Youlong,HOU Yuxia,et al.A Study of the Introduction and Expression of GNA Gene in Lycium bararum L.[J].JOURNAL OF NORTHWEST FORESTRY UNIVERSITY,2007,22(03):88-91.
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将GNA基因导入宁夏枸杞及其表达的研究()
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《西北林学院学报》[ISSN:1001-7461/CN:61-1202/S]

卷:
第22卷
期数:
2007年03期
页码:
88-91
栏目:
林木繁育与栽培
出版日期:
2007-06-30

文章信息/Info

Title:
A Study of the Introduction and Expression of GNA Gene in Lycium bararum L.
作者:
曲玲1; 2; 曹有龙2; 侯玉霞1*; 吴家和3; 罗青2; 田颖川3
1.中国农业大学 理学院，北京 100094；2.宁夏农林科学院，宁夏 银川 750002；3.中国科学院 微生物研究所，北京 100080
Author(s):
QU Ling1; 2; CAO Youlong2; HOU Yuxia1; WU Jiahe3; LUO Qing2; Tian Yingchuan3
1.College of Science, China Agricultural University, Beijing 100095, China; 2.Ningxia Academy of Agricultural and Forestry Sciences, Yinchuan,Ningxia 750002, China; 3.Institute of Microbiology, Chinese Academy of Sciences, Beijing 100080, China
关键词:
宁夏枸杞; 雪花莲凝集素; CoYMV启动子; 农杆菌介导遗传转化; Western blot杂交
Keywords:
Lycium bararum; Galanthus nivalis agglutinin(GNA);  CoYMV promoter;  Agrobacteriummediated transformation;  Western blot analysis
分类号:
S793.904
文献标志码:
A
摘要:
为了解决枸杞蚜虫侵害的难题，以宁夏枸杞主栽品种“宁杞1号”叶片为外植体，通过农杆菌介导的转化方法，将改造后的雪花莲凝集素GNA基因转入枸杞，以抗性愈伤组织诱导率作为转化效率的指标，对影响转化效率的因素进行了研究，初步建立了转化频率较高的转化系统。对获得的70个转基因株系进行PCR检测，阳性率为60%。运用Western blot杂交检测了2个转化子叶片和果实中GNA的表达，结果表明,其中一个转化株系在维管组织成分较高的叶片、青果中得到了GNA的表达，而在维管束成分极少的红果中未检测到GNA蛋白的表达。
Abstract:
A modified Galanthus nivalis agglutinin (GNA) gene was introduced into the genome of main cultivar 'Ningqi No.1' of Lycium bararum in vitro with explants of leaf disks mediated by Agrobacterium tumefaciens. Several factors affecting transformation efficiencies were studied based on induction rate of resistant callus. An effective system for genetic transformation of Lycium bararum was set up, and 70 kanamycinresistant transformants were obtained. Results of PCR indicated that 60% kana resistant plants could be detected the gna gene. Western blot analysis of leaf and fruit proteins from two PCR positive and aphid resistant transformants was carried out. In one of transformants, the GNA protein was detected in leaves and green fruits where the vascular tissues occupies a relatively larger part of the organs, but in red fruits where the vascular tissues occupy few parts of the organs the GNA protein wasn't detected.

参考文献/References:

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备注/Memo

备注/Memo:
收稿日期：20061212修回日期：20070125基金项目：宁夏回族自治区自然科学基金项目（B1006）作者简介：曲玲（1972），女，宁夏银川人，助理研究员，在读硕士研究生，研究方向为农药分子生物学。*通讯作者：侯玉霞。
更新日期/Last Update: 2010-05-04