[1]王宏,赵辉,李周岐,等.杜仲RAPD反应体系的优化[J].西北林学院学报,2007,22(04):86-89.
　WANG Hong,ZHAO Hui,LI Zhou-qi,et al.Optimization of RAPD Reaction System in Eucommia ulmoides Olive.[J].JOURNAL OF NORTHWEST FORESTRY UNIVERSITY,2007,22(04):86-89.
点击复制

杜仲RAPD反应体系的优化 ()
分享到：

《西北林学院学报》[ISSN:1001-7461/CN:61-1202/S]

卷:
第22卷
期数:
2007年04期
页码:
86-89
栏目:
水土保持与沙漠治理
出版日期:
2007-08-30

文章信息/Info

Title:
Optimization of RAPD Reaction System in Eucommia ulmoides Olive.
作者:
王宏; 赵辉; 李周岐; 张康健; 辛福梅
西北农林科技大学,林学院,陕西,杨陵,712100
Author(s):
WANG Hong; ZHAO Hui; LI Zhou-qi; ZHANG Kang-jian; Xin Fu-mei
关键词:
杜仲;  RAPD;  体系优化
分类号:
S722.31
文献标志码:
A
摘要:
以杜仲优良品种的幼叶为材料提取总DNA,采用单因素梯度试验筛选法进行了杜仲RAPD反应的优化.确定的优化反应体系为:25 μL反应体积中,10×buffer 2.5 μL,TAqDNA聚合酶1 U,随机引物(5 μmol/L)2.0 μL,MgCl2(25 mmol/L)2.0 μL,dNTP(2.5 mmol/L)2.0 μL,模板2 mg/L,补ddH2O至25 μL.扩增程序为:94 ℃预变性5 min,94 ℃变性30 s,37 ℃退火30 s,72 ℃延伸90 s,35个循环,72 ℃延伸10 min,4 ℃保持产物.在此条件下得到的RAPD图谱清晰、稳定,为进一步利用该技术开展杜仲有关分子生物学研究提供了技术参考.

相似文献/References:

[1]何 泼,任红媛,申 延.乙酸对杜仲愈伤组织胶含量的影响[J].西北林学院学报,2007,22(02):149.
　HE Po,REN Hong-yuan,SHEN Yan.Effects of Adding Acetic Acid on the Rubber Content in the Callus of Eucommia ulmoides[J].JOURNAL OF NORTHWEST FORESTRY UNIVERSITY,2007,22(04):149.
[2]樊光辉.40个杜仲无性系嫁接成活率与接芽萌发枝高生长量的比较试验[J].西北林学院学报,2008,23(03):91.
　FAN Guanghui.A Comparative Study on the Gratification and Survival Rates 40 Clone s of Eucommia ulmoides[J].JOURNAL OF NORTHWEST FORESTRY UNIVERSITY,2008,23(04):91.
[3]樊光辉.40个杜仲无性系嫁接成活率与接芽萌发枝高生长量的比较试验[J].西北林学院学报,2008,23(02):91.
　FAN Guanghui.A Comparative Study on the Gratification and Survival Rates 40 Clone s of Eucommia ulmoides[J].JOURNAL OF NORTHWEST FORESTRY UNIVERSITY,2008,23(04):91.
[4]李俊红,张焕玲,李周岐.杜仲组织培养再生体系的优化[J].西北林学院学报,2008,23(04):97.
　LI Jun hong,ZHANG Huan ling,LI Zhou qi*.ptimization of Tissue Culture Regeneration System of Eucommia ulmoides Oliv.[J].JOURNAL OF NORTHWEST FORESTRY UNIVERSITY,2008,23(04):97.
[5]张京芳,张康健,张小平.杜仲红枣复合饮料加工工艺研究[J].西北林学院学报,2000,15(01):60.
　Zhang Jingfang,Zhang Kangjian,Zhang Xiaoping.Processing Technology of A Compound Beverage of Duzhong and Jujube[J].JOURNAL OF NORTHWEST FORESTRY UNIVERSITY,2000,15(04):60.
[6]董娟娥,马柏林,仝小林,等.提高杜仲叶中主要活性物质提取率的研究[J].西北林学院学报,2002,17(01):64.
　A Study on the Extraction of the Bioactive Constituents in the Leaves of Eucommia ulmoides[J].JOURNAL OF NORTHWEST FORESTRY UNIVERSITY,2002,17(04):64.
[7]董娟娥,马柏林,张康健,等.杜仲籽油中α-亚麻酸的含量及其生理功能[J].西北林学院学报,2002,17(02):73.
　Contents and Active Function of α-linolenic in Eucommia ulmoides Seed Oil[J].JOURNAL OF NORTHWEST FORESTRY UNIVERSITY,2002,17(04):73.
[8]弓弼,马惠玲,沈杰.杜仲优树的组培繁殖技术[J].西北林学院学报,2002,17(04):38.
　Tissue Culture of Superior Eucommia Clone[J].JOURNAL OF NORTHWEST FORESTRY UNIVERSITY,2002,17(04):38.
[9]徐怀德,张康健,董娟娥,等.杜仲茶饮料加工技术研究[J].西北林学院学报,2003,18(03):69.
　On Processing Technology of Eucommia ulmoides Tea Beverage[J].JOURNAL OF NORTHWEST FORESTRY UNIVERSITY,2003,18(04):69.
[10]张博勇,张康健,王亚琴,等.杜仲优良种源区与类型选择的研究[J].西北林学院学报,2003,18(04):32.
　The Selection of Fine Germplasm Regions and Variant Types of Eucommia[J].JOURNAL OF NORTHWEST FORESTRY UNIVERSITY,2003,18(04):32.
[11]杨恩让,王 宏,李 煜.杜仲优良品种及无性系DNA指纹图谱的构建[J].西北林学院学报,2013,28(05):100.[doi:10.3969/j.issn.1001-7461.2013.05.19]
　YANG Enrang,WANG Hong,LI Yu.DNA Fingerprinting of Eucommia ulmoides Cultivars and Clones Using RAPD Markers[J].JOURNAL OF NORTHWEST FORESTRY UNIVERSITY,2013,28(04):100.[doi:10.3969/j.issn.1001-7461.2013.05.19]

备注/Memo

备注/Memo:
国家林业局科技推广项目([2004]-26-2)
更新日期/Last Update: 2010-04-29