[1]周玮,李周岐,李煜,等.杜仲种子无菌苗的获得及染色体加倍技术研究[J].西北林学院学报,2011,26(04):132-136.
点击复制

杜仲种子无菌苗的获得及染色体加倍技术研究()
分享到：

《西北林学院学报》[ISSN:1001-7461/CN:61-1202/S]

卷:
第26卷
期数:
2011年04期
页码:
132-136
栏目:
林木遗传育种与培育
出版日期:
2011-07-30

文章信息/Info

作者:
周玮;李周岐;李煜;孙勇;
西北农林科技大学林学院;宝鸡职业技术学院生物工程系;
关键词:
杜仲; 种子; 无菌苗; 染色体加倍; 多倍体
文献标志码:
A
摘要:
研究了杜仲种子试管苗萌发和无菌苗培育技术,以及种子染色体加倍技术,提出了以种子为材料通过试管萌发进行染色体加倍的技术方法:杜仲成熟种子剥去种皮,用清水反复冲洗,经500 mg.L-1的赤霉素浸泡24 h后,置于浓度为0.3%的秋水仙素中浸泡48 h,再于70%酒精消毒30 s,1 g.L-1HgCl2表面消毒12 min,无菌水冲洗3~4次,切去少许胚芽和胚根端胚乳后胚根向下接入无激素的MS培养基中培养。在组织培养条件下,种子的萌发率最高可达100%,且无菌苗生长良好;多倍体诱变率最高达到76.09%。本试验最终获得多倍体变异植株424株。

相似文献/References:

[1]何 泼,任红媛,申 延.乙酸对杜仲愈伤组织胶含量的影响[J].西北林学院学报,2007,22(02):149.
　HE Po,REN Hong-yuan,SHEN Yan.Effects of Adding Acetic Acid on the Rubber Content in the Callus of Eucommia ulmoides[J].JOURNAL OF NORTHWEST FORESTRY UNIVERSITY,2007,22(04):149.
[2]蔡琰琳,金则新＊.濒危植物夏蜡梅果实、种子形态变异研究⒇[J].西北林学院学报,2008,23(03):44.
　CAI Yanlin,JI N Zexi n.Morphological Variation of Fruits and Seeds in Endangered Plant Sinocalycanthus chinensis[J].JOURNAL OF NORTHWEST FORESTRY UNIVERSITY,2008,23(04):44.
[3]张香凝,孙向阳ˇ,王保平,等.种子发芽对水分胁迫的响应[J].西北林学院学报,2008,23(03):36.
　ZHANG Xiangning,SUN Xiangyang,WANG Baoping,et al.Response of Larrea tridentata Seed Germination to Water Stress[J].JOURNAL OF NORTHWEST FORESTRY UNIVERSITY,2008,23(04):36.
[4]樊光辉.40个杜仲无性系嫁接成活率与接芽萌发枝高生长量的比较试验[J].西北林学院学报,2008,23(03):91.
　FAN Guanghui.A Comparative Study on the Gratification and Survival Rates 40 Clone s of Eucommia ulmoides[J].JOURNAL OF NORTHWEST FORESTRY UNIVERSITY,2008,23(04):91.
[5]张香凝,孙向阳ˇ,王保平,等.种子发芽对水分胁迫的响应[J].西北林学院学报,2008,23(02):36.
　ZHANG Xiangning,SUN Xiangyang,WANG Baoping,et al.Response of Larrea tridentata Seed Germination to Water Stress[J].JOURNAL OF NORTHWEST FORESTRY UNIVERSITY,2008,23(04):36.
[6]樊光辉.40个杜仲无性系嫁接成活率与接芽萌发枝高生长量的比较试验[J].西北林学院学报,2008,23(02):91.
　FAN Guanghui.A Comparative Study on the Gratification and Survival Rates 40 Clone s of Eucommia ulmoides[J].JOURNAL OF NORTHWEST FORESTRY UNIVERSITY,2008,23(04):91.
[7]李俊红,张焕玲,李周岐.杜仲组织培养再生体系的优化[J].西北林学院学报,2008,23(04):97.
　LI Jun hong,ZHANG Huan ling,LI Zhou qi*.ptimization of Tissue Culture Regeneration System of Eucommia ulmoides Oliv.[J].JOURNAL OF NORTHWEST FORESTRY UNIVERSITY,2008,23(04):97.
[8]张京芳,张康健,张小平.杜仲红枣复合饮料加工工艺研究[J].西北林学院学报,2000,15(01):60.
　Zhang Jingfang,Zhang Kangjian,Zhang Xiaoping.Processing Technology of A Compound Beverage of Duzhong and Jujube[J].JOURNAL OF NORTHWEST FORESTRY UNIVERSITY,2000,15(04):60.
[9]董娟娥,马柏林,仝小林,等.提高杜仲叶中主要活性物质提取率的研究[J].西北林学院学报,2002,17(01):64.
　A Study on the Extraction of the Bioactive Constituents in the Leaves of Eucommia ulmoides[J].JOURNAL OF NORTHWEST FORESTRY UNIVERSITY,2002,17(04):64.
[10]董娟娥,马柏林,张康健,等.杜仲籽油中α-亚麻酸的含量及其生理功能[J].西北林学院学报,2002,17(02):73.
　Contents and Active Function of α-linolenic in Eucommia ulmoides Seed Oil[J].JOURNAL OF NORTHWEST FORESTRY UNIVERSITY,2002,17(04):73.

备注/Memo

备注/Memo:
西安市科技计划项目(NC08009);;
更新日期/Last Update: 2011-05-20