[1]任振华,杨途熙 *,魏安智,等.RP-HPLC测定花椒中吴茱萸次碱的含量[J].西北林学院学报,2013,28(06):108-111.[doi:10.3969/j.issn.1001-7461.2013.06.21]
　REN Zhen-hua,YANG Tu-xi*,WEI An-zhi,et al.Determination of Rutaecarpine in Zanthoxylum bungeagum by RP-HPLC[J].JOURNAL OF NORTHWEST FORESTRY UNIVERSITY,2013,28(06):108-111.[doi:10.3969/j.issn.1001-7461.2013.06.21]
点击复制

RP-HPLC测定花椒中吴茱萸次碱的含量()
分享到：

《西北林学院学报》[ISSN:1001-7461/CN:61-1202/S]

卷:
第28卷
期数:
2013年06期
页码:
108-111
栏目:
出版日期:
2013-11-30

文章信息/Info

Title:
Determination of Rutaecarpine in Zanthoxylum bungeagum by RP-HPLC
文章编号:
1001-7461(2013)06-0108-04
作者:
任振华1; 杨途熙 2*; 魏安智2; 王 媛2; 张艳军1; 冯世静2
1.西北农林科技大学 生命科学学院,陕西 杨陵 712100;
2.西北农林科技大学 林学院,陕西 杨陵 712100
Author(s):
REN Zhen-hua1;  YANG Tu-xi2*;  WEI An-zhi2;  WANG Yuan2; ZHANG Yan-jun1;  FENG Shi-jing2
1.college of Life Sciences, Northwest A&F University, Yangling, Shaanxi 712100, China;
2. College of Forestry,Northwest A&F University, Yangling, Shaanxi 712100,China
关键词:
花椒;  RP-HPLC;  吴茱萸次碱;  含量测定
Keywords:
Zanthoxylunm bungeanum;  RP-HPLC;  rutaecarpine;  content determination
分类号:
S789.5
DOI:
10.3969/j.issn.1001-7461.2013.06.21
文献标志码:
A
摘要:
探讨花椒中吴茱萸次碱含量的RP-HPLC测定条件,比较不同种源花椒中吴茱萸次碱的含量差异。结果表明,吴茱萸次碱的线性范围为14.4～27.0 μg·mL-1(r=0.999 5),平均回收率为98.63%(RSD=1.73%, n=5)。不同种源花椒中吴茱萸次碱的平均含量分别为:野花椒 24.050 μg·mL-1(RSD=1.77%, n=3); 凤县大红袍 23.677 μg·mL-1(RSD=4.94%, n=3); 韩城大红袍 18.869 μg·mL-1(RSD=3.00%, n=3); 秦安1号 17.173 μg·mL-1(RSD=2.45%, n=3)。RP-HPLC法测定吴茱萸次碱含量简便可靠,可作为花椒中吴茱萸次碱的快速测定方法。
Abstract:
Conditions of HPLC to measure the contents of rutaecarpine in Zanthoxylum bungeagum from different provenances were established, and the rutaecarpine contents were compared. The results showed that the linear range of rutaecarpine was 14.4 to 27.0 μg·mL-1(r=0.999 5). The average recovery rate was 98.63%(RSD=1.73%, n=5). The average contents of rutaecarpine in Zanthoxylum bungeagum, Zanthoxylum simulans, cultivars of Fengxiandahongpao, Hanchengdahongpao and Qin’anyihao were 24.050 μg·mL-1(RSD=1.77%, n=3); 23.677 μg·mL-1(RSD=4.94%,n=3); 18.869 μg·mL-1(RSD=3.00%,n=3); 17.173 μg·mL-1(RSD=2.45%, n=3), respectively. It was concluded that the measurement of rutaecarpine by RP-HPLC was simple, rapid and accurate, and which could be used as a routine method to measure this compound.

参考文献/References:

[1] 吴素蕊,阚建全,刘春芬. 花椒的活性成分与应用研究[J].中国食品添加剂,2004(2):75-78.
[2] 袁娟丽,王四旺.花椒的化学成分及其药效学研究[J].现代生物医学进展,2010,10(3):552-554.
[3] 孙小文,段志兴. 花椒属药用植物研究进展[J].药学学报,1996,31(3):231-240.
[4] 石雪萍,关荣琴,张鸣镝,等.花椒属植物生物碱研究进展[J].中国野生植物资源,2010,25(4):1-7.
[5] 余奇,郭澄,程泽能.吴茱萸次碱的研究进展[J].药学实践杂志,2007,25(6):353-357.
[6] SHEEN W S,TSAI I L,TENG C M,et al. Indolopyridoquinazoline alkaloids with antiplatelet aggregation activity from Zanthoxylum integrifoliolum[J]. Planta medica,1996,62(2):175-176.
[7] 朱红枚.野花椒化学成分的研究[D].成都:西南交通大学2007.
[8] 邱模昌,彭维杰.吴茱萸次碱药理作用研究进展[J].科技信息,2012(1):651-652.
[9] 王君伟,李小定,吴媛瑾,等.吴茱萸中吴茱萸次碱提取工艺及响应面分析法的优化[J].湖北农业科学,2009, 48(1):175-178.
[10] 李景侠,赵建民,杨勇辉.花椒的采收贮藏与干制技术[J].中国林副特产,1999(4):21-22.
[11] 王如平,石雪萍,张卫明,等.花椒总生物碱提取条件研究[J].食品科学, 2008.29(10):273-276.WANG R P,SHI X P,ZHANG W M,et al.Study one extraction technology of total alkaloids from Zanthoxylum bungeanum[J]. Food Science, 2008.29(10):273-276.(in Chinese)
[12] 陶 清,周兴旺,吕鉴泉. 正交试验法优化吴茱萸总碱的微波提取[J].天然产物研究与开发,2007(19): 494-498.
[13] 甄攀,张荣山,白雪梅,等.吴茱萸中吴茱萸碱和吴茱萸次碱的提取条件研究[J].中国药学杂志,2005,40(2): 99-101.
[14] 谢娟,张启云,余日跃,等.吴茱萸水提物中吴茱萸碱和吴茱萸次碱的高效液相色谱含量测定[J].时珍国医国药,2008,19(7): 1570-1572.
[15] 沈晓霞,盛束军,徐建中.环境因子对益母草总生物碱含量的影响[J].浙江农业学报,2002,14(4):221-225.SHEN X X, SHENG S J, XU J Z. Effect of environmental factors on the total alkaloid content in Yimucao[J].Acta Agriculturae Zhejiangensis,2002,14(4):221-225.(in Chinese)
[16] 陈军,丁美海,胡榜文,等. 不同光色对黄连生物碱含量的影响[J].西北林学院学报,2011,26(6):114-118.CHEN J,DING M H,HU B W,et al. Effects of different chromatic lights on contents of alkaloids in Coptis chinesis[J].Journal of Northwest Forestry University, 2011,26(6):114-118.(in Chinese)

相似文献/References:

[1]陈 艳,李广泽*,曹雪峰,等.RP-HPLC法同时测定沙地柏中3种鬼臼毒素类化合物的含量[J].西北林学院学报,2007,22(02):127.
　CHEN Yan,LI Guang-ze*,et al.Quantitative Determination of the Podophyllotoxin Analogues in Sabina vulgaris by RP-HPLC[J].JOURNAL OF NORTHWEST FORESTRY UNIVERSITY,2007,22(06):127.
[2]陈 丹,曹支敏,王培新.花椒落叶病寄主抗病性及其病原菌生物学特性[J].西北林学院学报,2008,23(06):126.
　CHEN Dan,CAO Zhimin,WANG Peixin.The Host′s Resistance to Zanthoxylum bungeanum Disease and Pathogen′ s Biological Characteristics Defoliation Disease[J].JOURNAL OF NORTHWEST FORESTRY UNIVERSITY,2008,23(06):126.
[3]吴佳,孙丙寅,王敏.地面覆盖对花椒林生理生态效应的影响[J].西北林学院学报,2005,20(04):27.
　WU Jia,SUN Bing-yin,WANG Min.The Ecological and Physiological Effect of Different Ground Covering in Zanthoxylum bungeanum Forest[J].JOURNAL OF NORTHWEST FORESTRY UNIVERSITY,2005,20(06):27.
[4]孟庆翠,刘淑明,孙丙寅.配方施肥对花椒产量的影响[J].西北林学院学报,2009,24(03):105.
　MENG Qing-cui,LIU Shu-ming,SUN Bing-yin.The Effect of Balanced Fertilization on Yield of Zanthoxylum bungeanum[J].JOURNAL OF NORTHWEST FORESTRY UNIVERSITY,2009,24(06):105.
[5]原双进,张振南,王云芳,等.花椒良种选育研究[J].西北林学院学报,2006,21(02):84.
　YUAN Shuangjin{},ZHANG Zhennan,WANG Yunfang,et al.On Selecting and Breeding for Superior Varieties of Zanthoxylum bungeanum[J].JOURNAL OF NORTHWEST FORESTRY UNIVERSITY,2006,21(06):84.
[6]樊鸿章,雷淑彦.韩城南部花椒主产区土壤环境质量评价[J].西北林学院学报,2007,22(06):40.
　FAN Hongzhang,LEI Shuyan.Assessment of Soil Environment Quality of Zanthoxylum bungeanum in the South of Hancheng[J].JOURNAL OF NORTHWEST FORESTRY UNIVERSITY,2007,22(06):40.
[7]邓振义,孙丙寅,康克功,等.花椒嫩芽主要营养成分的分析[J].西北林学院学报,2005,20(01):179.
　Analysis of the Main Nutritional Labeling in the Tender Bud of Zanthoxylum bungeanum[J].JOURNAL OF NORTHWEST FORESTRY UNIVERSITY,2005,20(06):179.
[8]郑海星,李周岐,薛惠丹,等.花椒种质资源的RAPD分析[J].西北林学院学报,2011,26(02):96.
[9]曹支敏,明玉玲,陈丹,等.花椒干腐病的寄主抗病性与病原菌致病性分化[J].西北林学院学报,2010,25(06):115.
[10]刘杜玲,王晓燕,吴顺科,等.不同种源花椒抗旱性研究[J].西北林学院学报,2010,25(06):25.

备注/Memo

备注/Memo:
收稿日期:20013-03-26 修回日期:2013-05-27
基金项目:国家林业局推广项目([2011]24); 西北农林科技大学科技大学农业推广基金(TGZX2012-07)。
作者简介:任振华,男,在读硕士,研究方向:生物多样性与资源利用。E-mail:xn_trypsin@163.com
*通信作者:杨途熙,男,副教授,研究方向:生物多样性与资源利用。E-mail:y2848@126.com
更新日期/Last Update: 2013-11-30