[1]李 薇,刘春英,郭 娟,等.3个美洲黑杨新无性系的SSR分析及鉴定[J].西北林学院学报,2014,29(01):73-77.[doi:10.3969/j.issn.1001-7461.2014.01.15]
　LI Wei,LIU Chun-ying,GUO Juan,et al.Analysis and Identification of Three New Clones of Populus deltoides Based on SSR Molecular Markers[J].JOURNAL OF NORTHWEST FORESTRY UNIVERSITY,2014,29(01):73-77.[doi:10.3969/j.issn.1001-7461.2014.01.15]
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3个美洲黑杨新无性系的SSR分析及鉴定()
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《西北林学院学报》[ISSN:1001-7461/CN:61-1202/S]

卷:
第29卷
期数:
2014年01期
页码:
73-77
栏目:
林木遗传育种与培育
出版日期:
2014-01-21

文章信息/Info

Title:
Analysis and Identification of Three New Clones of Populus deltoides Based on SSR Molecular Markers
文章编号:
1001-7461(2014)01-0073-05
作者:
李 薇1; 刘春英1; 郭 娟1; 王永进2; 樊军锋1*
1.西北农林科技大学 林学院,陕西 杨陵 712100;
2.岚皋县林业技术推广服务中心,陕西 岚皋 725400
Author(s):
LI Wei1;  LIU Chun-ying1; GUO Juan1;  WANG Yong-jin2; FAN Jun-feng1*
1.College of Forestry, Northwest A&F University, Yangling, Shaanxi 712100, China;
2.Forestry Technology Popularization Service Center of Langao County,Langao,Shaanxi 725400,China
关键词:
美洲黑杨;  SSR;  品种鉴定;  遗传多态性
Keywords:
Populus deltoides;  SSR;  cultivar identification;  genetic polymorphism
分类号:
S792.119
DOI:
10.3969/j.issn.1001-7461.2014.01.15
文献标志码:
A
摘要:
利用SSR-PCR的方法对6个美洲黑杨(Populus deltoides)无性系进行了基因组多态性分析。从30对引物中筛选出10对特异性引物并进行PCR扩增,共扩增出了132个位点,其中多态性位点125个,所占比率为94.69%,基因型间的平均遗传距离为0.45。聚类分析结果表明,各杨树无性系间存在一定的遗传差异,准确的反映了遗传差异对外部形态的反应。
Abstract:
Simple sequence repeat(SSR)was used to determine the genomic DNA variations and genetic relationships in Populus deltoides.Six cultivars were used in SSR analysis.A total of 10 SSR primers which were polymorphic and informative were selected to determine the genetic diversity and genetic relationships.One hundred and thirty two DNA bands were amplified,125 of which were polymorphic(94.69%).The average genetic distance between genotypes was 0.45.The cluster analysis demonstrated the existence of genetic differences between varieties of Populus,accurately reflecting the genetic differences in response to the external morphology of the reaction.

参考文献/References:

[1] 李世峰,张博,陈英,等.美洲黑杨种质资源遗传多样性的SSR分析[J].南京林业大学学报:自然科学版,2006,30(4): 10-14. LI S F,ZHANG B,CHEN Y,et al.Analusis of genetic diversity of Populus deltoides germplasm by SSR[J].Journal of Nanjing Forestry Universtity,2006,30(4):10-14.(in Chinese)
[2] 张德强,张志毅,杨凯.杨树分子标记研究进展[J].北京林业大学学报,2000,22(6): 79-84.ZHANG D Q,ZHANG Z Y,YANG K.Advances of molecular marker researches in poplar[J].Journal of Beijing Forestry University,2000,22(6): 79-84.(in Chinese)
[3] 王辉,杨敏生,朱建峰.利用SSR对杨属部分种及杂种的分析鉴定[J].东北林业大学学报,2008,36(12):4-6. WANG H,YANG M S,ZHU J F.SSR analysis of some species and hybrids in Populus[J].Journal of Northesat Forestry University,2008,36(12):4-6.(in Chinese)
[4] 梁海永,刘彩霞,刘兴菊,等.杨树品种的 SSR 分析及鉴定[J].河北农业大学学报,2005,28(7): 27-31. LIANG H Y,LIU C X,LIU X J,et al.Simple sequence repeat(SSR)analysis and identify of different cultivars in Populus L.[J].Journal of Agricultural University of Hebei,2005,28(7):27-31.(in Chinese)
[5] MORGANTE M,OLIVIERI A M.PCR-amplified microsatellites as markers in plant genetics[J].The Plant Journal,1993,3(1): 175-182.
[6] POWELL W,MORGANTE M,ANDRE C,et al.Hypervariable microsatellites provide a general source of polymorphic DNA markers for the chloroplast genome[J].Current Biology,1995,5(9): 1023-1029.
[7] WEBER J L,MAY P E.Abundant class of human DNA polymorphisms which can be typed using the polymerase chain reaction[J].American Journal of Human Genetics,1989,44(3): 388.
[8] 王忠华.DNA指纹图谱技术及其在作物品种资源中的应用[J].分子植物育种,2006,4(3): 425-430.WANG Z H.DNA fingerprinting technology and its application in crop germplasm resources[J].Molecular Plant Breeding,2006,4(3):425-430.(in chinese)
[9] 王琼,郑勇奇,周建仁.分子标记在林业植物新品种鉴别中的应用及前景[J].林业科学,2008,44(6):180-182.WANG Q,ZHENG Y Q,ZHOU J R.Application of molecular markers in identification of new varietices in forest plants[J].Scientia Silvar Sinicae,2008,44(6):180-182.(in Chinese)
[10] 巩艳红,刘军,张健,等.毛豹皮樟的叶片DNA提取及其RAPD引物筛选[J].西北林学院学报,2004,19(4):35-37.GONG Y H,LIU J,ZHANG J,et al.DNA extraction and RAPD primer screening in leaves of Litsea coreana var.lanuginosa[J].Jounml of Northwest Forestry University,2004,19(4):35-37.(in Chinese)
[11] SCHOOT J D,POSPíKOVá M,VOSMAN B.Development and characterization of microsatellite markers in black poplar(Populus nigra L.)[J].Theoretical and Applied Genetics,2000,101(1-2): 317-322.
[12] TUSKAN G A,GUNTER L E,YANG Z K,et al.Characterization of microsatellites revealed by genomic sequencing of Populus trichocarpa[J].Canadian Journal of Forest Research,2004,34(1): 85-93.
[13] SMULDERS M J M,VAN DER SCHOOT J,ARENS P,et al.Trinucleotide repeat microsatellite markers for black poplar(Populus nigra L.)[J].Molecular Ecology Notes,2001,1(3): 188-190.
[14] PAOLUCCI I,GAUDET M,JORGE V,et al.Genetic linkage maps of Populus alba L.and comparative mapping analysis of sex determination across Populus species[J].Tree Genetics & Genomes,2010,6(6): 863-875.
[15] 王竹林,杨睿,刘联正,等.聚丙烯酰胺凝胶电泳银染的影响因素[J].实验室研究与探索,2011,30(9): 15-17.WANG Z L,YANG R,LIU L Z,et al.Factors of silver staining of polyacrylamide gel electrophoresis[J].Research and Exploration in Laboratory,2011,30(9):15-17.(in Chinese)
[16] 张志清,郑有良,魏育明,等.SSR 标记遗传距离与小麦杂种优势相关分析[J].四川农业大学学报,2003,21(1): 6-9. ZHANG Z Q,ZHENG Y L,WEI Y M,et al.Relationship between heterosis and genetic distance based on simple sequence repeat markers in wheat[J].Journal of Sichuan Agricultural University,2003,21(1):6-9.(in Chinese)
[17] IMBERT E,LEFEVRE F.Dispersal and gene flow of Populus nigra (Salicaceae)along a dynamic river system[J].Journal of Ecology,2003,91(3): 447-456.
[18] RAHMAN,M H,RAJORA O P.Microsatellite DNA somaclonal variation in micropropagated trembling aspen(Populus tremuloides)[J].Plant Cell Reports,2001,20(6): 531-536.
[19] SARAO N K,VIKAL Y,SINGH K,et al.SSR marker-based DNA fingerprinting and cultivar identification of rice(Oryza sativa L.)in Punjab state of India[J].Plant Genetic Resources,2010,8(01): 42-44.
[20] 刘春英,樊军锋,高建社,等.杨树新杂种的SSR分析及鉴定[J].西北林学院学报,2013,28(2):70-73.LIU C Y,FAN J F,GAO J S,et al.Simple sequence repeat analysis and identification of new Populuss hybrid[J].Jounal of Northwest Forestry University,2013,28(2):70-73.(in Chinese)
[21] 黎云昆.植物新品种保护与种植业产业的发展[J].西北林学院学报,2003,18(1): 104-108. LI Y K.Protection of new varieties and development of growing insustry[J].Journal of Northwest Forestry University,2003,18(1):104-108.(in Chinese)

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备注/Memo

备注/Memo:
收稿日期:2013-04-11 修回日期:2013-05-07
基金项目:“十二五”农村领域国家科技计划专题“超高产优质杨树速生林新品种选育”(2012BAD01B03)。
作者简介:李薇,女,硕士研究生,研究方向:林木遗传育种。E-mail:lwzcc1987@163.com
*通信作者:樊军锋,男,研究员,研究方向:林木遗传育种。E-mail: fanjf28@163.com
更新日期/Last Update: 2014-01-20