[1]李薇,徐华,樊军锋*. 带有GFP标签的油松苯丙氨酸解氨酶基因表达载体的构建[J].西北林学院学报,2014,29(05):84-87.[doi:doi:10.3969/j.issn.1001-7461.2014.05.15]
　LI Wei,XU Hua,FAN Jun-feng*. Construction of GFP Tagged Phenylalanine Ammonialyase Gene of Pinus tabulaeformis Expression Vector[J].JOURNAL OF NORTHWEST FORESTRY UNIVERSITY,2014,29(05):84-87.[doi:doi:10.3969/j.issn.1001-7461.2014.05.15]
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 带有GFP标签的油松苯丙氨酸解氨酶基因表达载体的构建()
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《西北林学院学报》[ISSN:1001-7461/CN:61-1202/S]

卷:
第29卷
期数:
2014年05期
页码:
84-87
栏目:
出版日期:
2014-09-30

文章信息/Info

Title:
 Construction of GFP Tagged Phenylalanine Ammonialyase Gene of Pinus tabulaeformis Expression Vector
文章编号:
1001-7461(2014)05-0084-04
作者:
 李薇1; 徐华2; 樊军锋1*
 （1.西北农林科技大学 林学院，陕西 杨陵 712100；2.陕西省林业厅 种苗工作站，陕西 西安 710082）
Author(s):
 LI Wei1; XU Hua2; FAN Jun-feng1*
 (1. College of Forestry, Northwest A & F University,Yangling,Shaanxi 712100,China;2.Tree and Seed Workstation of Department of Forest of Shaanxi Province,Xi’an, Shaanxi 710082,China)
关键词:
 ; 苯丙氨酸解氨酶基因; 植物表达载体; 基因重组; 农杆菌; 绿色荧光蛋白
Keywords:
 ; phenylalanin ammonialyase;  plant expression vector;  gene recombination technology;  agrobacterium;  GFP
分类号:
S791.254
DOI:
doi:10.3969/j.issn.1001-7461.2014.05.15
文献标志码:
A
摘要:
 利用基因重组技术，将油松的苯丙氨酸解氨酶基因（TaPAL）克隆到PBI121植物表达载体中，并且用报告基因GFP替换了GUS，构建了包含绿色荧光蛋白(GFP)基因的植物重组表达载体PBI121-GFP-TaPAL,GFP标签比GUS基因更加易于检测，方便进行TaPAL功能鉴定方面的研究。利用电击法将重组表达载体成功的转化进农杆菌GV3301中。该研究可以为深入研究苯丙氨酸解氨酶的结构及功能及进一步通过导入次生代谢关键酶来提高植物的抗病育种提供基础。
Abstract:
 The research aim of this study was to transfer the Phenylalanin ammonialyase of Pinus tabulaeformis into plants expression vector PBI121 by using the gene recombination technology, and by replacing report gene GUS with GFP. We constructed the recombinant expression plasmid PBll21-GFP-TaPAL which contained the green fluorescent protein gene. Compared to GUS gene GFP labels was easier to detect and identify the TaPAL function. By using the electric shock method we transformed the recombinated expression vector into agrobacterium GV3301. The research provided the basis for further study of the structure and function of phenylalanine ammonialyase and by importing the key secondary metabolism enzymes to improve the plant disease resistance.

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备注/Memo

备注/Memo:
 收稿日期：2014-01-09修回日期：2014-03-07
基金项目：油松高世代育种材料的创造测定及选择研究；林业公益性行业科研专项（200704039）。
作者简介：李薇，女，在读硕士，研究方向：林木遗传育种。E-mail：lwzcc1987@163.com
*通信作者：樊军锋，男，教授，博士，研究方向：林木遗传育种及林业生物技术。E-mail: fanjf28@163.com
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