[1]马玉花,冶贵生,刘宝尧,等. 青海野生中国沙棘P5CS基因的扩增及序列分析[J].西北林学院学报,2014,29(05):88-91.[doi:doi:10.3969/j.issn.1001-7461.2014.05.16]
　MA Yu-hua,YE Gui-sheng,LIU Bao-yao,et al. Amplification and Sequence Analysis of P5CS in Wild Hippophae rhamnoides in Qinghai[J].JOURNAL OF NORTHWEST FORESTRY UNIVERSITY,2014,29(05):88-91.[doi:doi:10.3969/j.issn.1001-7461.2014.05.16]
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 青海野生中国沙棘P5CS基因的扩增及序列分析()
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《西北林学院学报》[ISSN:1001-7461/CN:61-1202/S]

卷:
第29卷
期数:
2014年05期
页码:
88-91
栏目:
出版日期:
2014-09-30

文章信息/Info

Title:
 Amplification and Sequence Analysis of P5CS in Wild Hippophae rhamnoides in Qinghai
文章编号:
1001-7461(2014)05-0088-04
作者:
 马玉花; 冶贵生; 刘宝尧; 张亚波; 郑娜
 （青海大学 农牧学院，青海 西宁 810016）
Author(s):
 MA Yu-hua;  YE Gui-sheng; LIU Bao-yao;  ZHANG Ya-bo;  ZHENG Na
 (College of Agriculture and Animal Husbandry, Qinghai University, Xining, Qinghai 810016, China)
关键词:
 ; 青海野生中国沙棘; P5CS基因; 扩增; 序列分析
Keywords:
Hippophae rhamnoides subsp. sinensis;  P5CS;  amplification;  sequence analysis
分类号:
S793.608
DOI:
doi:10.3969/j.issn.1001-7461.2014.05.16
文献标志码:
A
摘要:
 以青海野生中国沙棘的嫩叶为材料，扩增其P5CS基因并进行序列测定。结果表明：所得的中国沙棘的P5CS基因的碱基数量为434 bp，其中A碱基115个，T碱基119个， G碱基107个，C碱基93个。另外，通过对中国沙棘P5CS基因的氨基酸的序列分析显示，所得P5CS基因片段编码144个氨基酸，其中强碱性氨基酸21个，强酸性氨基酸8个，疏水性氨基酸53个，亲水性氨基酸34个。
Abstract:
 The fragment of P5CS of wild Hippophae rhamnoides subsp. sinensis in Qinghai province was amplified by the specific primers, then the nucleotide sequence and amino acid sequence of P5CS was identified. The sequence analysis indicated that the total length of P5CS was 434bp, and the nucleotide composition analysis showed that the number of A,T,G,C were 115, 119, 107 and 93, respectively. On the other hand, the total length of amino acid was 144, and the animo acid composition analysis deonstrated that there were 21 strongly basic (+) amino acids, 8 strongly acidic (-) amino acids, 53 hydrophobic amino acids and 34 polar amino acids.

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备注/Memo

备注/Memo:
 收稿日期：2013-12-12修回日期：2014-01-13
基金项目：中国科学院 “西部之光”人才培养计划项目。
作者简介：马玉花，女，回族，博士，副教授，研究方向：林木抗逆机理、野生植物资源开发利用。E-mail：sophere8@163.com
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