[1]王飞,樊金拴*,冯慧英,等. 青扦针叶总黄酮超声提取及抗氧化活性[J].西北林学院学报,2016,31(1):243-249.[doi:10.3969/j.issn.1001-7461.2016.01.42]
　WANG Fei,FAN Jin-shuan*,FENG Hui-ying,et al. Ultrasound Assisted Extraction and Antioxidant Activities of Total Flavonoids in the Needles of Picea wilsonii[J].JOURNAL OF NORTHWEST FORESTRY UNIVERSITY,2016,31(1):243-249.[doi:10.3969/j.issn.1001-7461.2016.01.42]
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 青扦针叶总黄酮超声提取及抗氧化活性()
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《西北林学院学报》[ISSN:1001-7461/CN:61-1202/S]

卷:
第31卷
期数:
2016年第1期
页码:
243-249
栏目:
出版日期:
2016-01-31

文章信息/Info

Title:
 Ultrasound Assisted Extraction and Antioxidant Activities of Total Flavonoids in the Needles of Picea wilsonii
文章编号:
1001-7461(2016)01-0243-07
作者:
 王飞; 樊金拴*; 冯慧英; 刘滨; 高智辉
 （西北农林科技大学 林学院，陕西 杨陵 712100）
Author(s):
 WANG Fei; FAN Jin-shuan*; FENG Hui-ying; LIU Bin; GAO Zhi-hui
 (Collge of Forestry,Northwest A&F University,Yangling,Shaanxi 712100,China)
关键词:
 ; 青扦针叶; 总黄酮; 超声提取; 响应面法
Keywords:
Picea wilsonii;  total flavonoid;  ultrasonic extraction;  response surface methodology
分类号:
S789.4
DOI:
10.3969/j.issn.1001-7461.2016.01.42
文献标志码:
A
摘要:
 对青扦针叶总黄酮超声提取工艺和抗氧化性进行了研究，在单因素试验基础上，采用响应面法优化青扦针叶总黄酮的提取工艺，并通过对DPPH·、ABTS·的清除试验以及与Vc、槲皮素和芦丁的总还原力进行比较，来研究青扦总黄酮的抗氧化活性。结果表明，在料液比为1∶20的条件下，得到黄酮的最佳提取工艺为：乙醇体积分数50.63%、超声功率319.63 W、超声时间49.46 min、超声温度为64.88℃，此时青扦黄酮的提取率可达4.118 69%。青扦针叶总黄酮对DPPH·清除率可达90.21%，清除ABTS·的Trolox当量为29 633.3 μmol·g-1，其还原能力优于芦丁，但不及Vc和槲皮素。表明青扦针叶总黄酮具有较强的抗氧化能力，可为青扦针叶黄酮资源的开发利用提供依据。
Abstract:
 The optimal ultrasound assisted extraction conditions of total flavonoids from Picea wilsonii needles were investigated,in which based on the single-factor tests,the optimal values were determined by response surface analysis.Antioxidant activity of the extract was measured.The results showed that with the ratio of material to solvent 1∶20,the optimum extraction conditions were ethanol concentration: 50.63%(V/V),power of the generator of the ultrasound of:319.63 W,time of ultrasound assisted extraction 49.46 min,temperature 64.88℃,the ultrasonic power 319.63 W,and ethanol volume fraction 50.63%,by which the extraction rate of total flavonoids was 4.118 69%.The scavenging rate of the extract on DPPH· was 90.21% and the equivalent ABTS scavenging rate was 29 633.3 μmol·g-1,and the reducing power was better than that of rutin,but not as good as Vc and quercetin,indicating that the antioxidant effects of the total flavonoids in the needles of P.wilsonii was good.The results could be provide references for the development and utilization of the needles of P.wilsonii.

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备注/Memo

备注/Memo:
 收稿日期：2015-03-19修回日期：2015-05-20
作者简介：王飞，女，硕士研究生，研究方向：植物资源利用。E-mail：milanfei2010@126.com
*通信作者：樊金拴，男，教授，研究方向：植物资源利用。E-mail：fanjinshuan@163.com
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