[1]张凯,尹子郡,张跃进,等. HPLC法测定复方乌骨藤胶囊中三七皂苷R1、人参皂苷Rg1和人参皂苷Rb1的含量[J].西北林学院学报,2016,31(1):250-253.[doi:10.3969/j.issn.1001-7461.2016.01.43]
　ZHANG Kai,YIN Zi-jun,ZHANG Yue-jin,et al. Measurement of the Contents of Notoginsenoside R1，Ginsenoside Rg1 and Ginsenoside Rb1 in Compound Wuguteng Capsules by HPLC[J].JOURNAL OF NORTHWEST FORESTRY UNIVERSITY,2016,31(1):250-253.[doi:10.3969/j.issn.1001-7461.2016.01.43]
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 HPLC法测定复方乌骨藤胶囊中三七皂苷R1、人参皂苷Rg1和人参皂苷Rb1的含量()
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《西北林学院学报》[ISSN:1001-7461/CN:61-1202/S]

卷:
第31卷
期数:
2016年第1期
页码:
250-253
栏目:
出版日期:
2016-01-31

文章信息/Info

Title:
 Measurement of the Contents of Notoginsenoside R1，Ginsenoside Rg1 and Ginsenoside Rb1 in Compound Wuguteng Capsules by HPLC
文章编号:
1001-7461(2016)01-0250-04
作者:
 张凯1; 2; 尹子郡2; 张跃进1; 梁宗锁1; 郭宏波1*
 （1.西北农林科技大学 生命科学学院，陕西省中药指纹图谱与天然产物库研究中心，陕西 杨陵 712100；2.杨凌无为制药集团有限公司，陕西 杨陵 712100）
Author(s):
 ZHANG Kai1; 2; YIN Zi-jun2; ZHANG Yue-jin1; LIANG Zong-suo1; GUO Hong-bo1*
 （1.College of Life Sciences,Northwest A&F University,Shaanxi Research Center of TCM Fingerprinting and NP Library,Yangling,Shaanxi 712100，China; 2.Wuwei Pharmaceutical Group Co.Ltd.,Yangling,Shaanxi 712100,China）
关键词:
 ; HPLC; 复方乌骨藤胶囊; 三七皂苷R1; 人参皂苷Rg1; 人参皂苷Rb1; 质量控制
Keywords:
 ; HPLC;  Compound Wuguteng Capsules;  notoginsenoside R1;  ginsenoside Rg1;  ginsenoside Rb1;  quality control
分类号:
S567.236
DOI:
10.3969/j.issn.1001-7461.2016.01.43
文献标志码:
A
摘要:
 建立HPLC法测定复方乌骨藤胶囊中三七皂苷R1、人参皂苷Rg1和人参皂苷Rb1的含量。采用Kromasil C18柱（4.6 mm×250 mm，5 μm）色谱柱，以乙腈（A）-水（B）为流动相进行梯度洗脱；流速1.0 mL·mL-1，柱温40℃。结果表明：三七皂苷R1在0.229～2.29 μg范围内线性关系良好（r=0.999 6），平均加样回收率（n=6）为98.7%；人参皂苷Rg1在0.814～8.14 μg范围内线性关系良好（r=1.000 0），平均加样回收率（n=6）为99.2%；人参皂苷Rb1在0.771～7.71 μg范围内线性关系良好（r=0.999 6），平均加样回收率（n=6）为99.3%。本方法操作简便，结果准确，灵敏度高，重现性好，可作为复方乌骨藤胶囊中所含三七的质量控制方法。
Abstract:
 To determine the contents of notoginsenoside R1,ginsenoside Rg1 and ginsenoside Rb1 in “Compound Wuguteng Capsules”.High performance liquid chromatography (HPLC) was adopted to conduct the measurement.The chromatographic conditions were as following:column:Kromasil C18(4.6 mm × 250 mm,5 μm),mobile phase; acetonitrile (A)-water (B) with gradient elution,flow rate:1.0 mL·min-1,column temperature:40℃.Results,The linear ranges of notoginsenoside R1,ginsenoside Rg1 and ginsenoside Rb1 were 0.229-2.29,0.814-8.14 and 0.771-7.71 μg with average recovery rates of 98.7%,99.2% and 99.3%(n=6),respectively.Conclusion：The method was simple and accurate with high reproducibility.It could be employed as a quality control method for the detection of Notoginseng radix et rhizoma in “Compound Wututeng Capsules”.

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备注/Memo

备注/Memo:
 收稿日期：2014-11-25修回日期：2015-05-15
基金项目：教育部高等学校博士学科点专项基金（20100204120027）；陕西省农业科技攻关项目（2011K01-18）。
作者简介：张凯，男，硕士，研究方向：药用植物遗传资源保护与可持续利用、功能基因。E-mail:36040406@qq.com
*通信作者：郭宏波，男，博士，副教授，硕士生导师，研究方向：植物遗传资源保护与可持续利用、功能基因。Email:guohongbo@163.com
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