[1]周正君,田晶,李周岐*. 花椒愈伤组织诱导及细胞悬浮培养研究[J].西北林学院学报,2016,31(4):153-156.[doi:doi:10.3969/j.issn.1001-7461.2016.04.26]
　ZHOU Zheng-jun,TIAN Jing,LI Zhou-qi*. Studies on the Callus Induction and Cell Suspension Culture of Zanthoxylum bungeanum[J].JOURNAL OF NORTHWEST FORESTRY UNIVERSITY,2016,31(4):153-156.[doi:doi:10.3969/j.issn.1001-7461.2016.04.26]
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 花椒愈伤组织诱导及细胞悬浮培养研究()
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《西北林学院学报》[ISSN:1001-7461/CN:61-1202/S]

卷:
第31卷
期数:
2016年第4期
页码:
153-156
栏目:
出版日期:
2016-07-31

文章信息/Info

Title:
 Studies on the Callus Induction and Cell Suspension Culture of Zanthoxylum bungeanum
文章编号:
1001-7461(2016)04-0153-04
作者:
 周正君; 田晶; 李周岐*
 （西北农林科技大学 西部环境与生态教育部重点实验室，陕西 杨陵 712100）
Author(s):
 ZHOU Zheng-jun; TIAN Jing; LI Zhou-qi*
 (Key Laboratory of Environment and Ecology in Western China,Ministry of Education ,Northwest A &F University,Yangling ,Shaanxi 712100 ,China)
关键词:
花椒; 愈伤组织; 继代培养; 细胞悬浮培养
Keywords:
Zanthoxylum bungeanum;  callus;  subculture;  cell suspension culture
分类号:
S573.9
DOI:
doi:10.3969/j.issn.1001-7461.2016.04.26
文献标志码:
A
摘要:
 以花椒试管苗叶片为试验材料，研究了不同激素浓度对花椒愈伤组织继代培养的影响，并以多次继代得到的疏松、活性高的愈伤组织建立花椒细胞悬浮系。结果表明：花椒愈伤组织最适继代培养基为MS+2,4-D 1.0 mg·L-1+6-BA 0.5 mg·L-1 +蔗糖 30 g·L-1 +琼脂 6 g·L-1；花椒细胞悬浮培养的最佳培养基和培养条件为：MS+2,4-D 1.0 mg·L-1+6-BA 1.0 mg·L-1+葡萄糖30 g·L-1，接种量0.05 g·mL-1，摇床转速110 r·min-1，25 ℃暗培养，该条件下悬浮细胞生长良好，最大生长量为4.52 g；花椒悬浮细胞生长呈“S”型曲线，最适继代周期为16 d。
Abstract:
 Plantlet leaves of Zanthoxylum bungeanum were taken as experimental materials for studying the effects of different concentrations of hormone on callus induction and subculture.The crisp callus was selected to establish cell suspension culture.The results showed that the best callus subculture medium was MS+2,4-D 1.0 mg·L-1+6-BA 0.5 mg·L-1+sucrose 30 g·L-1+agar 6 g·L-1.The best suspension culture medium for Z.bungeanum cells was MS+2,4-D 1.0 mg·L-1+6-BA 1.0 mg·L-1+glucose 30 g·L-1.The best culturing conditions were:inoculum size:0.05 g·mL-1,gyratory shaker rotation speed:110 r·min-1,temperature:25 ℃ in dark environment.Under these conditions suspended cells grew well and the maximum fresh weight of cells was 4.52 g.Suspended cells growth of Z.bungeanum exhibited “S-type” curve and optimal subculture cycle was 16 days.

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备注/Memo

备注/Memo:
 收稿日期：2015-09-19修回日期：2015-11-12
基金项目：国家林业局林业公益性行业科研专项（301204706）。
作者简介：周正君，男，在读硕士，研究方向：林木遗传育种。E-mail:863676841@qq.com
*通信作者：李周岐，男，教授，博士生导师，研究方向：林木遗传育种。E-mail:lizhouqi@nwsuaf.edu.cn
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