[1]邢小姣,陆婷,马楠,等. 垂盆草人工种子制作技术研究[J].西北林学院学报,2016,31(6):169-174.[doi:10.3969/j.issn.1001-7461.2016.06.29]
 XING Xiao-jiao,LU Ting,MA Nan,et al. Preparation Technique on Artificial Seeds of Sedum sarmentosum[J].JOURNAL OF NORTHWEST FORESTRY UNIVERSITY,2016,31(6):169-174.[doi:10.3969/j.issn.1001-7461.2016.06.29]
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 垂盆草人工种子制作技术研究()
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《西北林学院学报》[ISSN:1001-7461/CN:61-1202/S]

卷:
第31卷
期数:
2016年第6期
页码:
169-174
栏目:
出版日期:
2016-11-30

文章信息/Info

Title:
 Preparation Technique on Artificial Seeds of Sedum sarmentosum
文章编号:
1001-7461(2016)06-0169-06
作者:
 邢小姣1陆婷1马楠2雷江丽2*
 (1.新疆农业大学 林学与园艺学院,新疆 乌鲁木齐 830052;2.深圳市、中国科学院 仙湖植物园,广东 深圳 518000)
Author(s):
 XING Xiao-jiao1LU Ting1MA Nan2LEI Jiang-li2*
 (1.School of Forestry & Horticulture,Xinjiang Agricultural University,Urumqi ,Xinjiang 830052,China; 2.Fairy Lake Botanical Garden,Shenzhen & Chinese Academy of Sciences ,Shenzhen ,Guangdong 518004,China)
关键词:
 垂盆草人工种子人工胚乳
Keywords:
 Sedum sarmentosum artificial seed artificial endosperm
分类号:
S723.131
DOI:
10.3969/j.issn.1001-7461.2016.06.29
文献标志码:
A
摘要:
 选用垂盆草2~4 mm带腋芽茎段作为包埋外植体,研究海藻酸钠浓度、激素配比、活性炭、离子交换时间及人工种子培养条件对人工种子成型、萌发及成苗的影响,探索适合垂盆草的人工种子制作方法。结果表明,采用滴珠法,以MS+4%海藻酸钠+1.0 mg·L-1 6-BA+0.1 mg·L-1GA3+0.5 mg·L-1 NAA为人工胚乳,与2% CaCl2水溶液发生离子交换反应15 min后获得的人工种子呈规则的球形、富弹性,在MS培养基中培养20 d萌发率达95%,30 d生根率达90%,在培养皿中催芽成苗率达60%,为制备垂盆草人工种子的适宜方法。
Abstract:
 Effects of synthetic endosperm components,activated carbon and germination conditions on the germination and rooting rate of artificial seeds of Sedum sarmentosum were researched by using 2-4 mm shoot cuttings with the buds used as propagules.The optimal medium used as artificial endosperm was MS+4% sodium alginate+1.0 mg·L-1 6-BA+0.1 mg·L-1 GA3+0.5 mg·L-1 NAA.The spherical and elastic artificial seeds were prepared after dropping the shoot cuttings into the solution of 2% CaCl2 for l5 min.The germination rate of the artificial seeds was 95% after culture for 20 days in MS medium,the rooting rate was 90% after 30 days,and the seedling rate was 60% in a Petri dish germination.This formula was a suitable combination of artificial seeds for S.sarmentosum.

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备注/Memo

备注/Memo:
 收稿日期:2016-02-17修回日期:2016-05-24
基金项目:深圳市城市管理局基金项目(2015023)。
作者简介:邢小姣,女,在读硕士,研究方向:园林植物遗传育种。E-mail:xing-xiaojiao@qq.com
*通信作者:雷江丽,女,博士,教授级高工,研究方向:园林植物种质资源评价与利用。E-mail:lei-jl@163.com
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