[1]王军晓,李婷,马志博,等. 枣树MYB基因家族鉴定、果实表达和盐胁迫响应研究[J].西北林学院学报,2022,37(2):20-28.[doi:10.3969/j.issn.1001-7461.2022.02.03]
　WANG Jun-xiao,LI Ting,MA Zhi-bo,et al. Identification,Fruit Expression and Response to Salt Stress of MYB Gene Family in Ziziphus jujuba[J].JOURNAL OF NORTHWEST FORESTRY UNIVERSITY,2022,37(2):20-28.[doi:10.3969/j.issn.1001-7461.2022.02.03]
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 枣树MYB基因家族鉴定、果实表达和盐胁迫响应研究()
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《西北林学院学报》[ISSN:1001-7461/CN:61-1202/S]

卷:
第37卷
期数:
2022年第2期
页码:
20-28
栏目:
出版日期:
2022-03-31

文章信息/Info

Title:
 Identification,Fruit Expression and Response to Salt Stress of MYB Gene Family in Ziziphus jujuba
文章编号:
1001-7461(2022)02-0020-09
作者:
 王军晓; 李婷; 马志博; 黄建*
 （西北农林科技大学 林学院，国家林业和草原局黄土高原林木培育重点实验室，陕西 杨陵 712100）
Author(s):
 WANG Jun-xiao; LI Ting; MA Zhi-bo; HUANG Jian*
 (College of Forestry,Key Laboratory of Silviculture on the Loess Plateau,National Forestry and Grassland Administration/Northwest A&F University,Yangling 712100,Shaanxi,China)
关键词:
 ; 枣树; MYB转录因子; 果实发育成熟; 盐胁迫
Keywords:
Ziziphus jujuba;  MYB transcription factor;  fruit development and ripening;  salt stress
分类号:
S718.43
DOI:
10.3969/j.issn.1001-7461.2022.02.03
文献标志码:
A
摘要:
MYB是植物基因组中最大的转录因子基因家族之一，广泛参与到植物发育与形态建成、次生代谢物合成逆境胁迫等过程。基于骏枣基因组和果实发育与成熟的5个阶段（花后20、40、60、80、100 d）转录组数据，鉴定和分析ZjMYB转录因子家族成员，分析其在枣果实成熟过程中的表达规律，并进一步研究NaCl胁迫下酸枣幼苗ZjMYB基因的响应。结果表明，共筛选到210个ZjMYB转录因子，依据MYB保守域的特点将其分为3类：72个1R-MYB亚类，127个R2R3-MYB亚类、9个3R-MYB亚类和2个4R-MYB，分布于12条染色体上。在骏枣果实成熟过程中，分别有49、15、6、13、12个基因在花后20、40、60、80、100 d表达量最高。进化分析发现ZjMYB14、ZjMYB110与苹果、紫薯参与花青苷的合成基因MdMYB9、MdMYB11和IBMYB1关系较近。100 mmol/L NaCl胁迫下，ZjMYB基因在酸枣幼苗的根茎叶中具有明显的组织表达特异性。盐胁迫8 d时，MYB基因在叶、茎和根中分别有9、3、7个基因显著上调表达。进化分析发现ZjMYB176、ZjMYB112、ZjMYB2、ZjMYB205与盐胁迫调控有关的苹果MdSIMYB1、小麦TaMYB33关系较近。本研究结果为阐明MYB转录因子在果实成熟调控以及枣树适应盐胁迫中机制提供重要基础。
Abstract:
MYB is one of the largest transcription factor gene families in plant genome,which is widely involved in plant development and morphogenesis,secondary metabolite synthesis,and stress response.In this study,based on the genome of Ziziphus jujuba Junzao and the transcriptome data of 5 fruit development and maturity stages (20,40,60,80,100 d after flowering),the members of ZjMYB transcription factor family and their expression patterns during fruit ripening were identified and analyzed.In addition,responses of ZjMYB gene in Ziziphus acidojujuba seedlings under NaCl stress were further studied.The results showed that a total of 210 ZjMYB transcription factors were identified from the jujube genome,which could be divided into three subclasses according to feature of MYB conserved domains,i.e.,72 1R-MYB subclasses,127 R2R3-MYB subclasses,9 3R-MYB subclasses and 2 4R-MYB subclasses.Those genes were located on 12 chromosomes.During fruit ripening of Junzao,49,15,6,13,12 genes were upregulated at 20,40,60,80,100 DAF,and the number of MYB genes was the highest at the initial fruit enlargement stage (40 DAF).Evolutionary analysis showed that ZjMYB14 and ZjMYB110 were closely related to the genes MdMYB9,MdMYB11 and IBMYB1 genes involved in anthocyanin biosynthesis in apple and sweet potato.By analyzing the expression profile of MYBs exposure to salt stress,we found that ZjMYB genes expressed differentially among leaves,stem and roots.After 8 d of salt stress,9,3 and 7 MYB genes of were significantly up-regulated in leaves,stems and roots,respectively.Phylogenetic analysis showed that ZjMYB176,ZjMYB112,ZjMYB2,ZjMYB205 were closely related to the regulation of salt stress in apple MDSIMYB1 and wheat TAMYB33.Our study provided important MYB gene resources which were potential involved in regulating jujube fruit maturation and resistance to salt stress.

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备注/Memo

备注/Memo:
 收稿日期：2021-03-24修回日期：2021-05-12
基金项目：新疆生产建设兵团南疆重点产业创新发展支撑计划项目（Grant No.2017DB006-1)；国家自然基金项目（31870584）；西北农林科技大学“仲英青年学者” 基金项目。
第一作者：王军晓。研究方向：经济林抗逆。E-mail：18829029544@163.com
*通信作者：黄建，博士，副研究员，博士生导师。研究方向：经济林培育与育种。E-mail：huangj@nwsuaf.edu.cn
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