[1]刘亦菲,杨冰,周显臻,等. 落叶松-杨栅锈菌MlpNHA1-like基因的克隆及序列分析[J].西北林学院学报,2022,37(5):129-134.[doi:10.3969/j.issn.1001-7461.2022.05.18]
　LIU Yi-fei,YANG Bing,ZHOU Xian-zhen,et al. Cloning and Sequence Analysis of NHA1-like Gene from Melampsora larici-populina[J].JOURNAL OF NORTHWEST FORESTRY UNIVERSITY,2022,37(5):129-134.[doi:10.3969/j.issn.1001-7461.2022.05.18]
点击复制

 落叶松-杨栅锈菌MlpNHA1-like基因的克隆及序列分析()
分享到：

《西北林学院学报》[ISSN:1001-7461/CN:61-1202/S]

卷:
第37卷
期数:
2022年第5期
页码:
129-134
栏目:
出版日期:
2022-09-30

文章信息/Info

Title:
 Cloning and Sequence Analysis of NHA1-like Gene from Melampsora larici-populina
文章编号:
1001-7461(2022)05-0129-06
作者:
 刘亦菲; 杨冰; 周显臻; 李嘉雯; 于丹*
 (西北农林科技大学 林学院，陕西 杨陵 712100)
Author(s):
 LIU Yi-fei; YANG Bing; ZHOU Xian-zhen; LI Jia-wen; YU Dan*
 (College of Forestry,Northwest A&F University,Yangling 712100,Shaanxi,China)
关键词:
 ; 落叶松-杨栅锈菌; NHA1基因; 同源克隆; 序列分析
Keywords:
 ; Melampsora larici-populina;  NHA1 gene;  homology cloning;  sequence analysis
分类号:
S763.11
DOI:
10.3969/j.issn.1001-7461.2022.05.18
文献标志码:
A
摘要:
 落叶松-杨栅锈菌引起的杨树叶锈病是杨树的一种重要病害。通过系统发育分析和序列相似性比对确定落叶松-杨栅锈菌标准菌株基因ID 45128为酿酒酵母NHA1直系同源基因以及基因ID 116278为NHA1相关基因，分别命名为MlpNHA1和MlpNHA1-like。以夏孢子cDNA为模板，运用RT-PCR技术，同源克隆获得中国菌株MlpNHA1-like基因的ORF片段，即MlpNHA1-like (wh03)，长度为1 545 bp，编码514个氨基酸。结果表明，生物信息学预测分析显示MlpNHA1-like (wh03)蛋白具有与酿酒酵母NHA1蛋白相同的保守结构域c_cpa1，且同样具有系列疏水跨膜结构，亚细胞定位预测结果表明目的蛋白分布在质膜区域。通过克隆落叶松-杨栅锈菌中国菌株MlpNHA1-like基因并开展序列分析，为解析该锈菌MlpHOG1蛋白介导通路在病原菌侵染致病及响应外界胁迫中的作用提供帮助。
Abstract:
 The poplar leaf rust caused by Melampsora larici-populina (Mlp) is an important disease of poplar.Phylogenetic and BLAST analyses revealed that ID 45128 was the lineal homogeneous gene of NHA1 from Saccharomyces cerevisiae,and ID 116278 was the relative gene of NHA1,which were named as MlpNHA1 and MlpNHA1-like,respectively.The gene MlpNHA1-like (wh03) was cloned from the urediospore cDNA of Mlp Chinese strain using the homology-based RT-PCR approach,with a 1 545 bp ORF encoding 514 amino acid residues.Protein sequence analysis showed that the deduced MlpNHA1-like (wh03) protein has a conserved c_cpa1 domain and several hydrophobic transmembrane domains as well as the NHA1 protein.The subcellular localization prediction indicated that the deduced MlpNHA1-like (wh03) protein was localized at the plasma membrane.This study is helpful to understand the roles of MlpHOG1-mediated signaling pathway during pathogenesis and responses to environmental stresses in M.larici-populina.

相似文献/References:

[1]刘春梅,曹支敏,余仲东.中国落叶松-杨栅锈菌生理小种鉴定[J].西北林学院学报,2008,23(03):105.
　LIU Chunmei,CAO Zhimin,YU Zhongdong.Physiological Race Indentification of Chinese Melampsora lariciˉpopul ina[J].JOURNAL OF NORTHWEST FORESTRY UNIVERSITY,2008,23(5):105.
[2]刘春梅,曹支敏,余仲东.中国落叶松-杨栅锈菌生理小种鉴定[J].西北林学院学报,2008,23(02):105.
　LIU Chunmei,CAO Zhimin,YU Zhongdong.Physiological Race Indentification of Chinese Melampsora lariciˉpopul ina[J].JOURNAL OF NORTHWEST FORESTRY UNIVERSITY,2008,23(5):105.
[3]田呈明,李振岐,康振生.青杨叶锈病(Melampsora larici-populina Kleb.)研究进展[J].西北林学院学报,1999,14(03):0.
　Tian Chengming,Li Zhenqi,Kang Zhensheng.Advances in Researches of Cathay Poplar Leaf Rust(Melampsora larici-populina Kleb.)[J].JOURNAL OF NORTHWEST FORESTRY UNIVERSITY,1999,14(5):0.
[4]任本权,曹支敏,潘彦平,等.落叶松-杨栅锈菌致病性分化研究[J].西北林学院学报,2003,18(02):51.
　Pathogenic Specialization in Melampsora larici-populina[J].JOURNAL OF NORTHWEST FORESTRY UNIVERSITY,2003,18(5):51.
[5]田爱霞,吴春芳,梁英梅.落叶松-杨栅锈菌重寄生菌的生物学特性研究[J].西北林学院学报,2005,20(04):108.
　TIAN Ai-xia,WU Chun-fang,LIANG Ying-mei.The Biological Characteristics of a Hyperparasite Melampsora larici-populina[J].JOURNAL OF NORTHWEST FORESTRY UNIVERSITY,2005,20(5):108.
[6]李亚藏,梁彦兰,王庆成. 铅对山梨和山荆子光合作用和叶绿素荧光特性的影响[J].西北林学院学报,2012,27(05):21.
　LI Ya-cang,LIANG Yan-lan,WANG Qing-cheng. Influence of Pb on Photosynthesis and Chlorophyll Fluorescence Characteristics in Pyrus ussuriensis and Malus baccata [J].JOURNAL OF NORTHWEST FORESTRY UNIVERSITY,2012,27(5):21.
[7]张小咏,李佳,杨艳昭,等. 基于SWAT模型的长江源区径流模拟[J].西北林学院学报,2012,27(05):38.
　ZHANG Xiao-yong,LI Jia,YANG Yan-zhao,et al. Runoff Simulation of the Catchment of the Headwaters of the Yangtze River Based on SWAT Model[J].JOURNAL OF NORTHWEST FORESTRY UNIVERSITY,2012,27(5):38.
[8]杨红旗,陈广辉,王金林. PVAc改性酚醛树脂制备铝木复合材料研究[J].西北林学院学报,2013,28(01):170.[doi:10.3969/j.issn.1001-7461.2013.01.34]
　YANG Hong-qi,CHEN Guang-hui,WANG Jin-lin. PF Adhesive Modified by PVAc for Manufacturing Technology of Decorative Fiberboard with Aluminum Foils[J].JOURNAL OF NORTHWEST FORESTRY UNIVERSITY,2013,28(5):170.[doi:10.3969/j.issn.1001-7461.2013.01.34]
[9]江涛,李秀荣,谢延军,等. 微波预处理对巨尾桉木材渗透性的影响[J].西北林学院学报,2013,28(01):174.[doi:10.3969/j.issn.1001-7461.2013.01.35]
　JIANG Tao,LI Xiu-rong,XIE Yan-jun,et al. Effects of Microwave Pretreatment on the Permeability of Eucalyptus grandis×Eucalyptus urophylla[J].JOURNAL OF NORTHWEST FORESTRY UNIVERSITY,2013,28(5):174.[doi:10.3969/j.issn.1001-7461.2013.01.35]
[10]牛晓霆,王逢瑚,曹新民. 明清家具匠师原木下料的工艺原则及措施[J].西北林学院学报,2013,28(01):178.[doi:10.3969/j.issn.1001-7461.2013.01.36]
　NIU Xiao-ting,WANG Feng-hu,CAO Xin-min. Techniques and Principles of Log Cuttings of Furniture Makers in Ming and Qing Dynasties[J].JOURNAL OF NORTHWEST FORESTRY UNIVERSITY,2013,28(5):178.[doi:10.3969/j.issn.1001-7461.2013.01.36]
[11]费昭雪,陈祖静,曹支敏,等. 叶锈菌对不同杨树品种叶片木质素的影响[J].西北林学院学报,2012,27(01):124.
　FEI Zhao-xue,CHEN Zu-jing,CAO Zhi-min,et al. Effects of Melampsora larici-populina Kleb. on Leaf Lignin of Poplar Varieties with Different Resistance[J].JOURNAL OF NORTHWEST FORESTRY UNIVERSITY,2012,27(5):124.
[12]陈祖静,费昭雪,曹支敏,等. 杨树与叶锈菌互作中的细胞程序性死亡[J].西北林学院学报,2013,28(03):134.[doi:10.3969/j.issn.1001-7461.2013.03.26]
　CHEN Zu-jing,FEI Zhao-xue,CAO Zhi-min,et al. Programmed Cell Death in the Interaction between Poplar and Leaf Rust[J].JOURNAL OF NORTHWEST FORESTRY UNIVERSITY,2013,28(5):134.[doi:10.3969/j.issn.1001-7461.2013.03.26]
[13]杨冰,陈凯玥,李子晔,等. 落叶松-杨栅锈菌MlpMCM4蛋白和MlpHOG1蛋白互作关系初探[J].西北林学院学报,2024,39(1):100.[doi:10.3969/j.issn.1001-7461.2024.01.14]
　YANG Bing,CHEN Kai-yue,LI Zi-ye,et al. A Preliminary Study on the Interaction Between MlpMCM4 and MlpHOG1 in Melampsora larici-populina[J].JOURNAL OF NORTHWEST FORESTRY UNIVERSITY,2024,39(5):100.[doi:10.3969/j.issn.1001-7461.2024.01.14]

备注/Memo

备注/Memo:
 收稿日期：2021-08-21修回日期：2021-09-09
基金项目：国家自然科学基金（31870630）。
第一作者：刘亦菲。研究方向：林木病害病原菌致病机理。E-mail：ayezoe9709@126.com
*通信作者：于丹，博士，副教授。研究方向：林木病害病原菌致病机理。E-mail：yudan@nwsuaf.edu.cn
更新日期/Last Update: