[1]周松坤,宋西德*,张宗勤,等.良种沙棘“实优1号”组织培养研究[J].西北林学院学报,2006,21(03):67.
 ZHOU Songkun,SONG Xide,ZHANG Zongqin,et al.A Study on Tissue Culture of Fine Variety “Shiyou 1” of Hippophae rhamnoides[J].JOURNAL OF NORTHWEST FORESTRY UNIVERSITY,2006,21(03):67.
点击复制

良种沙棘“实优1号”组织培养研究()
分享到:

《西北林学院学报》[ISSN:1001-7461/CN:61-1202/S]

卷:
第21卷
期数:
2006年03期
页码:
67
栏目:
林木培育
出版日期:
2006-06-30

文章信息/Info

Title:
A Study on Tissue Culture of Fine Variety “Shiyou 1” of Hippophae rhamnoides
作者:
周松坤1宋西德1*张宗勤1翟巧绒2张永1周锋利1
1. 西北农林科技大学 林学院,陕西 杨陵 712100; 2. 陕西省林业勘察设计院,陕西 西安 710082
Author(s):
ZHOU Songkun1SONG Xide1 ZHANG Zongqin1ZHAI Qiaorong2ZHANG Yong1ZHOU Fengli1
1. College of Forestry, Northwest A & F University , Yangling, Shaanxi 712100, China; 2. Forestry Institute of Surveying and Design of Shaanxi Province, Xian, Shaanxi 710082, China
关键词:
沙棘水培组织培养不定芽
Keywords:
Hippophae rhamnoides hydroponics tissue culture adventitious bud
分类号:
S793.6;S722.37
文献标志码:
A
摘要:
以良种沙棘“实优1号”的水培茎尖和叶片为外植体进行了不定芽及愈伤组织诱导试验。结果表明,水培茎尖在1/2 MS + 6BA 1.0 mg·L-1+ IAA 0.5 mg·L-1上可诱导出大量愈伤组织及不定芽,继代转接可获得丛生芽。幼苗茎段在1/2 M S + 6BA 2.5 mg·L-1 + IAA 0.2 mg· L-1上可诱导腋芽增殖,但倍数较低;水培叶在1/2 MS + 6BA 0.5 mg·L-1 + KT 0.2 mg·L-1 + NAA 0.02 mg·L-1上诱导效果好,不定芽多,分化速度快, 30 d平均分化不定芽9.1个,分化率达94.1%。继代苗在1/2 B5+6BA 0.3 mg·L-1 + IBA 0.4 mg·L-1上有较高的生根率,达到87.5%。西北林学院学报21卷第3期周松坤等良种沙棘“实优1号”组织培养研究
Abstract:
The hydroponics stem apexes and leaves of “Shiyou 1”(Hippophae rhamnoides ) were used as explants to induce adventitious buds and callus. The results showed that adventitious buds and callus could be obtained from stem apexes on 1/2 MS + 6BA 1.0 mg·L-1 + IAA 0.5 mg·L-1, and cluster shoots were got by subculture. Axillary buds could be induced on 1/2 MS+ 6BA 2.5 mg· L-1+ IAA 0.2 mg·L-1 from juvenile stems, but the propagation rate was low. On 1/2 MS + 6BA 0.5 mg·L-1 + KT 0.2 mg·L-1+ NAA 0.02 mg·L-1, an average of 9.1 adventitious buds per explant were induced effectively and easily from hydroponics leaves after thirty days, the differentiation rate was up to 94.1%. On 1/2 B5 + 6BA 0.3 mg·L-1 + IBA 0.4 mg·L-1, roots were induced with a high inducing rate of 87.5% on the subcultured plantlets.

参考文献/References:

[1]王俊峰, 梁宗锁. 沙棘生物学特性与利用[M]. 西安 陕西科学技术出版社, 1999.
[2]徐虹, 王俊峰, 梁宗锁. 沙棘组织培养技术研究现状及存在问题[J]. 沙棘, 1999,12 (1) 1113.
[3]李师翁, 范小峰, 卢东平. 大果良种沙棘愈伤组织诱导及植株再生的研究[J]. 西北植物学报, 2001,21 (2) 262266.
[4]孙兰英, 单金友, 王春艳,等. 沙棘组织培养培养基筛选试验[J]. 沙棘, 1998, 11 (3) 1415.
[5]康冰, 张广军, 吕月玲,等. 俄罗斯大果沙棘组织培养技术研究[J]. 西北农林科技大学学报(自然科学版), 2002,30 (6) 162165.
[6]赵国林,刘金郎,朱滨. 沙棘的组织培养和植株再生[J]. 植物生理学通讯,1989,25 (1) 42.
[7]郭春华, 徐玉霞. 沙棘优良品系茎尖组织培养技术初探[J]. 沙棘, 2000,13 (1) 2627.
[8]吕月玲, 张广军, 康冰. 俄罗斯大果沙棘离体快繁研究[J]. 湖南农业大学学报(自然科学版), 2002,28 (5) 405407.
[9]杨丽萍, 张虎林, 赵秀梅. 沙棘离体快速繁育技术研究[J]. 国际沙棘研究与开发, 2004, 2 (1) 1216.
[10]徐虹,梁宗锁.沙棘组织培养技术研究[J]. 西北植物学报,2001,21 (2) 267272.

相似文献/References:

[1]车少辉,唐德瑞*,潘 静,等.油松针叶束水培生根的研究[J].西北林学院学报,2008,23(03):110.
 CHE Shaohui,TAN G Derui,PAN Ji ng,et al.Rooting of Needle Fascicles of Pinust abulae formis by Water Culture[J].JOURNAL OF NORTHWEST FORESTRY UNIVERSITY,2008,23(03):110.
[2]吴敏,宋西德*,周锋利,等.柴松针叶束水培生根的研究柴松针叶束水培生根的研究[J].西北林学院学报,2009,24(02):60.
 WU Min,SONG Xi de*,ZHOU Feng li,et al.Rooting of Needle Fascicles of Pinus tabulaeformis f. shekannesis by Water Culture[J].JOURNAL OF NORTHWEST FORESTRY UNIVERSITY,2009,24(03):60.
[3]侯利涵,黄孝风,陈慧晶,等. 水培条件下毛竹幼苗的氮响应研究[J].西北林学院学报,2019,34(6):49.[doi:10.3969/j.issn.1001-7461.2019.06.08]
 HOU Li-han,HUANG Xiao-feng,CHEN Hui-jing,et al. Growth Response of Phyllostachys edulis Seedlings to Different Nitrogen Concentrations under Hydroponic Conditions[J].JOURNAL OF NORTHWEST FORESTRY UNIVERSITY,2019,34(03):49.[doi:10.3969/j.issn.1001-7461.2019.06.08]

备注/Memo

备注/Memo:
收稿日期 20050906修回日期 20051103基金项目 国家林业局“黄土高原抗逆性良种推广”项目(2001天保02号)作者简介 周松坤(1979),男,河南南阳人,在读硕士,主要从事林木育苗研究。*通讯作者 宋西德,男,研究员,研究方向为林木种苗繁育理论与技术。
更新日期/Last Update: 2010-05-04