[1]陈艺,郭军战*,李辉,等.15个果桑无性系指纹图谱的构建[J].西北林学院学报,2011,26(06):62-65.
　CHEN Yi,GUO Jun zhan,LI Hui,et al.Fingerprints Construction of 15 Fruit Mulberry (Morus spp.) Clones[J].JOURNAL OF NORTHWEST FORESTRY UNIVERSITY,2011,26(06):62-65.
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15个果桑无性系指纹图谱的构建
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《西北林学院学报》[ISSN:1001-7461/CN:61-1202/S]

卷:
第26卷
期数:
2011年06期
页码:
62-65
栏目:
林木遗传育种与栽培
出版日期:
2011-11-30

文章信息/Info

Title:
Fingerprints Construction of 15 Fruit Mulberry (Morus spp.) Clones
作者:
陈艺1; 郭军战1*; 李辉2; 姚忙珍3
1.西北农林科技大学 林学院，陕西 杨陵 712100；2.户县林业局，陕西 户县 710300; 3. 渭南市林业技术推广站，陕西 渭南 714000
Author(s):
CHEN Yi1;  GUO Junzhan1; LI Hui2;  YAO Mangzhen3
1.College of Forestry, Northwes A & F University, Yangling, Shaanxi 712100, China; 2.Huxia Forest Bureau, Huxian, Shaanxi 710300, China; 3.Forestry Extension Station, Weinan, Shaanxi 714000, China
关键词:
果桑; 无性系; 指纹图谱; RAPD
Keywords:
fruit mulberry;  clones;  fingerprint;  RAPD
分类号:
S792.990.4
文献标志码:
A
摘要:
用改良的CTAB法提取15份果桑无性系的基因组DNA，通过RAPD分子标记技术，构建不同果桑无性系的指纹图谱。结果表明：每条引物扩增出8~15条清晰地条带，其中多态性条带数为4~15条，多态性比例为50.0%~100.0%。20条随机引物共得到232条清晰的条带，多态性条带为178条，平均多态性比例为76.7%；利用随机引物OPA09和OPD05的扩增产物电泳图为基础构建的指纹图谱可以鉴定出15个果桑无性系。
Abstract:
Modified CTAB method was adopted to extract total DNA of 15 fruit mulberry (Morus spp.) clones. The RAPD fingerprints of 15 fruit mulberry clones were constructed. The results showed that 8-15 bands were clearly amplified from each primers, of which, 4-15 bands were of polymorphism and the percentages of primers with polymorphism were from 50.0% to 100.0%. Totally 232 bands were produced using 20 random primers, in which 178 bands showed polymorphism. Fifteen fruit mulberry clones can be identified by using the fingerprints established on the electrophoresis map of products amplified by primers of OPA09 and OPD05.

参考文献/References:

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备注/Memo

备注/Memo:
收稿日期：20101009修回日期：20101229 基金项目：西北农林科技大学植物遗传育种专项（A212020503） 作者简介：陈艺，女，在读硕士研究生，主要从事林业分子生物技术研究。 *通讯作者：郭军战，男，副教授，主要从事林业遗传育种研究。
更新日期/Last Update: 2011-12-31