[1]罗清,卢业飞,於艳萍,等. 24份杜鹃属植物的ISSR分子鉴定[J].西北林学院学报,2016,31(3):154-158.[doi:10.3969/j.issn.1001-7461.2016.03.26]
　LUO Qing,LU Ye-fei,YU Yan-ping,et al. Identification of 24 Rhododendron Plants by ISSR Molecular Markers[J].JOURNAL OF NORTHWEST FORESTRY UNIVERSITY,2016,31(3):154-158.[doi:10.3969/j.issn.1001-7461.2016.03.26]
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 24份杜鹃属植物的ISSR分子鉴定()
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《西北林学院学报》[ISSN:1001-7461/CN:61-1202/S]

卷:
第31卷
期数:
2016年第3期
页码:
154-158
栏目:
出版日期:
2016-05-31

文章信息/Info

Title:
 Identification of 24 Rhododendron Plants by ISSR Molecular Markers
文章编号:
1001-7461(2016)03-0154-05
作者:
 罗清; 卢业飞; 於艳萍; 毛立彦; 严霖; 池昭锦*
 （广西壮族自治区 亚热带作物研究所，广西 南宁 530001）
Author(s):
 LUO Qing; LU Ye-fei; YU Yan-ping; MAO Li-yan; YAN Lin; CHI Zhao-jin*
 (Guangxi Subtropical Crops Research Institute,Nanning,Guangxi 530001,China)
关键词:
 ; ISSR; 杜鹃属; 亲缘关系; 鉴定
Keywords:
 ; ISSR;  Rhododendron;  genetic relationship;  identification
分类号:
S722.33
DOI:
10.3969/j.issn.1001-7461.2016.03.26
文献标志码:
A
摘要:
 采用ISSR分子标记对24份杜鹃属植物进行亲缘关系分析， 7个ISSR引物共扩增出92条谱带，其中89条为多态性谱带，多态性比率达96.7%。利用NTSYS-pc2.10e软件计算24份杜鹃属植物间的遗传相似系数，变化范围在0.456 5~0.782 6之间，平均相似系数为0.613 4。通过UPGMA聚类分析发现，24份杜鹃属植物被划分为3大类，聚类结果与地理分布存在一定的相关性，其中第2类与形态学分类基本吻合，表明24份杜鹃属植物的ISSR扩增图谱差异较大，易于区分，可用于杜鹃属植物的鉴定。
Abstract:
 ISSR molecular marker was used to study the genetic relationships of 24 samples of Rhododendron plants,a total of 92 bands were amplified by 7 ISSR primers,89 of which were polymorphic ones,accounting for 96.7% of the total.The genetic similarity coefficients varied from 0.456 5 to 0.782 6 with an average of 0.613 4,computed with software NTSYSpc2.10e.According to the UPGMA method,24 Rhododendron samples could be divided into three groups,clustering result with geographical distribution had certain relevance,and the second group was in accordance with the basic morphological classification.The result confirmed that 24 Rhododendron plants were easily identified owning to distinct diverse ISSR polymorphic bands,so this may be used for the identification of the Rhododendron plants.

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备注/Memo

备注/Memo:
 收稿日期：2015-11-29修回日期：2016-01-17
基金项目：广西壮族自治区直属公益性科研院所基本科研业务费专项(桂热研201412)。
作者简介：罗清，女，硕士，助理研究员，研究方向：植物种质资源收集、育种与栽培。E-mail:lqlq522@163.com
*通信作者：池昭锦，男，工程师，研究方向：热带作物栽培。E-mail:973919489@qq.com
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