[1]张梅,潘大仁. HPLC同时测定锦绣杜鹃四种黄酮类成分的含量[J].西北林学院学报,2013,28(01):119-122.[doi:10.3969/j.issn.1001-7461.2013.01.23]
　ZHANG Mei,PAN Da-ren. Simultaneous Determination of Four Flavonoids in the Leaves of Rhododendron pulchrum by HPLC[J].JOURNAL OF NORTHWEST FORESTRY UNIVERSITY,2013,28(01):119-122.[doi:10.3969/j.issn.1001-7461.2013.01.23]
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 HPLC同时测定锦绣杜鹃四种黄酮类成分的含量()
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《西北林学院学报》[ISSN:1001-7461/CN:61-1202/S]

卷:
第28卷
期数:
2013年01期
页码:
119-122
栏目:
出版日期:
2013-01-30

文章信息/Info

Title:
 Simultaneous Determination of Four Flavonoids in the Leaves of Rhododendron pulchrum by HPLC
文章编号:
1001-7461(2013)01-0119-04
作者:
 张梅;  潘大仁
 (1.闽江学院 化工系，福建 福州 350108；2.福建农林大学 生命科学学院，福建 福州 350002)
Author(s):
 ZHANG Mei; PAN Da-ren
 (1.Department of Chemistry and Chemical Engineering,Minjiang University,Fuzhou,Fujian 350108,China;2.College of Life Science,Fujian Agricultural and Forestry Universities,Fuzhou,Fujian 350002,China)
关键词:
 ; HPLC;  锦绣杜鹃;  黄酮类化合物;  含量测定
Keywords:
 ; HPLC; Rhododendron pulchrum; flavonoids; content determination
分类号:
Q946.83
DOI:
10.3969/j.issn.1001-7461.2013.01.23
文献标志码:
A
摘要:
 在建立高效液相色谱法（HPLC）同时，测定锦绣杜鹃中金丝桃苷、异槲皮苷、槲皮苷、槲皮素4种黄酮类化合物的含量。采用Waters C18 (4.6 mm×250 mm，5 μm) 反相色谱柱，甲醇(B)和水(A)为流动相，梯度洗脱程序为：0~24 min，31%~42% B；24~30 min，42%~50% B；30~35 min，50%~60% B；35~40 min，60% B；检测波长356 nm，流速为0.7 mL·min-1，柱温30 ℃。结果显示锦绣杜鹃中4种黄酮类化合物达到了较好的分离，其中平均回收率分别为97.73%、101.71%、98.70%、104.28%；RSD分别为2.25%、1.31%、1.82%、2.81%(n=5)，说明该分析方法简便、准确、重现性好,适合于锦绣杜鹃中黄酮类化合物含量的测定，其中锦绣杜鹃中黄酮类成分在4月份达到最大值。
Abstract:
 The objective of this study was to establish a HPLC method for simultaneous determination of 4 kinds of flavonoids (quercetin-3-galactoside,quercetin-3-glucoside,quercitrin,and quercetin ) in the leaves of Rhododendron pulchrum.The following system was found to be optimum for the analysis:Waters C18 column ( 4.6 mm×250 mm，5 μm) by gradient elution with mobile phase A (water) and B (methanol):0-24 min,31%-42% B;24-30 min,42%-50% B;30-35 min,50%-60% B;35-40 min,60% B.The detection wavelength was 356 nm,and the rate flow was 0.7 mL/min with column temperature at 30℃.Under these conditions,4 types of flavonoids could be well separated.The average recovery of 4 types of flavoniods (quercetin-3-galactoside,quercetin-3-glucoside,quercitrin,and quercetin ) were 97.73%,101.71%,98.70% and 104.28%,and the relative standard deviations of the method were 2.25%,1.31%,1.82% and 2.81% (n=5),respectively.The method was accurate and sensitive，which could be used for the determination of flavoniods in the leaves of R.pulchrum.The contents of flavonoids were in the highest levels in april.

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备注/Memo

备注/Memo:
 收稿日期：2012-04-07 修回日期：2012-05-09
基金项目：福建省教育厅重点学科项目（OB08B005）；福建省教育厅科技项目（JB12164）。
作者简介：张梅，女，博士，讲师，主要研究方向：植物生物化学。E-mail:zhangmei11@163.com
*通信作者：潘大仁，男，博士，教授，博士生导师，主要研究方向：生物技术。E-mail:pandaren@yahoo.com.cn
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