[1]魏军坤,刘闵豪,杨秀平*,等. HPLC同时测定杜仲叶中绿原酸和芦丁方法的建立及在杂种后代选择中的应用[J].西北林学院学报,2014,29(05):129-132.[doi:doi:10.3969/j.issn.1001-7461.2014.05.24]
　WEI Jun-kun,LIU Min-hao,YANG Xiu-ping*,et al.Simultaneous Determination of Chlonogenic Acid and Rutin in the Leaves of Eucommia ulmoides by HPLC and Its Use in the Selection of Hybrids[J].JOURNAL OF NORTHWEST FORESTRY UNIVERSITY,2014,29(05):129-132.[doi:doi:10.3969/j.issn.1001-7461.2014.05.24]
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 HPLC同时测定杜仲叶中绿原酸和芦丁方法的建立及在杂种后代选择中的应用()
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《西北林学院学报》[ISSN:1001-7461/CN:61-1202/S]

卷:
第29卷
期数:
2014年05期
页码:
129-132
栏目:
出版日期:
2014-09-30

文章信息/Info

Title:
Simultaneous Determination of Chlonogenic Acid and Rutin in the Leaves of Eucommia ulmoides by HPLC and Its Use in the Selection of Hybrids
文章编号:
1001-7461(2014)05-0129-04
作者:
 魏军坤; 刘闵豪; 杨秀平*; 李煜
 （西北农林科技大学 林学院，陕西 杨陵 712100）
Author(s):
 WEI Jun-kun;  LIU Min-hao;  YANG Xiu-ping*;  LI Yu
 (College of Forestry, Northwest A&F University, Yangling, Shaanxi 712100, China)
关键词:
 ; 杜仲; HPLC; 绿原酸; 芦丁; 优良单株
Keywords:
Eucommia ulmoides;  HPLC;  chlonogenic;  rutin;  superior individual
分类号:
S781.41
DOI:
doi:10.3969/j.issn.1001-7461.2014.05.24
文献标志码:
A
摘要:
 建立利用高效液相法（HPLC）同时测定杜仲叶中绿原酸和芦丁的方法。色谱柱为Aglient ZORBAX SB-C18（4.6 mm×250 mm，5 μm），流动相组成为甲醇-乙酸-水（体积比为50∶1∶49），检验波长为340 nm，流量为1 mL/min，检测温度为32℃。在该色谱条件下，绿原酸和芦丁分别在0.060 0～2.000 0 μg/mL和0.001 8～0.090 0 μg/mL范围内线性关系良好，相关系数分别为0.999 7和0.999 3。利用此方法对杜仲杂交后代中41株生长性状表现优良的个体进行了叶片中绿原酸和芦丁含量的测定，为杜仲优良无性系的选择提供依据。
Abstract:
 A convenient high performance liquid chromatographic (HPLC) method has been established for the simultaneous determination of chlonogenic acid and rutin in the leaves of Eucommia ulmoides. Two components were saperated by the column Aglient ZORBAX SB-C18 (4.6 mm×250 mm, 5 μm) with a mobile phase of methanol-acetic acid-water (50∶1∶49, V/V) at a flow rate of 1 mL/min, detection wavelength of 240 nm and column temperature of 32℃. The chlonogenic and rutine each showed good liner relationship in a range of 0.060 0 μg/mL～2.000 0 μg/mL and 0.001 8 μg/mL～0.090 0 μg/mL, respectively, with the correlation cofficients of 0.999 7 and 0.999 3. The two components in the leaves of 41 fine individual clones were determined using the method above to provide basis for selection of superior clones.

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备注/Memo

备注/Memo:
 收稿日期：2013-12-16修回日期：2014-01-13
基金项目：林业公益性行业科研专项（201204605）。
作者简介：魏军坤，男，在读硕士，研究方向：林木遗传育种。E-mail：jun_kun@126.com
*通信作者：杨秀平，女，高级实验师，研究方向：林产化工。E-mail:302671019@qq.com
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