[1]蔡三山,欧阳静,王义勋,等. 10株白僵菌菌株ITS序列测定和系统发育分析[J].西北林学院学报,2016,31(5):182-187.[doi:10.3969/j.issn.1001-7461.2016.05.30]
 CAI San-shan,OUYANG Jing,WANG Yi-xun,et al. Internal Transcribed Spacer Region Sequencing and Phylogenetic Analysis of 10 Strains of Beauveria spp.[J].JOURNAL OF NORTHWEST FORESTRY UNIVERSITY,2016,31(5):182-187.[doi:10.3969/j.issn.1001-7461.2016.05.30]
点击复制

 10株白僵菌菌株ITS序列测定和系统发育分析()
分享到:

《西北林学院学报》[ISSN:1001-7461/CN:61-1202/S]

卷:
第31卷
期数:
2016年第5期
页码:
182-187
栏目:
出版日期:
2016-09-30

文章信息/Info

Title:
 Internal Transcribed Spacer Region Sequencing and Phylogenetic Analysis of 10 Strains of Beauveria spp.
文章编号:
1001-7461(2016)05-0182-06
作者:
 蔡三山1欧阳静2王义勋1查玉平1洪承昊1陈京元1*
 (1.湖北省林业科学研究院,湖北 武汉 430075;2.湖北省公安县植物保护局,湖北 公安 434300)
Author(s):
 CAI San-shan1OUYANG Jing2WANG Yi-xun1ZHA Yu-ping1HONG Cheng-hao1CHEN Jing-yuan1*
 (1.Hubei Academy of Forestry,Wuhan,Hubei 430075,China; 2.Bureau of Plant Protection,Gongan County,Gongan,Hubei 434300,China)
关键词:
 白僵菌ITS序列系统发育
Keywords:
Beauveria spp. ITS Region phylogenetic analysis
分类号:
S763.15
DOI:
10.3969/j.issn.1001-7461.2016.05.30
文献标志码:
A
摘要:
 通过对10株白僵菌菌株进行培养和提取基因组DNA,并利用真菌ITS序列通用引物ITS4和ITS5进行PCR扩增,均得到了1条长约500 bp DNA产物。分别对该产物进行序列测定和分析表明,这10株白僵菌菌株的ITS序列长为490~494 bp;利用ITS序列构建系统发育树表明,菌株YD-1、YD-2、JN-1、QC-1、SY-1、YC-1、YC-2、GA-1和FJ-2均为球孢白僵菌(Beauveria bassania),而菌株FJ-1很可能为白僵菌属的一个新成员。
Abstract:
 Based on cultivation and extracting genomic DNA of 10 strains of Beauveria spp.,we amplified a DNA fragment with a length of about 500 bp and the fungal universal primers,ITS4 and ITS5.Sequence analysis showed that the length of ITS region of 10 strains was from 490 bp to 494 bp.Phylogenetic analysis demonstrated that the 9 strains,including YD-1,YD-2,JN-1,QC-1,SY-1,YC-1,YC-2,GA-1,and FJ-2 belonged to Beauveria bassania and the left strain FJ-1 maybe a new member of genus Beauveria.

相似文献/References:

[1]李亚藏,梁彦兰,王庆成. 铅对山梨和山荆子光合作用和叶绿素荧光特性的影响[J].西北林学院学报,2012,27(05):21.
 LI Ya-cang,LIANG Yan-lan,WANG Qing-cheng. Influence of Pb on Photosynthesis and Chlorophyll Fluorescence Characteristics in Pyrus ussuriensis and Malus baccata [J].JOURNAL OF NORTHWEST FORESTRY UNIVERSITY,2012,27(5):21.
[2]张小咏,李佳,杨艳昭,等. 基于SWAT模型的长江源区径流模拟[J].西北林学院学报,2012,27(05):38.
 ZHANG Xiao-yong,LI Jia,YANG Yan-zhao,et al. Runoff Simulation of the Catchment of the Headwaters of the Yangtze River Based on SWAT Model[J].JOURNAL OF NORTHWEST FORESTRY UNIVERSITY,2012,27(5):38.
[3]杨红旗,陈广辉,王金林. PVAc改性酚醛树脂制备铝木复合材料研究[J].西北林学院学报,2013,28(01):170.[doi:10.3969/j.issn.1001-7461.2013.01.34]
 YANG Hong-qi,CHEN Guang-hui,WANG Jin-lin. PF Adhesive Modified by PVAc for Manufacturing Technology of Decorative Fiberboard with Aluminum Foils[J].JOURNAL OF NORTHWEST FORESTRY UNIVERSITY,2013,28(5):170.[doi:10.3969/j.issn.1001-7461.2013.01.34]
[4]江涛,李秀荣,谢延军,等. 微波预处理对巨尾桉木材渗透性的影响[J].西北林学院学报,2013,28(01):174.[doi:10.3969/j.issn.1001-7461.2013.01.35]
 JIANG Tao,LI Xiu-rong,XIE Yan-jun,et al. Effects of Microwave Pretreatment on the Permeability of Eucalyptus grandis×Eucalyptus urophylla[J].JOURNAL OF NORTHWEST FORESTRY UNIVERSITY,2013,28(5):174.[doi:10.3969/j.issn.1001-7461.2013.01.35]
[5]牛晓霆,王逢瑚,曹新民. 明清家具匠师原木下料的工艺原则及措施[J].西北林学院学报,2013,28(01):178.[doi:10.3969/j.issn.1001-7461.2013.01.36]
 NIU Xiao-ting,WANG Feng-hu,CAO Xin-min. Techniques and Principles of Log Cuttings of Furniture Makers in Ming and Qing Dynasties[J].JOURNAL OF NORTHWEST FORESTRY UNIVERSITY,2013,28(5):178.[doi:10.3969/j.issn.1001-7461.2013.01.36]
[6]吕金阳,覃卓凯,舒辉,等. 银杉木材构造美学价值[J].西北林学院学报,2013,28(01):183.[doi:10.3969/j.issn.1001-7461.2013.01.37]
 LV Jin-yang,QIN Zhuo-kai,SHU Hui,et al. Esthetical Values in Wood Structure of Cathaya argyrophylla[J].JOURNAL OF NORTHWEST FORESTRY UNIVERSITY,2013,28(5):183.[doi:10.3969/j.issn.1001-7461.2013.01.37]
[7]杨艳红,张海敏,孙景荣. 基于传统文化视角的现代家具设计探析[J].西北林学院学报,2013,28(01):188.[doi:10.3969/j.issn.1001-7461.2013.01.38]
 YANG Yan-hong,ZHANG Hai-min,SUN Jing-rong. Study of Modern Furniture Design Based on Traditional Culture[J].JOURNAL OF NORTHWEST FORESTRY UNIVERSITY,2013,28(5):188.[doi:10.3969/j.issn.1001-7461.2013.01.38]
[8]黄圣游,叶喜. 新中式家具“中式”传统的界定[J].西北林学院学报,2013,28(01):192.[doi:10.3969/j.issn.1001-7461.2013.01.39]
 HUANG Sheng-You,YE Xi. Definition of Traditional Element in New Chinese Style Furniture[J].JOURNAL OF NORTHWEST FORESTRY UNIVERSITY,2013,28(5):192.[doi:10.3969/j.issn.1001-7461.2013.01.39]
[9]韩维生,赵明磊,王宏斌. 基于阈限理论的设计尺度体系[J].西北林学院学报,2013,28(01):197.[doi:10.3969/j.issn.1001-7461.2013.01.40]
 HAN Wei-sheng,ZHAO Ming-lei,WANG Hong-bin. Design Scale System Based on Threshold Theory[J].JOURNAL OF NORTHWEST FORESTRY UNIVERSITY,2013,28(5):197.[doi:10.3969/j.issn.1001-7461.2013.01.40]
[10]宋杰,侯建军,申黎明. 按摩椅按摩头包覆层对按摩舒适性的影响[J].西北林学院学报,2013,28(01):202.[doi:10.3969/j.issn.1001-7461.2013.01.41]
 SONG Jie,HOU Jian-jun,SHEN Li-ming. Influence of Massager Coat Layer of Massage Chairs on the Degree of Comfort[J].JOURNAL OF NORTHWEST FORESTRY UNIVERSITY,2013,28(5):202.[doi:10.3969/j.issn.1001-7461.2013.01.41]

备注/Memo

备注/Memo:
 收稿日期:2015-11-20修回日期:2016-02-14
基金项目:林业公益性行业科研专项经费项目(201404409)。
作者简介:蔡三山,男,副研究员,研究方向:森林病虫害防治。E-mail:328217811@q.com
*通信作者:陈京元,男,研究员,博士,研究方向:森林保护。E-mail:jingyuanchen@hotmail.com
更新日期/Last Update: