[1]王毛,吴亚飞,邓拓,等. 苏云金芽孢杆菌Cry2Ab基因克隆与植物表达载体的构建[J].西北林学院学报,2016,31(5):178-181.[doi:10.3969/j.issn.1001-7461.2016.05.29]
 WANG Mao,WU Ya-fei,DENT Tuo,et al. Gene Cloning of Cry2Ab from Bacillus thuringiensis and Construction of Plant Expression Vector[J].JOURNAL OF NORTHWEST FORESTRY UNIVERSITY,2016,31(5):178-181.[doi:10.3969/j.issn.1001-7461.2016.05.29]
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 苏云金芽孢杆菌Cry2Ab基因克隆与植物表达载体的构建()
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《西北林学院学报》[ISSN:1001-7461/CN:61-1202/S]

卷:
第31卷
期数:
2016年第5期
页码:
178-181
栏目:
出版日期:
2016-09-30

文章信息/Info

Title:
 Gene Cloning of Cry2Ab from Bacillus thuringiensis and Construction of Plant Expression Vector
文章编号:
1001-7461(2016)05-0178-04
作者:
 王毛吴亚飞邓拓王敦*
 (西北农林科技大学 旱区作物逆境生物学国家重点实验室,陕西 杨陵 712100)
Author(s):
 WANG MaoWU Ya-feiDENT TuoWANG Dun*
 (State Key Laboratory of Crop Stress Biology for Arid Areas,Northwest A&F University,Yangling,Shaanxi 712100,China)
关键词:
 基因克隆苏云金芽胞杆菌Cry2Ab植物表达载体载体构建
Keywords:
 gene clone Bacillus thuringiensis Cry2Ab plant expression vector vector construction
分类号:
S763.13
DOI:
10.3969/j.issn.1001-7461.2016.05.29
文献标志码:
A
摘要:
 以苏云金芽孢杆菌Bt菌液为模版,用PCR扩增的方法克隆出两端带酶切位点XhoⅠ的Cry2Ab片段,与高效植物表达栽体pSR784d连接,并进行PCR和酶切鉴定。结果表明,克隆片段含有Cry2Ab基因、且连接方向正确,所构建植物表达载体Cry2Ab-pSR784d序列与相关元件正确。研究构建单独Cry2Ab植物表达载体,为转基因植物表达和通过转基因植物验证其功能奠定了基础。
Abstract:
 PCR was performed to amplify Cry2Ab gene from Bacillus thuringiensis.Then Cry2Ab gene was ligated to plant expression vector pSR784d with double restriction enzyme sites XhoⅠ.The results showed that the Cry2Ab gene was cloned properly and the expression vector Cry2Ab-pSR784d was constructed successfully.This study constructed a plant expression vector for Cry2Ab transgenic plant and laid foundation for further related studies.

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备注/Memo

备注/Memo:
 收稿日期:2015-11-20修回日期:2016-03-20
基金项目:国家自然科学基金(31270691,31170609);陕西省科技统筹创新工程计划项目(2014KTCL02-14)。
作者简介:王毛,女,在读硕士,研究方向:植物转基因。E-mail:maode2046@163.com
*通信作者:王敦,男,博士,教授,研究方向:昆虫生化与分子生物学、昆虫病毒分子生物学与生物防治。E-mail:wanghande@nwsuaf.edu.cn; dunwang@foxmail.com
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